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[193421_at]

Description M153.1_rc /REP_DB=WormBase Gene ID /WP=CE03511 /TR=Q21544 /GB=CAA91943.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /REVCOMP /DEF=pyrroline-5-carboxylate reductase
Cluster 6grp_total > 61      4grp_total > 62      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 39.9
(-0.3)
A 		14.7
(-3)
A 		70.5
(3)
A 		15
(-3)
A 		56
B 62.9
(0.7)
A 		80.3
(3)
A 		35.3
(-3)
A 		43.7
(-1.9)
A 		45
C 69.8
(3)
A 		36.5
(-3)
A 		55.1
(0.4)
P 		49.9
(-0.6)
A 		33
avr 57.5
(3) 		43.8
(-0.9) 		53.6
(1.9) 		36.2
(-3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 10.7
(-3)
A 		38.3
(-0.8)
A 		86
(3)
A 		58
(0.8)
A 		42.1
(-0.5)
A 		38
(-0.8)
A 		75
B 42.6
(1.3)
A 		26.2
(-0.7)
A 		30.7
(-0.2)
A 		56.9
(3)
P 		15.1
(-2)
A 		7
(-3)
A 		50
C 37.2
(-3)
A 		41.5
(-2.4)
A 		47.9
(-1.4)
A 		78.3
(3)
A 		51.1
(-1)
P 		41
(-2.4)
A 		41
avr 30.2
(-2.7) 		35.3
(-1.9) 		54.9
(1.4) 		64.4
(3) 		36.1
(-1.8) 		28.7
(-3) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 14.5
(1.6)
A 		3.2
(-2.6)
A 		18.4
(3)
A 		2.2
(-3)
A 		16
B 20.1
(3)
A 		19.8
(2.8)
A 		8.2
(-3)
A 		9.3
(-2.4)
A 		12
C 23.1
(3)
A 		10.5
(-2.1)
A 		19.2
(1.4)
P 		8.4
(-3)
A 		15
avr 19.2
(3) 		11.2
(-0.8) 		15.3
(1.1) 		6.6
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 2
(-3)
A 		13.6
(1.2)
A 		18.7
(3)
A 		14.7
(1.6)
A 		12.7
(0.8)
A 		9.3
(-0.4)
A 		17
B 14.8
(2.9)
A 		8.7
(-0.1)
A 		7.5
(-0.6)
A 		15.1
(3)
P 		6.2
(-1.3)
A 		2.6
(-3)
A 		13
C 13.8
(-0.8)
A 		12.7
(-2.3)
A 		12.2
(-3)
A 		15.7
(1.9)
A 		16.5
(3)
P 		13.7
(-0.9)
A 		4
avr 10.2
(-1.5) 		11.7
(-0.1) 		12.8
(0.9) 		15.2
(3) 		11.8
(-0) 		8.5
(-3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193421_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.28159
Sequence Type Exemplar sequence
Representative Public ID M153.1 NCBI
Target Description M153.1_rc /REP_DB=WormBase Gene ID /WP=CE03511 /TR=Q21544 /GB=CAA91943.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /REVCOMP /DEF=pyrroline-5-carboxylate reductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Design Representative Id based pipeline to a Affymetrix Proprietary Database identifier using 1 transcript(s).
Annotation Grade This is a grade R annotation.
Annotation Transcript Cluster (# of Matching Probes) M153.1
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
M153.1 M153.1_rc /REP_DB=WormBase Gene ID /WP=CE03511 /TR=Q21544 /GB=CAA91943.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /REVCOMP /DEF=pyrroline-5-carboxylate reductase A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_077631 11/11 Negative Strand Matching Probes A 
GENEFINDER00000043670 11/11 Negative Strand Matching Probes None
SNAP00000043667 11/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12146765-12148171(-) 100.0 100.0
Public Domain and Genome References
Ensembl M153.1
Sequence
Target Sequence 
>C. ELEGANS:193421_AT
acttggtccagagccggagagtcctgttactgggtcgatatgaatttctggaacctccac
tgcaaaaccaacagtgctcaagagtttctctacagtttcggcgtcgccatcacgacaagc
agatcccatggcgaacgctgatgcaccggcgcgaacaactgatggggtatttggcatgac
acgtaccactcgagatttcgttgggagaagcgattcaatatttcttatagtgattccaag
tgcaatagaaactaccaaatgttctttgctaagggctggagcgatttcagaagcaacttt
gctcacgtgcactggcttgacggcaagaaacacaacatcacttttttgcacaacttcagc
attatcatgagtagtgttcaaccctagtgccttgcattggtcaaggaacacttcatcgcg
ttttggcgaactggcaataatgttgtctgctgtgattctgcctgagttgatgaggcctct
ggcaagagcctgagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTGGTCCAGAGCCGGAGAGTCCT 676 57 325 Antisense
GAGAGTCCTGTTACTGGGTCGATAT 458 395 341 Antisense
GAATTTCTGGAACCTCCACTGCAAA 122 325 366 Antisense
CAAGAGTTTCTCTACAGTTTCGGCG 519 183 403 Antisense
TCACGACAAGCAGATCCCATGGCGA 204 627 434 Antisense
CCCATGGCGAACGCTGATGCACCGG 563 257 449 Antisense
GCATGACACGTACCACTCGAGATTT 608 309 498 Antisense
AACCCTAGTGCCTTGCATTGGTCAA 706 145 704 Antisense
AAGGAACACTTCATCGCGTTTTGGC 678 159 727 Antisense
TAATGTTGTCTGCTGTGATTCTGCC 28 637 762 Antisense
TGAGGCCTCTGGCAAGAGCCTGAGC 455 567 795 Antisense
Sequence Source Affymetrix Proprietary Database