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[193418_s_at] glutathione S-transferase

Description R05F9.5B /REP_DB=WormBase Gene ID /WP=CE04808 /TR=Q21744 /GB=AAA83166.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glutathione s-transferase and s-crystallin
Cluster 6grp_high1_10_10 > 01      6grp_total > 08      4grp_total > 52      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 8
(-3)
A 		64.3
(3)
A 		39.3
(0.3)
A 		23.7
(-1.3)
A 		56
B 93.3
(3)
A 		29.9
(-1.3)
A 		20.5
(-2)
A 		5.1
(-3)
A 		88
C 20.8
(0.2)
A 		18.7
(-0.3)
A 		33.3
(3)
A 		6.8
(-3)
A 		27
avr 40.7
(3) 		37.6
(2.4) 		31
(1) 		11.9
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 156.5
(3)
P 		60.9
(-2.6)
P 		99.9
(-0.3)
P 		72
(-2)
P 		56.3
(-2.9)
P 		54.1
(-3)
A 		102
B 98.2
(1.5)
P 		92.6
(1.1)
P 		120.6
(3)
P 		82.9
(0.4)
P 		56.3
(-1.4)
P 		32.9
(-3)
A 		88
C 152.6
(3)
P 		77.3
(-1.3)
P 		65.7
(-2)
M 		76.3
(-1.4)
P 		52.9
(-2.7)
P 		47.9
(-3)
P 		105
avr 135.8
(3) 		76.9
(-0.9) 		95.4
(0.3) 		77.1
(-0.9) 		55.2
(-2.3) 		45
(-3) 		91
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 2.9
(-3)
A 		14
(3)
A 		10.3
(1)
A 		3.5
(-2.7)
A 		11
B 29.8
(3)
A 		7.4
(-1.7)
A 		4.7
(-2.2)
A 		1.1
(-3)
A 		29
C 6.9
(0.3)
A 		5.4
(-0.6)
A 		11.6
(3)
A 		1.2
(-3)
A 		10
avr 13.2
(3) 		8.9
(0.7) 		8.9
(0.7) 		1.9
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 28.9
(3)
P 		21.7
(0.2)
P 		21.7
(0.2)
P 		18.2
(-1.1)
P 		17
(-1.5)
P 		13.2
(-3)
A 		16
B 34.2
(3)
P 		30.8
(2.1)
P 		29.3
(1.7)
P 		22
(-0.3)
P 		22.9
(-0.1)
P 		12.3
(-3)
A 		22
C 56.6
(3)
P 		23.6
(-1.8)
P 		16.7
(-2.8)
M 		15.3
(-3)
P 		17.1
(-2.7)
P 		16
(-2.9)
P 		41
avr 39.9
(3) 		25.4
(-0.3) 		22.6
(-1) 		18.5
(-1.9) 		19
(-1.8) 		13.8
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193418_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5190
Sequence Type Exemplar sequence
Representative Public ID R05F9.5B NCBI
Target Description R05F9.5B /REP_DB=WormBase Gene ID /WP=CE04808 /TR=Q21744 /GB=AAA83166.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glutathione s-transferase and s-crystallin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027095(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027095 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-9) (gst-9) mRNA, complete cds. 11/11 None
SNAP00000043867 ENSEMBL cdna:SNAP chromosome:CEL140:II:4891574:4893471:1 11/11 None
R05F9.5a ENSEMBL cdna:known chromosome:CEL140:II:4891574:4893471:1 gene:R05F9.5 11/11 None
R05F9.5b ENSEMBL cdna:known chromosome:CEL140:II:4892784:4893471:1 gene:R05F9.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000043886 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4892781-4893469(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-9
Ensembl R05F9.5
Entrez Gene 187615 Entrez gene
SwissProt Q21743 EMBL-EBI
WormBase CE04807 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001022266 Glutathione S-Transferase family member (gst-9) [Caenorhabditis elegans] gb|AAA83164.1| Glutathione s-transferase protein 9 [Caenorhabditis elegans] sp|Q21743|GST9_CAEEL Probable glutathione S-transferase 9 (GST class-sigma) 1.0E-114
blast CAE58995 Hypothetical protein CBG02268 [Caenorhabditis briggsae] 3.0E-92
blast NP_001022266 Glutathione S-Transferase family member (gst-9) [Caenorhabditis elegans] gb|AAA83164.1| Glutathione s-transferase protein 9 [Caenorhabditis elegans] sp|Q21743|GST9_CAEEL Probable glutathione S-transferase 9 (GST class-sigma) 1.0E-88
blast CAE58992 Hypothetical protein CBG02265 [Caenorhabditis briggsae] emb|CAE56959.1| Hypothetical protein CBG24809 [Caenorhabditis briggsae] 3.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 0.0070
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 7.7E-16
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 0.0070
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 2.4E-7
Sequence
Target Sequence 
>C. ELEGANS:193418_S_AT
tgaccccatttggacaagttccagttctagaagttgatggaagacaactggcacaatcga
ttacaattgttcggtatctatcaaaacaatttggaatttctggaaaatcatcatgggaag
aagctcaagttgatgctctcggtgaccaattcaaggattatcgtgttgaagcacggccat
tctttagggccaaaatgggattttccgatggagatgtagatcaactttacaaagatttat
tcgttccggctttcaataaaatgtactcaattttcactgaatctttgaaatccagtggaa
gtggtttccttgttggagattctttaacttggatggatttggccattgctcaacattcag
ctgatctcttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACCCCATTTGGACAAGTTCCAGT 579 553 47 Antisense
GAAGACAACTGGCACAATCGATTAC 604 339 86 Antisense
GATTACAATTGTTCGGTATCTATCA 53 431 105 Antisense
AAGTTGATGCTCTCGGTGACCAATT 477 155 173 Antisense
GAAGCACGGCCATTCTTTAGGGCCA 431 341 214 Antisense
TTACAAAGATTTATTCGTTCCGGCT 600 681 273 Antisense
ATTCGTTCCGGCTTTCAATAAAATG 149 3 285 Antisense
GTACTCAATTTTCACTGAATCTTTG 283 459 309 Antisense
GGAGATTCTTTAACTTGGATGGATT 34 519 361 Antisense
GGATTTGGCCATTGCTCAACATTCA 31 511 381 Antisense
GCTCAACATTCAGCTGATCTCTTGG 112 301 394 Antisense
Sequence Source Affymetrix Proprietary Database