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[193426_at]

Description C18F10.4 /REP_DB=WormBase Gene ID /WP=CE00781 /GEN=srg-1 /TR=SW:P46570 /GB=AAC47053.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=seven-transmembrane receptor
Cluster 6grp_total > 73      4grp_total > 07      6grp_cgc > 65      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 26.2
(-3)
A 		39.9
(-1.9)
A 		50.3
(-1.1)
A 		100.9
(3)
M 		75
B 53.9
(3)
A 		9.2
(-3)
A 		49.7
(2.4)
A 		15.8
(-2.1)
A 		45
C 61.3
(2.5)
A 		10.5
(-3)
A 		28.1
(-1.1)
A 		65.5
(3)
A 		55
avr 47.1
(1) 		19.9
(-3) 		42.7
(0.4) 		60.7
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 20.5
(-2.9)
A 		19.9
(-3)
A 		48.3
(2.3)
A 		33.8
(-0.4)
A 		51.8
(3)
A 		46.6
(2)
A 		32
B 19.5
(-2.4)
A 		39.7
(1.1)
A 		34.8
(0.2)
A 		50.9
(3)
A 		50.1
(2.9)
A 		16.3
(-3)
A 		35
C 10
(-2.4)
A 		64.9
(3)
A 		33.7
(-0.1)
A 		20.2
(-1.4)
A 		3.9
(-3)
A 		29.5
(-0.5)
A 		61
avr 16.7
(-3) 		41.5
(3) 		38.9
(2.4) 		35
(1.4) 		35.3
(1.5) 		30.8
(0.4) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 9.5
(-2.2)
A 		8.7
(-3)
A 		13.2
(1.4)
A 		14.8
(3)
M 		6
B 17.2
(3)
A 		2.3
(-3)
A 		11.5
(0.7)
A 		3.4
(-2.6)
A 		15
C 20.3
(3)
A 		3
(-3)
A 		9.8
(-0.6)
A 		11
(-0.2)
A 		17
avr 15.7
(3) 		4.7
(-3) 		11.5
(0.7) 		9.7
(-0.3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 3.8
(-3)
A 		7.1
(-1.3)
A 		10.5
(0.4)
A 		8.6
(-0.6)
A 		15.6
(3)
A 		11.3
(0.8)
A 		12
B 6.8
(-2.7)
A 		13.2
(-0)
A 		8.4
(-2)
A 		13.5
(0.1)
A 		20.4
(3)
A 		6.1
(-3)
A 		14
C 3.7
(-2.2)
A 		19.8
(3)
A 		8.6
(-0.6)
A 		4.1
(-2.1)
A 		1.3
(-3)
A 		9.9
(-0.2)
A 		19
avr 4.8
(-3) 		13.4
(3) 		9.2
(0.1) 		8.7
(-0.3) 		12.4
(2.3) 		9.1
(-0) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193426_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6521
Sequence Type Exemplar sequence
Representative Public ID C18F10.4 NCBI
Target Description C18F10.4 /REP_DB=WormBase Gene ID /WP=CE00781 /GEN=srg-1 /TR=SW:P46570 /GB=AAC47053.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=seven-transmembrane receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065965
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000044243 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6257075:6258301:-1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_065965 7/11 Cross Hyb Matching Probes None
SNAP00000044232 4/11 Cross Hyb Matching Probes None
C18F10.4 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6257073-6258300(-) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast 2123261AA chemosensory receptor 1.0E-124
blast AAC47053 Serpentine receptor, class g (gamma) protein 1 [Caenorhabditis elegans] ref|NP_498366.2| Serpentine Receptor, class G (gamma) family member (srg-1) [Caenorhabditis elegans] sp|P46570|SRG1_CAEEL Serpentine receptor class gamma-1 (Protein srg-1) 1.0E-114
blast AAC47052 Serpentine receptor, class g (gamma) protein 2 [Caenorhabditis elegans] ref|NP_498367.2| Serpentine Receptor, class G (gamma) family member (srg-2) [Caenorhabditis elegans] sp|P46571|SRG2_CAEEL Serpentine receptor class gamma-2 (Protein srg-2) 9.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000044243 6 25-47,59-81,118-140,147-169,189-211,232-254
Sequence
Target Sequence 
>C. ELEGANS:193426_AT
tcgagcttcttgtgtgttatggccattaagctattcgctattttggaagaagtggttgaa
gtcgattctaacgacaatggctatttcgccatgcttatggatatggactattgcaatttc
agataaaatgatagttcatggatatggtggtcttgtagtgttatactatagatatgtttc
atgggcacgaagcacattattcttttcaattctccgtctgacatcagtaataacaatagt
agttgcgacaactacaatgttgatcaaaatgtctagaatgaagaagagaattcgggaatc
tgaacggagactttgttgggcatcggtgtatctatcagtttgttatctactccctgcaat
tgcagaggtcatcttcaaacccatcagattagatgttataatcttaatttcagttcgaat
acttcctggtcttaaaagcgaagttgttcgaaaactctggaattttacacggactagtgg
tcatttgctgggatattcaaaacatttgctcgacatatgtgatgtgatgcttctccaatg
cccaatttcgtcaactgttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGAGCTTCTTGTGTGTTATGGCCA 294 603 399 Antisense
GTTATGGCCATTAAGCTATTCGCTA 173 447 414 Antisense
GGGCACGAAGCACATTATTCTTTTC 354 493 581 Antisense
TTTTCAATTCTCCGTCTGACATCAG 145 677 601 Antisense
ACGGAGACTTTGTTGGGCATCGGTG 140 97 702 Antisense
ATCAGTTTGTTATCTACTCCCTGCA 613 27 732 Antisense
TTGCAGAGGTCATCTTCAAACCCAT 511 699 758 Antisense
ATTTCAGTTCGAATACTTCCTGGTC 45 15 805 Antisense
GGACTAGTGGTCATTTGCTGGGATA 461 525 869 Antisense
TGTGATGTGATGCTTCTCCAATGCC 604 555 916 Antisense
CAATGCCCAATTTCGTCAACTGTTT 132 183 934 Antisense
Sequence Source Affymetrix Proprietary Database