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[193468_at] guanine nucleotide-binding protein

Description F30F8.8 /REP_DB=WormBase Gene ID /WP=CE09819 /GEN=taf-4 /TR=O01985 /GB=CAB05603.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_highest_10_10 > 49      6grp_high1_10_10 > 93      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_highest_nonorm > 64      6grp_total > 85      4grp_total > 15      6grp_highest_sub > 04_05_06      6grp_cgc > 77      6grp_high1_sub > 04      6grp_cgc_sub > 04_05_06      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 392
1 2 3 4 range
A 847
(3)
P 		485
(-3)
P 		528.1
(-2.3)
P 		735.9
(1.2)
P 		362
B 793
(1.6)
P 		563.1
(-2.2)
P 		516.5
(-3)
P 		876.7
(3)
P 		360
C 816.4
(3)
P 		602.6
(-3)
P 		696.9
(-0.4)
P 		726.3
(0.5)
P 		214
avr 818.8
(3) 		550.2
(-3) 		580.5
(-2.3) 		779.6
(2.1) 		269

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 330
1 2 3 4 5 6 range
A 729.7
(-2.3)
P 		699.7
(-3)
P 		837.8
(0.4)
P 		943.1
(3)
P 		915.2
(2.3)
P 		813.2
(-0.2)
P 		243
B 734.4
(-2.2)
P 		722.2
(-2.4)
P 		687.7
(-3)
P 		860.7
(0.1)
P 		877
(0.4)
P 		1017.6
(3)
P 		330
C 727.8
(-3)
P 		726.8
(-3)
P 		834.3
(0.8)
P 		897.8
(3)
P 		828.9
(0.6)
P 		834.5
(0.8)
P 		171
avr 730.6
(-2.5) 		716.2
(-3) 		786.6
(-0.7) 		900.5
(3) 		873.7
(2.1) 		888.4
(2.6) 		184
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 308.4
(3)
P 		105.3
(-3)
P 		138.1
(-2)
P 		107.7
(-2.9)
P 		203
B 252.9
(3)
P 		139.1
(-2.1)
P 		119.7
(-3)
P 		186.8
(0)
P 		133
C 270.3
(3)
P 		173.3
(-0.9)
P 		242.9
(1.9)
P 		122.5
(-3)
P 		148
avr 277.2
(3) 		139.2
(-3) 		166.9
(-1.8) 		139
(-3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 134.5
(-3)
P 		248.9
(1.8)
P 		182.5
(-1)
P 		239.2
(1.4)
P 		276.2
(3)
P 		198
(-0.3)
P 		142
B 255.5
(-0.5)
P 		240.4
(-0.9)
P 		167
(-3)
P 		228
(-1.3)
P 		356.3
(2.3)
P 		379.5
(3)
P 		213
C 269.8
(2.4)
P 		221.8
(-0.5)
P 		212.3
(-1.1)
P 		180.6
(-3)
P 		268.2
(2.3)
P 		279.4
(3)
P 		99
avr 219.9
(-1.3) 		237
(-0.4) 		187.3
(-3) 		215.9
(-1.5) 		300.2
(3) 		285.6
(2.2) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193468_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1654
Sequence Type Exemplar sequence
Representative Public ID F30F8.8 NCBI
Target Description F30F8.8 /REP_DB=WormBase Gene ID /WP=CE09819 /GEN=taf-4 /TR=O01985 /GB=CAB05603.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059768(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059768 NCBI Caenorhabditis elegans TAF (TBP-associated transcription factor) family member (taf-5) (taf-5) mRNA, complete cds. 11/11 None
SNAP00000037580 ENSEMBL cdna:SNAP chromosome:CEL140:I:7859932:7861624:1 10/11 None
GENEFINDER00000037590 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7859981:7861624:1 10/11 None
F30F8.8.1 ENSEMBL cdna:known chromosome:CEL140:I:7857101:7861749:1 gene:F30F8.8 11/11 None
F30F8.8.3 ENSEMBL cdna:known chromosome:CEL140:I:7857742:7861624:1 gene:F30F8.8 11/11 None
F30F8.8.2 ENSEMBL cdna:known chromosome:CEL140:I:7857939:7861749:1 gene:F30F8.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7857881-7861555(+) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol taf-5
Ensembl F30F8.8
Entrez Gene 172552 Entrez gene
SwissProt O01985 EMBL-EBI
WormBase CE09819 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
45449 regulation of transcription inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
30528 transcription regulator activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03035 Hypothetical protein F30F8.8 [Caenorhabditis elegans] emb|CAB05603.1| Hypothetical protein F30F8.8 [Caenorhabditis elegans] ref|NP_492169.1| TAF (TBP-associated transcription factor) family member (taf-5) [Caenorhabditis elegans] 0.0
blast CAE56124 Hypothetical protein CBG23733 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007582 EMBL-EBI WD40 associated region in TFIID subunit 5.7E-66
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.2E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.021
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.4E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.4E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.2E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0035
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.4E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.4E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.2E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0035
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.4E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.4E-7
Sequence
Target Sequence 
>C. ELEGANS:193468_AT
gtagctcaattctgtagtcgtggatactatttcgctacagcaagtgccgataaaacagct
gcaatgtggtcgacagatcgaatgcatccacttagaatattcgctgatccatatggagac
gttggatgtattgattatcatccaaattgtaattatattgctggaggatcagatgataga
tatgttcgagtatgggatgtttgttctggtactcgagttcgcatttttagtggtcataaa
gcttcgattatcgctgtcaagttctccccatgtggtcggtatatcgtctcgttagatgct
attggaaatctgatgatttgggatttggcataccagagattagttgcagctgaaattacg
gaacaagctggaacgaagggttcaattacattctctcgtgatggtggtgtatttgcggta
tcccatggaaattcaagtattcaattatattcacttgatactcttattggaacagttctc
gccgctggacaaaatgattcctacattgagccaaaagtgaatctggacggtttcaatata
ggatcatatgcaacaaaagaaactgcagtaatcggactccatttcactcgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGCTCAATTCTGTAGTCGTGGAT 54 457 1321 Antisense
GTCGTGGATACTATTTCGCTACAGC 661 471 1337 Antisense
GACAGATCGAATGCATCCACTTAGA 241 369 1392 Antisense
TGTTTGTTCTGGTACTCGAGTTCGC 349 561 1518 Antisense
TCGAGTTCGCATTTTTAGTGGTCAT 23 603 1533 Antisense
TTCGATTATCGCTGTCAAGTTCTCC 469 695 1563 Antisense
GTGGTCGGTATATCGTCTCGTTAGA 269 489 1592 Antisense
AAGGGTTCAATTACATTCTCTCGTG 634 159 1696 Antisense
GGTGTATTTGCGGTATCCCATGGAA 361 499 1726 Antisense
ACAGTTCTCGCCGCTGGACAAAATG 452 107 1792 Antisense
AGTAATCGGACTCCATTTCACTCGT 471 61 1887 Antisense
Sequence Source Affymetrix Proprietary Database