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[193405_at] GTPase activating protein (GAP)

Description T24C12.2 /REP_DB=WormBase Gene ID /WP=CE05002 /GEN=gap-1 /TR=Q22720 /GB=AAA82387.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=GTPase activating protein (GAP)
Cluster 6grp_total > 32      4grp_total > 54      6grp_cgc > 80      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 19.2
(3)
A 		11.9
(0.3)
A 		2.9
(-3)
A 		16.5
(2)
A 		16
B 5.8
(-3)
A 		23.8
(3)
A 		6.3
(-2.8)
A 		6.5
(-2.8)
A 		18
C 36.8
(2.3)
A 		14.1
(-3)
A 		39.8
(3)
A 		21.1
(-1.4)
A 		26
avr 20.6
(3) 		16.6
(-1.1) 		16.3
(-1.4) 		14.7
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 7.4
(-1.7)
A 		11.8
(-0.5)
A 		24.7
(3)
A 		7.3
(-1.7)
A 		8.6
(-1.4)
A 		2.7
(-3)
A 		22
B 6.7
(0.4)
A 		6.1
(-0.1)
A 		5
(-1.1)
A 		2.8
(-3)
A 		9.7
(3)
A 		3.2
(-2.7)
A 		7
C 15
(3)
A 		9.4
(-0.6)
A 		5.6
(-3)
A 		11.6
(0.8)
A 		7.2
(-2)
A 		6.7
(-2.3)
A 		9
avr 9.7
(1.3) 		9.1
(0.9) 		11.8
(3) 		7.2
(-0.6) 		8.5
(0.4) 		4.2
(-3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 7
(3)
A 		2.6
(-1.2)
A 		0.7
(-3)
A 		2.4
(-1.4)
A 		6
B 1.8
(-2.5)
A 		5.9
(3)
A 		1.5
(-2.9)
A 		1.4
(-3)
A 		5
C 12.2
(2)
A 		4
(-2.8)
A 		13.9
(3)
A 		3.6
(-3)
A 		10
avr 7
(3) 		4.2
(-0.7) 		5.4
(0.9) 		2.5
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 1.4
(-2.1)
A 		4.2
(1.5)
A 		5.4
(3)
A 		1.8
(-1.6)
A 		2.6
(-0.6)
A 		0.7
(-3)
A 		5
B 2.3
(-0)
A 		2
(-0.6)
A 		1.2
(-2.1)
A 		0.7
(-3)
A 		3.9
(3)
A 		1.2
(-2.1)
A 		3
C 5.5
(3)
A 		2.9
(-0.8)
A 		1.4
(-3)
A 		2.3
(-1.7)
A 		2.3
(-1.7)
A 		2.2
(-1.8)
A 		4
avr 3.1
(3) 		3
(2.6) 		2.7
(1.6) 		1.6
(-2.3) 		2.9
(2.3) 		1.4
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193405_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18154
Sequence Type Exemplar sequence
Representative Public ID T24C12.2 NCBI
Target Description T24C12.2 /REP_DB=WormBase Gene ID /WP=CE05002 /GEN=gap-1 /TR=Q22720 /GB=AAA82387.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=GTPase activating protein (GAP)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076003(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076003 NCBI Caenorhabditis elegans GTPase Activating Protein family member (gap-1) (gap-1) mRNA, complete cds. 11/11 None
SNAP00000041013 ENSEMBL cdna:SNAP chromosome:CEL140:X:2192497:2195379:1 11/11 None
GENEFINDER00000041018 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2192497:2195379:1 11/11 None
T24C12.2 ENSEMBL cdna:known chromosome:CEL140:X:2192497:2195341:1 gene:T24C12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2192496-2195341(+) 100.0 100.0
Public Domain and Genome References
Gene Title GTPase activating protein (GAP)
Gene Symbol gap-1
Ensembl T24C12.2
Entrez Gene 180530 Entrez gene
SwissProt Q22720 EMBL-EBI
WormBase CE05002 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604509_AT similar to RAS p21 protein activator 3 cfa
CANINE_2:CFA.539.1.S1_AT similar to RAS p21 protein activator 3 cfa
CANINE_2:CFAAFFX.10680.1.S1_S_AT similar to RAS p21 protein activator 3 cfa
DROSOPHILA_2:1635325_A_AT GTPase-activating protein 1 dm
DROSOPHILA_2:1629842_AT GTPase-activating protein 1 dm
DROSGENOME1:151669_AT GTPase-activating protein 1 dm
DROSGENOME1:143456_AT GTPase-activating protein 1 dm
CHICKEN:GGA.16487.1.S1_S_AT RAS p21 protein activator 3 gga
CHICKEN:GGA.9999.1.S1_AT RAS p21 protein activator 3 gga
CHICKEN:GGAAFFX.25081.1.S1_S_AT RAS p21 protein activator 3 gga
HG-U133_PLUS_2:206220_S_AT RAS p21 protein activator 3 hs
HG-U133A_2:206220_S_AT RAS p21 protein activator 3 hs
HG-U133A:206220_S_AT RAS p21 protein activator 3 hs
HG-FOCUS:206220_S_AT RAS p21 protein activator 3 hs
HG-U133_PLUS_2:206221_AT RAS p21 protein activator 3 hs
HG-U133A:206221_AT RAS p21 protein activator 3 hs
HG-U133A_2:206221_AT RAS p21 protein activator 3 hs
HG-U133_PLUS_2:225562_AT RAS p21 protein activator 3 hs
HG-U133B:225562_AT RAS p21 protein activator 3 hs
HG-U95AV2:32862_AT RAS p21 protein activator 3 hs
U133_X3P:G12545409_3P_A_AT RAS p21 protein activator 3 hs
U133_X3P:HS.119274.1.S1_3P_AT RAS p21 protein activator 3 hs
HG-U95E:78379_AT RAS p21 protein activator 3 hs
HG-U95B:42902_AT RAS p21 protein activator 3 hs
HG-U95E:68664_AT RAS p21 protein activator 3 hs
HG-U95D:73200_AT RAS p21 protein activator 3 hs
HU35KSUBB:RC_R17059_AT RAS p21 protein activator 3 hs
MU11KSUBA:U20238_S_AT RAS p21 protein activator 3 mm
MG-U74AV2:93319_AT RAS p21 protein activator 3 mm
MOE430A:1415850_AT RAS p21 protein activator 3 mm
MOUSE430A_2:1415850_AT RAS p21 protein activator 3 mm
MOUSE430_2:1415850_AT RAS p21 protein activator 3 mm
MU19KSUBA:TC21840_AT RAS p21 protein activator 3 (Rasa3), mRNA mm
RG-U34C:RC_AI237779_AT RAS p21 protein activator 3 rn
RG-U34C:RC_AI228998_F_AT RAS p21 protein activator 3 rn
RG-U34C:RC_AI170661_AT RAS p21 protein activator 3 rn
RAE230A:1370942_AT RAS p21 protein activator 3 rn
RAT230_2:1370942_AT RAS p21 protein activator 3 rn
RAE230B:1392224_AT RAS p21 protein activator 3 rn
RAT230_2:1392224_AT RAS p21 protein activator 3 rn
RG-U34B:RC_AA924049_AT RAS p21 protein activator 3 rn
Protein Similarities
Method ID Description E-Value
blast AAA82387 Gtpase activating protein family protein 1 [Caenorhabditis elegans] ref|NP_508404.1| GTPase Activating Protein family member (gap-1) [Caenorhabditis elegans] gb|AAD13781.1| GTPase activating protein GAP-1 [Caenorhabditis elegans] sp|Q22720|GAP1_CAEEL Ras GTPase-activating protein gap-1 (GTPase-activating protein 1) 0.0
blast CAE68342 Hypothetical protein CBG14068 [Caenorhabditis briggsae] 0.0
blast NP_777101 RAS p21 protein activator (GTPase activating protein) 3 (Ins(1,3,4,5)P4-binding protein) [Bos taurus] sp|Q28013|RASA3_BOVIN Ras GTPase-activating protein 3 (GAP1(IP4BP)) (Ins P4-binding protein) gb|AAC48500.1| R-Ras GTPase activating protein prf||2206412A GTPase-activating protein 2.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR001936 EMBL-EBI Ras GTPase-activating protein 1.3E-32
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.6E-16
Sequence
Target Sequence 
>C. ELEGANS:193405_AT
ttgaccgaagccgcctggcttggaagaaagttctacactacaaaaaacgatatgtgcaat
taacaaacacacatttgatctggcaaaaggatgtgcagtgtgctcctaaaggaacagttc
ctttatcagatatcaagtttgtgaatgttgacaacaaaaacatcataacgattgtctgtg
agactatgcagctgcaatttgaagctcccggaggtgtagaagcaactgactggctgaatg
caatcgagaggcagcgaaatcgtgctgcacacgaaattgcagaaacgcctggagagcact
tcttcgttgacgctgaaagacacgtggataaaattcatactctgctatacaaataccgcg
agacaatgattgaatggagggaccaactgcaatcaaacgtcgagttggacgagaaaacgg
cgccggaactgctaaaagccagctatgtcgttgaagaagagcgacaatcgcataaagact
cacttattgcgaccctctgttccacgatcgacgtcacggatgccattcagttagcacata
cggagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGACCGAAGCCGCCTGGCTTGGAA 9 681 1274 Antisense
GGAACAGTTCCTTTATCAGATATCA 343 529 1384 Antisense
GAAGCTCCCGGAGGTGTAGAAGCAA 284 343 1474 Antisense
AGAAACGCCTGGAGAGCACTTCTTC 209 73 1554 Antisense
AGCACTTCTTCGTTGACGCTGAAAG 448 77 1568 Antisense
CATACTCTGCTATACAAATACCGCG 337 213 1609 Antisense
GGAGGGACCAACTGCAATCAAACGT 271 239 1649 Antisense
GGCGCCGGAACTGCTAAAAGCCAGC 606 533 1692 Antisense
TAAAGACTCACTTATTGCGACCCTC 527 641 1746 Antisense
CACGATCGACGTCACGGATGCCATT 628 199 1776 Antisense
GCCATTCAGTTAGCACATACGGAGT 478 279 1795 Antisense
Sequence Source Affymetrix Proprietary Database