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[193456_at] sex-determining protein (FEM-3)

Description C01F6.4 /REP_DB=WormBase Gene ID /WP=CE02953 /GEN=fem-3 /TR=SW:P34691 /GB=CAA92437.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=sex-determining protein (FEM-3)
Cluster 6grp_total > 96      4grp_total > 16      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 49.5
(-1.9)
A
23.3
(-3)
A
70
(-1)
A
161.8
(3)
A
139
B 20.3
(-3)
A
92.7
(3)
A
46.9
(-0.8)
A
62.7
(0.5)
A
72
C 77.1
(-3)
A
83.8
(-2.1)
A
97.8
(-0.1)
A
120.5
(3)
A
43
avr 49
(-3)
66.6
(-1.4)
71.6
(-0.9)
115
(3)
66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 356
1 2 3 4 5 6 range
A 105.4
(-2.3)
A
62.4
(-3)
A
95.5
(-2.4)
A
154.7
(-1.4)
A
330.8
(1.5)
P
418.1
(3)
P
356
B 71.2
(-3)
A
72.5
(-3)
A
72
(-3)
A
125.9
(-2)
A
315.7
(1.5)
P
394.3
(3)
P
323
C 81.5
(-2.7)
A
65.4
(-3)
A
94.2
(-2.5)
A
221.8
(-0.1)
A
316.2
(1.7)
P
385.7
(3)
P
320
avr 86
(-2.7)
66.8
(-3)
87.2
(-2.6)
167.5
(-1.2)
320.9
(1.6)
399.4
(3)
333
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 18
(1.2)
A
5.1
(-3)
A
18.3
(1.3)
A
23.7
(3)
A
19
B 6.5
(-3)
A
22.9
(3)
A
10.9
(-1.4)
A
13.4
(-0.5)
A
16
C 25.5
(-0.7)
A
24.1
(-1.3)
A
34.1
(3)
A
20.3
(-3)
A
14
avr 16.7
(-3)
17.4
(-2)
21.1
(3)
19.1
(0.3)
4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 19.4
(-3)
A
22.2
(-2.8)
A
20.8
(-2.9)
A
39.2
(-1.6)
A
99.8
(2.9)
P
101.8
(3)
P
82
B 24.8
(-2.7)
A
24.1
(-2.7)
A
17.5
(-3)
A
33.3
(-2.3)
A
128.3
(2.1)
P
147.1
(3)
P
130
C 30.2
(-2.4)
A
20
(-3)
A
24
(-2.8)
A
44.6
(-1.6)
A
102.3
(1.5)
P
129.1
(3)
P
109
avr 24.8
(-2.8)
22.1
(-2.9)
20.8
(-3)
39
(-2)
110.1
(2.1)
126
(3)
105
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193456_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10157
Sequence Type Exemplar sequence
Representative Public ID C01F6.4 NCBI
Target Description C01F6.4 /REP_DB=WormBase Gene ID /WP=CE02953 /GEN=fem-3 /TR=SW:P34691 /GB=CAA92437.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=sex-determining protein (FEM-3)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069186(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_069186 NCBI Caenorhabditis elegans FEMinization of XX and XO animals family member (fem-3) (fem-3) mRNA, complete cds. 11/11 None
SNAP00000031440 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9105182:9107324:1 11/11 None
GENEFINDER00000031446 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9105182:9107324:1 11/11 None
C01F6.4 ENSEMBL cdna:known chromosome:CEL140:IV:9104946:9107589:1 gene:C01F6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9105187-9107330(+) 100.0 100.0
Public Domain and Genome References
Gene Title sex-determining protein (FEM-3)
Gene Symbol fem-3
Ensembl C01F6.4
Entrez Gene 177734 Entrez gene
SwissProt P34691 EMBL-EBI
WormBase CE02953 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92437 Hypothetical protein C01F6.4 [Caenorhabditis elegans] ref|NP_501587.1| FEMinization of XX and XO animals family member (fem-3) [Caenorhabditis elegans] emb|CAA46125.1| sex determination protein [Caenorhabditis elegans] emb|CAA46126.1| unnamed protein product [Caenorhabditis elegans] sp|P34691|FEM3_CAEEL Sex-determination protein fem-3 (Feminization of XX and XO animals protein 3) (Ce-FEM-3) 0.0
blast AAN37405 cytoplasmic sex determination protein [Caenorhabditis briggsae] 7.0E-65
blast Q8I8U6 Sex-determination protein fem-3 (Cb-FEM-3) emb|CAE74113.1| Hypothetical protein CBG21774 [Caenorhabditis briggsae] 7.0E-65
Sequence
Target Sequence
>C. ELEGANS:193456_AT
ctcttcttcatacactctatttcaatattccgggaatgccacagttttggaacaattccc
aaatgtataattacgctgctacccgtggtcaacgccttgttcaaaatatcgccgcttttt
atccacccgaatatttttggaacgaagatgaatccaagtatcatactacgtttgtggttc
cacgtggcacagagttctcaaagttttatgctcgaagatttcatgaagcacttggaatgc
caccactggaaaatgagattattacggttttggattggcttgcgaagctctgcattttgg
aaattgtatatcacacaacgatttggtgcgacataactggatttggagggcttccacgta
ttgagcactatcgtttagccatggagaatgttgaagacatcatattcgacttggcaattg
atgatttctcaatttcccgtctacagttacaaatttcgccgtttgaaatctctcgcta


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
CTCTTCTTCATACACTCTATTTCAA 686 229 554 Antisense
ATATTCCGGGAATGCCACAGTTTTG 263 17 578 Antisense
CAAAATATCGCCGCTTTTTATCCAC 181 191 655 Antisense
TATCATACTACGTTTGTGGTTCCAC 658 659 712 Antisense
TGGTTCCACGTGGCACAGAGTTCTC 458 551 728 Antisense
GCACTTGGAATGCCACCACTGGAAA 165 309 781 Antisense
TTACGGTTTTGGATTGGCTTGCGAA 481 681 815 Antisense
GGCTTGCGAAGCTCTGCATTTTGGA 480 537 830 Antisense
GGGCTTCCACGTATTGAGCACTATC 632 493 901 Antisense
CTCAATTTCCCGTCTACAGTTACAA 17 229 981 Antisense
TTTCGCCGTTTGAAATCTCTCGCTA 204 671 1007 Antisense
Sequence Source Affymetrix Proprietary Database