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[193467_s_at]

Description C16C2.2 /REP_DB=WormBase Gene ID /WP=CE17404 /GEN=eat-16 /TR=O17589 /GB=CAB02742.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=regulator of G-protein signalling
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 02      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 24      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 688
1 2 3 4 range
A 943.1
(3)
P 		423.9
(-1.8)
P 		378.1
(-2.2)
P 		291.9
(-3)
P 		651
B 757.7
(3)
P 		424.8
(-1.4)
P 		303.1
(-3)
P 		399.4
(-1.7)
P 		455
C 847.3
(3)
P 		537.7
(-0.1)
P 		382.7
(-1.7)
P 		255
(-3)
P 		592
avr 849.4
(3) 		462.1
(-1.4) 		354.6
(-2.6) 		315.4
(-3) 		534

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 564
1 2 3 4 5 6 range
A 484.9
(2)
P 		541.7
(3)
P 		393.3
(0.4)
P 		369.4
(-0.1)
P 		217.7
(-2.8)
P 		204
(-3)
P 		338
B 529.7
(3)
P 		417
(1)
P 		385.8
(0.5)
P 		294.4
(-1.1)
P 		185.9
(-3)
P 		228.6
(-2.3)
P 		344
C 750.1
(3)
P 		392.6
(-1)
P 		422.4
(-0.6)
P 		329.7
(-1.7)
P 		258.6
(-2.5)
P 		209.4
(-3)
P 		541
avr 588.2
(3) 		450.4
(0.8) 		400.5
(-0) 		331.2
(-1.1) 		220.7
(-2.9) 		214
(-3) 		374
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 343.4
(3)
P 		92
(-2)
P 		98.8
(-1.9)
P 		42.7
(-3)
P 		301
B 241.6
(3)
P 		104.9
(-1.8)
P 		70.2
(-3)
P 		85.1
(-2.5)
P 		171
C 280.5
(3)
P 		154.6
(-0.2)
P 		133.4
(-0.7)
P 		43
(-3)
P 		238
avr 288.5
(3) 		117.2
(-1.4) 		100.8
(-1.9) 		56.9
(-3) 		232

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 89.4
(-1.3)
P 		192.7
(3)
P 		85.6
(-1.5)
P 		93.7
(-1.2)
P 		65.7
(-2.3)
P 		49.7
(-3)
P 		143
B 184.3
(3)
P 		138.8
(0.5)
P 		93.7
(-2)
P 		78
(-2.9)
P 		75.5
(-3)
P 		85.3
(-2.5)
P 		109
C 278
(3)
P 		119.8
(-1.5)
P 		107.5
(-1.8)
P 		66.3
(-3)
P 		83.7
(-2.5)
P 		70.1
(-2.9)
P 		212
avr 183.9
(3) 		150.4
(1.3) 		95.6
(-1.6) 		79.3
(-2.4) 		75
(-2.7) 		68.4
(-3) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193467_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.895
Sequence Type Exemplar sequence
Representative Public ID C16C2.2 NCBI
Target Description C16C2.2 /REP_DB=WormBase Gene ID /WP=CE17404 /GEN=eat-16 /TR=O17589 /GB=CAB02742.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=regulator of G-protein signalling
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170911(11), NM_170912(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170911 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-16) (eat-16) mRNA, complete cds. 11/11 None
NM_170912 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-16) (eat-16) mRNA, complete cds. 11/11 None
GENEFINDER00000032655 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9730925:9733737:1 11/11 None
SNAP00000032651 ENSEMBL cdna:SNAP chromosome:CEL140:I:9732403:9733271:1 11/11 None
C16C2.2a.2 ENSEMBL cdna:known chromosome:CEL140:I:9730676:9734411:1 gene:C16C2.2 11/11 None
C16C2.2a.1 ENSEMBL cdna:known chromosome:CEL140:I:9730842:9734411:1 gene:C16C2.2 11/11 None
C16C2.2b ENSEMBL cdna:known chromosome:CEL140:I:9730925:9733613:1 gene:C16C2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9730855-9733469(+) 100.0 100.0
Public Domain and Genome References
Ensembl C16C2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02742 Hypothetical protein C16C2.2a [Caenorhabditis elegans] ref|NP_740904.1| EATing: abnormal pharyngeal pumping family member (eat-16) [Caenorhabditis elegans] 0.0
blast CAE60241 Hypothetical protein CBG03814 [Caenorhabditis briggsae] 0.0
blast CAD30433 Hypothetical protein C16C2.2b [Caenorhabditis elegans] ref|NP_740905.1| EATing: abnormal pharyngeal pumping family member (eat-16) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001770 EMBL-EBI G-protein, gamma subunit 2.1E-26
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 3.7E-23
Pfam IPR000342 EMBL-EBI Regulator of G protein 8.1E-29
Pfam IPR001770 EMBL-EBI G-protein, gamma subunit 2.0E-13
Pfam IPR000342 EMBL-EBI Regulator of G protein 8.1E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000032655 1 494-513
Sequence
Target Sequence 
>C. ELEGANS:193467_S_AT
tgacacgtcaagtgccgagatccctaccgagaaacgggtaaaacgatggggactttctgt
tcaagagcttgtgaaagatcctataggacggcaagtgctcgaaacatttttagaatccga
attttcgtcggaaaatatacggttctggatagcgatacaagatttgaaatatgcaccgaa
tgagcagatataccagaaagctgaaagaatacgagaagaatttttggctcaaggagcacc
tgcacaagtaaatgtagacaatagaaccctcgatcagacattggagtgtatttcgaaagc
gaaagatgcttcacaaatgagattcgcattctatcattctgaagagcacgtgttcacatt
gatggcaaaggattcatatccacgtttcgtccgatcccaaatctacaaagcagtattgac
agcagcgcaacagcacggaacaaagcgactcgggtggcgcaacttcgtattcaacatggg
tacaactaaaaaaccagcaacgagtaaaccagcaaagccgcaagattccatcgggactgg
tcaagttctcccaaaacagctatcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACACGTCAAGTGCCGAGATCCCT 572 571 816 Antisense
GGGGACTTTCTGTTCAAGAGCTTGT 556 497 863 Antisense
AATAGAACCCTCGATCAGACATTGG 265 165 1075 Antisense
GAGATTCGCATTCTATCATTCTGAA 167 397 1134 Antisense
AAGGATTCATATCCACGTTTCGTCC 376 159 1183 Antisense
TTTCGTCCGATCCCAAATCTACAAA 150 671 1200 Antisense
GTATTGACAGCAGCGCAACAGCACG 629 451 1228 Antisense
CAAAGCGACTCGGGTGGCGCAACTT 625 189 1256 Antisense
GGCGCAACTTCGTATTCAACATGGG 276 541 1271 Antisense
GATTCCATCGGGACTGGTCAAGTTC 425 433 1339 Antisense
AAGTTCTCCCAAAACAGCTATCGTC 283 157 1358 Antisense
Sequence Source Affymetrix Proprietary Database