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[193478_s_at] myosin regulatory light chain 2

Description C56G7.1 /REP_DB=WormBase Gene ID /WP=CE01531 /GEN=mlc-4 /TR=SW:Q09510 /GB=CAA84331.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=myosin regulatory light chain 2
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 99      4grp_highest_8_8 > 06      4grp_high_8_8 > 22      6grp_highest_nonorm > 47      6grp_total > 94      4grp_total > 16      6grp_highest_sub > In      6grp_cgc > 89      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3672
1 2 3 4 range
A 2006.1
(-3)
P 		3155
(-0.4)
P 		2945.1
(-0.9)
P 		4700.6
(3)
P 		2695
B 1904.2
(-3)
P 		2980.4
(-0.8)
P 		3159.9
(-0.5)
P 		4885.4
(3)
P 		2981
C 2035.8
(-3)
P 		2772.7
(-1.8)
P 		2847.4
(-1.6)
P 		5576.3
(3)
P 		3541
avr 1982
(-3) 		2969.4
(-1.1) 		2984.1
(-1) 		5054.1
(3) 		3072

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2063
1 2 3 4 5 6 range
A 2730.5
(-1)
P 		2368.4
(-3)
P 		3378.8
(2.4)
P 		3025.4
(0.5)
P 		3481.7
(3)
P 		3386.1
(2.5)
P 		1113
B 1970.6
(-3)
P 		2865
(-0.3)
P 		3679.9
(2.1)
P 		3147.6
(0.5)
P 		3990.7
(3)
P 		3781.6
(2.4)
P 		2020
C 2537.7
(-2.1)
P 		3126.4
(-0.1)
P 		2289.5
(-3)
P 		2777.4
(-1.3)
P 		4033.9
(3)
P 		3319.8
(0.5)
P 		1744
avr 2412.9
(-3) 		2786.6
(-1.4) 		3116.1
(-0) 		2983.5
(-0.6) 		3835.4
(3) 		3495.8
(1.6) 		1423
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 434
1 2 3 4 range
A 730.4
(0.2)
P 		684.9
(-3)
P 		769.9
(3)
P 		688.1
(-2.8)
P 		85
B 607.2
(-3)
P 		736.1
(-1.2)
P 		732.2
(-1.3)
P 		1041.1
(3)
P 		434
C 673.9
(-3)
P 		797.2
(-0.7)
P 		992.4
(3)
P 		940.3
(2)
P 		319
avr 670.5
(-3) 		739.4
(-1.1) 		831.5
(1.4) 		889.8
(3) 		219

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1118
1 2 3 4 5 6 range
A 503.3
(-3)
P 		842.3
(0.7)
P 		735.8
(-0.5)
P 		767.3
(-0.1)
P 		1050.6
(3)
P 		824.4
(0.5)
P 		547
B 685.7
(-3)
P 		953.6
(-1.3)
P 		893.6
(-1.7)
P 		833.7
(-2.1)
P 		1621.4
(3)
P 		1410.2
(1.6)
P 		936
C 940.6
(0.1)
P 		954
(0.2)
P 		582.7
(-2.8)
P 		558.5
(-3)
P 		1305.3
(3)
P 		1111.3
(1.4)
P 		747
avr 709.9
(-3) 		916.6
(-1) 		737.4
(-2.7) 		719.8
(-2.9) 		1325.8
(3) 		1115.3
(0.9) 		616
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193478_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6830
Sequence Type Exemplar sequence
Representative Public ID C56G7.1 NCBI
Target Description C56G7.1 /REP_DB=WormBase Gene ID /WP=CE01531 /GEN=mlc-4 /TR=SW:Q09510 /GB=CAA84331.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=myosin regulatory light chain 2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065299(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065299 NCBI Caenorhabditis elegans Myosin Light Chain family member (mlc-4) (mlc-4) mRNA, complete cds. 11/11 None
C56G7.1.1 ENSEMBL cdna:known chromosome:CEL140:III:3392501:3393773:-1 gene:C56G7.1 11/11 None
C56G7.1.2 ENSEMBL cdna:known chromosome:CEL140:III:3392522:3393780:-1 gene:C56G7.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026711 2/11 Cross Hyb Matching Probes None
GENEFINDER00000026720 3/11 Cross Hyb Matching Probes None
SNAP00000026712 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3392676-3393767(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin regulatory light chain 2
Gene Symbol mlc-4
Ensembl C56G7.1
Entrez Gene 175440 Entrez gene
SwissProt Q09510 EMBL-EBI
WormBase CE01531 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1583385_X_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
CANINE:1583384_S_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
CANINE:1591821_X_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
CANINE:1591820_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
CANINE:1585604_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
CANINE_2:CFA.12198.1.A1_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
CANINE_2:CFAAFFX.835.1.S1_S_AT similar to Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC) (LC20) cfa
DROSOPHILA_2:1624065_AT spaghetti squash dm
DROSGENOME1:153817_AT spaghetti squash dm
CHICKEN:GGA.4052.1.S1_AT myosin, light polypeptide 9, regulatory gga
HG-U133_PLUS_2:201058_S_AT myosin, light polypeptide 9, regulatory hs
HG-U133A:201058_S_AT myosin, light polypeptide 9, regulatory hs
HG-FOCUS:201058_S_AT myosin, light polypeptide 9, regulatory hs
HG-U133A_2:201058_S_AT myosin, light polypeptide 9, regulatory hs
HUGENEFL:J02854_AT myosin, light polypeptide 9, regulatory hs
U133_X3P:G5174602_3P_X_AT myosin, light polypeptide 9, regulatory hs
U133_X3P:G5174602_3P_AT myosin, light polypeptide 9, regulatory hs
U133_X3P:201058_3P_S_AT myosin, light polypeptide 9, regulatory hs
HG-U95AV2:39145_AT myosin, light polypeptide 9, regulatory hs
MU11KSUBB:MSA.15674.0_F_AT myosin, light polypeptide 9, regulatory mm
MU11KSUBB:MSA.4408.0_F_AT myosin, light polypeptide 9, regulatory mm
MU11KSUBB:MSA.24189.0_F_AT myosin, light polypeptide 9, regulatory mm
MG-U74AV2:96939_AT myosin, light polypeptide 9, regulatory mm
MOE430A:1452670_AT myosin, light polypeptide 9, regulatory mm
MOUSE430A_2:1452670_AT myosin, light polypeptide 9, regulatory mm
MOUSE430_2:1452670_AT myosin, light polypeptide 9, regulatory mm
MU19KSUBC:TC40775_AT myosin, light polypeptide 9, regulatory mm
MU19KSUBC:TC40775_G_AT myosin, light polypeptide 9, regulatory mm
MU11KSUBB:MSA.18350.0_S_AT PREDICTED: myosin regulatory light polypeptide 9 [Mus musculus], mRNA sequence mm
RG-U34A:S77900_G_AT myosin, light polypeptide 9, regulatory (predicted) rn
RG-U34A:S77900_AT myosin, light polypeptide 9, regulatory (predicted) rn
RAE230A:1388298_AT myosin, light polypeptide 9, regulatory (predicted) rn
RAT230_2:1388298_AT myosin, light polypeptide 9, regulatory (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86772 Hypothetical protein C56G7.1 [Caenorhabditis elegans] emb|CAA84331.1| Hypothetical protein C56G7.1 [Caenorhabditis elegans] ref|NP_497700.1| Myosin Light Chain family member (mlc-4) [Caenorhabditis elegans] sp|Q09510|MLRH_CAEEL Probable myosin regulatory light chain 7.0E-95
blast CAE65022 Hypothetical protein CBG09860 [Caenorhabditis briggsae] 7.0E-94
blast XP_623417 PREDICTED: similar to ENSANGP00000021084 [Apis mellifera] ref|XP_623372.1| PREDICTED: similar to ENSANGP00000021084 [Apis mellifera] 3.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.3E-8
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.3E-5
Sequence
Target Sequence 
>C. ELEGANS:193478_S_AT
aacggccacaaagagccacttccaatgtgttcgccatgttcgatcaggctcaaattcaag
agttcaaagaggcattcaacatgatcgaccaaaaccgcgatggattcatcgatcaagagg
atctgaaggatatgttcgcttcccttggaaaagaagtcaccgaacaattcatcgattcga
tgattaacgaggctcccggagcacagccgatcaatttcacaatgttcctgacacttttcg
gtgaaaaattgactggaacggatccggaggaagtcatacggaatgcgttccaatgcttcg
atgaggataattcgggaaaactcaatgaggagcatcttcgcgagttgctcaccacgatgg
gcgagagatattccgaggaacaggttgatgagctctttagagatgctccgattaagggcg
gacaattcgactacgtggagttcactcgtatgctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGGCCACAAAGAGCCACTTCCAA 586 143 44 Antisense
CCACTTCCAATGTGTTCGCCATGTT 112 275 59 Antisense
GTTCGCCATGTTCGATCAGGCTCAA 131 443 72 Antisense
GATATGTTCGCTTCCCTTGGAAAAG 74 425 172 Antisense
GATGATTAACGAGGCTCCCGGAGCA 169 409 222 Antisense
ACAATGTTCCTGACACTTTTCGGTG 39 115 262 Antisense
GGAATGCGTTCCAATGCTTCGATGA 342 529 323 Antisense
GGAGCATCTTCGCGAGTTGCTCACC 233 521 372 Antisense
GTTGCTCACCACGATGGGCGAGAGA 597 439 387 Antisense
AGGGCGGACAATTCGACTACGTGGA 177 59 458 Antisense
TACGTGGAGTTCACTCGTATGCTCA 321 647 475 Antisense
Sequence Source Affymetrix Proprietary Database