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[193462_at] guanine nucleotide-binding protein

Description T19C4.6 /REP_DB=WormBase Gene ID /WP=CE06470 /GEN=gpa-1 /TR=Q22567 /GB=CAA99914.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_high_8_8 > 25      6grp_highest_nonorm > 90      6grp_total > 30      4grp_total > 39      6grp_highest_sub > 02      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 261
1 2 3 4 range
A 278
(3)
P 		107.4
(-2.8)
P 		101.9
(-3)
A 		130.6
(-2)
P 		176
B 329.7
(3)
P 		126.1
(-1.7)
P 		68.6
(-3)
A 		153.5
(-1)
P 		261
C 314.2
(3)
P 		90.7
(-2.8)
A 		83.7
(-3)
P 		228.3
(0.8)
P 		231
avr 307.3
(3) 		108.1
(-2.4) 		84.7
(-3) 		170.8
(-0.7) 		223

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 167.1
(1.5)
P 		195
(3)
P 		186.3
(2.5)
P 		174.9
(1.9)
P 		84.2
(-3)
P 		109.5
(-1.6)
P 		111
B 158.7
(-3)
P 		186.5
(2)
P 		192.3
(3)
P 		191
(2.8)
P 		162.4
(-2.3)
P 		165.1
(-1.9)
P 		34
C 162.1
(-0)
P 		211
(3)
P 		182
(1.2)
P 		138.4
(-1.5)
P 		113.7
(-3)
P 		195.9
(2.1)
P 		97
avr 162.6
(0.3) 		197.5
(3) 		186.9
(2.2) 		168.1
(0.7) 		120.1
(-3) 		156.8
(-0.2) 		77
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 101.2
(3)
P 		23.3
(-2.7)
P 		26.6
(-2.5)
A 		19.1
(-3)
P 		82
B 105.1
(3)
P 		31.1
(-2)
P 		15.9
(-3)
A 		32.7
(-1.9)
P 		89
C 104
(3)
P 		26.1
(-3)
A 		29.2
(-2.8)
P 		38.5
(-2)
P 		78
avr 103.4
(3) 		26.8
(-2.8) 		23.9
(-3) 		30.1
(-2.5) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 30.8
(-2.3)
P 		69.3
(3)
P 		40.6
(-0.9)
P 		44.4
(-0.4)
P 		25.4
(-3)
P 		26.7
(-2.8)
P 		44
B 55.2
(-0.4)
P 		62.1
(1.8)
P 		46.7
(-3)
P 		50.6
(-1.8)
P 		66
(3)
P 		61.6
(1.6)
P 		19
C 60.1
(2.1)
P 		64.4
(2.8)
P 		46.3
(-0.1)
P 		27.8
(-3)
P 		36.8
(-1.6)
P 		65.6
(3)
P 		38
avr 48.7
(-1.1) 		65.3
(3) 		44.5
(-2.1) 		40.9
(-3) 		42.7
(-2.6) 		51.3
(-0.4) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193462_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13979
Sequence Type Exemplar sequence
Representative Public ID T19C4.6 NCBI
Target Description T19C4.6 /REP_DB=WormBase Gene ID /WP=CE06470 /GEN=gpa-1 /TR=Q22567 /GB=CAA99914.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanine nucleotide-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073439(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073439 NCBI Caenorhabditis elegans G Protein, Alpha subunit family member (gpa-1) (gpa-1) mRNA, complete cds. 11/11 None
SNAP00000015940 ENSEMBL cdna:SNAP chromosome:CEL140:V:11177155:11180147:-1 11/11 None
GENEFINDER00000015942 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11177155:11180147:-1 11/11 None
T19C4.6 ENSEMBL cdna:known chromosome:CEL140:V:11177155:11180147:-1 gene:T19C4.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11177154-11180147(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine nucleotide-binding protein
Gene Symbol gpa-1
Ensembl T19C4.6
Entrez Gene 179546 Entrez gene
SwissProt P28051 EMBL-EBI
WormBase CE06470 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA99914 Hypothetical protein T19C4.6 [Caenorhabditis elegans] ref|NP_505840.1| G Protein, Alpha subunit family member (gpa-1) [Caenorhabditis elegans] gb|AAG32077.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|P28051|GPA1_CAEEL Guanine nucleotide-binding protein alpha-1 subunit 0.0
blast AAA28060 G protein alpha subunit 0.0
blast CAA99914 Hypothetical protein T19C4.6 [Caenorhabditis elegans] ref|NP_505840.1| G Protein, Alpha subunit family member (gpa-1) [Caenorhabditis elegans] gb|AAG32077.1| heterotrimeric G protein alpha subunit [Caenorhabditis elegans] sp|P28051|GPA1_CAEEL Guanine nucleotide-binding protein alpha-1 subunit 1.0E-171
blast AAA28060 G protein alpha subunit 1.0E-169
blast AAW02893 gpa-1 [Caenorhabditis briggsae] sp|Q60MJ0|GPA1_CAEBR Guanine nucleotide-binding protein alpha-1 subunit emb|CAE75186.1| Hypothetical protein CBG23127 [Caenorhabditis briggsae] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 8.6E-9
Pfam IPR001019 EMBL-EBI Guanine nucleotide binding protein (G-protein), alpha subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193462_AT
aattgcaagggttcacactcccaattacgtaccaactgaaaatgatattctgcatacacg
ggttccaacaatgggtgttatagaagttaatttcacaataaagggcaaattttttcgagt
atttgatgtgggcggacagcgatcgcaaaggaagaaatggattcactgttttgatgatgc
aaaagctatgatatatgttgcttcgttgtcagagtatgatcaagttttattagaagacaa
tactactaatcgaatgcacgaatcaatacagttatttaagcaagtaatcaacaataaata
ttttgtaaacacttcagttatattattcctgaacaaaattgatttattcgaggaaaaaat
tgttaccaaaaaacgaagtcttggtattgcatttgagtcatttagtggaccgagccaaga
tctcaatgcagctgtagcgttcgttgaaaaaaagtatagaagtatggcagagaacaaaga
gaagaacatttattgtcatcacacttgtgctacagacacacaacaggtgcagtacgtact
cgatgcggttctagat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTGCAAGGGTTCACACTCCCAAT 460 177 489 Antisense
CACTCCCAATTACGTACCAACTGAA 18 199 504 Antisense
TATTCTGCATACACGGGTTCCAACA 194 665 534 Antisense
GATGTGGGCGGACAGCGATCGCAAA 328 407 613 Antisense
GATATATGTTGCTTCGTTGTCAGAG 576 425 678 Antisense
GAAGTCTTGGTATTGCATTTGAGTC 689 335 863 Antisense
GTGGACCGAGCCAAGATCTCAATGC 333 491 893 Antisense
ATCTCAATGCAGCTGTAGCGTTCGT 156 31 908 Antisense
GTCATCACACTTGTGCTACAGACAC 656 467 983 Antisense
ACACAACAGGTGCAGTACGTACTCG 315 111 1006 Antisense
GTACGTACTCGATGCGGTTCTAGAT 455 457 1020 Antisense
Sequence Source Affymetrix Proprietary Database