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[193452_at]

Description R53.3A /REP_DB=WormBase Gene ID /WP=CE03572 /GEN=egl-43 /TR=Q22024 /GB=CAA91353.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Zinc finger, C2H2 type (4 domains)
Cluster 6grp_total > 43      4grp_total > 41      6grp_cgc > 91      6grp_cgc_sub > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 4.2
(-3)
A 		4.9
(-1)
A 		6.3
(3)
A 		6
(2.1)
A 		2
B 3.4
(-3)
A 		41
(2.1)
A 		47.7
(3)
A 		5.2
(-2.8)
A 		44
C 9.6
(-3)
A 		13.7
(-2.2)
A 		11.8
(-2.5)
A 		38.9
(3)
A 		29
avr 5.7
(-3) 		19.9
(2.3) 		21.9
(3) 		16.7
(1.1) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 20.9
(2)
A 		10
(-0.9)
A 		24.5
(3)
A 		4.3
(-2.5)
A 		8.6
(-1.3)
A 		2.3
(-3)
A 		22
B 8.9
(3)
A 		6.9
(1.4)
A 		4.1
(-0.9)
A 		8.7
(2.8)
A 		1.6
(-3)
A 		1.7
(-2.9)
A 		7
C 15.1
(-1.4)
A 		2.3
(-3)
A 		50
(3)
A 		6.7
(-2.4)
A 		4.7
(-2.7)
A 		26.8
(0.1)
A 		48
avr 15
(-0.2) 		6.4
(-2.6) 		26.2
(3) 		6.6
(-2.5) 		5
(-3) 		10.3
(-1.5) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 1.5
(1.5)
A 		1.1
(-1.5)
A 		1.7
(3)
A 		0.9
(-3)
A 		1
B 1.1
(-3)
A 		10.1
(2.4)
A 		11.1
(3)
A 		1.1
(-3)
A 		10
C 3.2
(-3)
A 		3.9
(-1.8)
A 		4.1
(-1.4)
A 		6.6
(3)
A 		3
avr 1.9
(-3) 		5
(2) 		5.6
(3) 		2.9
(-1.4) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 3.9
(1.2)
A 		3.6
(0.8)
A 		5.3
(3)
A 		1.1
(-2.4)
A 		2.6
(-0.4)
A 		0.6
(-3)
A 		5
B 3.1
(3)
A 		2.3
(1.1)
A 		1
(-2)
A 		2.3
(1.1)
A 		0.7
(-2.8)
A 		0.6
(-3)
A 		3
C 5.6
(-0.6)
A 		0.7
(-3)
A 		12.7
(3)
A 		1.3
(-2.7)
A 		1.5
(-2.6)
A 		9
(1.2)
A 		12
avr 4.2
(0.3) 		2.2
(-2.2) 		6.3
(3) 		1.6
(-3) 		1.6
(-3) 		3.4
(-0.7) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193452_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2616
Sequence Type Exemplar sequence
Representative Public ID R53.3A NCBI
Target Description R53.3A /REP_DB=WormBase Gene ID /WP=CE03572 /GEN=egl-43 /TR=Q22024 /GB=CAA91353.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Zinc finger, C2H2 type (4 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NR_002363(11), NM_001027117(11), NM_063748(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NR_002363 NCBI Caenorhabditis elegans egl-43, miscRNA. 11/11 None
NM_001027117 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-43) (egl-43) mRNA, complete cds. 11/11 None
NM_063748 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-43) (egl-43) mRNA, complete cds. 11/11 None
GENEFINDER00000040710 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9954946:9962180:1 9/11 None
R53.3a ENSEMBL cdna:known chromosome:CEL140:II:9955096:9962894:1 gene:R53.3 11/11 None
R53.3b ENSEMBL cdna:known chromosome:CEL140:II:9960245:9962065:1 gene:R53.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000040699 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9955093-9962062(+) 100.0 100.0
Public Domain and Genome References
Ensembl R53.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91353 Hypothetical protein R53.3a [Caenorhabditis elegans] ref|NP_001022288.1| EGg Laying defective family member (egl-43) [Caenorhabditis elegans] 0.0
blast AAB28819 Egl-43 [Caenorhabditis elegans] 0.0
blast CAE57950 Hypothetical protein CBG01007 [Caenorhabditis briggsae] 1.0E-174
blast CAE57950 Hypothetical protein CBG01007 [Caenorhabditis briggsae] 1.0E-164
blast CAA91352 Hypothetical protein R53.3b [Caenorhabditis elegans] ref|NP_496149.3| EGg Laying defective family member (egl-43) [Caenorhabditis elegans] 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 6.0E-5
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 6.7E-5
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 2.4E-7
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 3.0E-6
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 2.4E-7
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 3.0E-6
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 6.0E-5
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 6.7E-5
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 2.4E-7
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 3.0E-6
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 3.4E-5
Sequence
Target Sequence 
>C. ELEGANS:193452_AT
ggatcgtgacagcatcggagactctgggaatgatgatgatgatgactcagaagctggagt
tctagatgagtcgtccacaacaacgtcaacgaaaaagcgtccgacatctcacacaatttc
cgacatactcgctgctccacaactcggtgctcaggctttgaattcgacgttccttggcat
gcttcagcgctcgcttaattataatccagcagttccatcgcctcactcatttctaagagc
aatgagcggagcaaaagcatcatcatcaccaagctcgagcagtggaagtggaaaggatag
gtacacgtgcaagttctgtcagaaagtgttcccaagatcagcgaatttgacaagacattt
aaggacacatacaggagagcagccttataagtgtcaatattgcgagcgaagcttctcaat
ctcctccaatttgcaacgccacgttagaaacattcataataagccgaacacctcgctgac
accccacaatcatcatcgtcaacgaagtctgcacaattcaacctcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCGTGACAGCATCGGAGACTCT 79 513 1041 Antisense
TGAGTCGTCCACAACAACGTCAACG 637 565 1107 Antisense
GACATCTCACACAATTTCCGACATA 45 371 1143 Antisense
ACAACTCGGTGCTCAGGCTTTGAAT 624 111 1179 Antisense
TTTGAATTCGACGTTCCTTGGCATG 682 665 1197 Antisense
TTATAATCCAGCAGTTCCATCGCCT 366 679 1239 Antisense
CATCGCCTCACTCATTTCTAAGAGC 352 211 1256 Antisense
TCATCATCACCAAGCTCGAGCAGTG 81 621 1300 Antisense
TTGCGAGCGAAGCTTCTCAATCTCC 337 703 1440 Antisense
TAATAAGCCGAACACCTCGCTGACA 134 635 1497 Antisense
GAAGTCTGCACAATTCAACCTCAAC 220 337 1544 Antisense
Sequence Source Affymetrix Proprietary Database