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[193402_at]

Description Y59A8B.14 /REP_DB=WormBase Gene ID /WP=CE27410 /GEN=par-4 /TR=Q9GN62 /GB=CAC14418.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine protein kinase
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 79      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 91      6grp_total > 59      4grp_total > 22      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 246.2
(3)
P 		62.1
(-3)
P 		92.7
(-2)
P 		203
(1.6)
P 		184
B 163.7
(3)
P 		96.9
(-1.1)
P 		66.9
(-3)
P 		155.5
(2.5)
P 		97
C 157.5
(0.2)
P 		114.1
(-2.2)
P 		99.8
(-3)
P 		207.7
(3)
P 		108
avr 189.1
(3) 		91
(-2.7) 		86.5
(-3) 		188.7
(3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 223.7
(1.6)
P 		129.4
(-3)
P 		184.7
(-0.3)
P 		143
(-2.3)
P 		216.1
(1.2)
P 		252.6
(3)
P 		123
B 208
(-1.1)
P 		160.1
(-2.6)
P 		148.2
(-3)
P 		184.2
(-1.8)
P 		211.4
(-1)
P 		334
(3)
P 		186
C 276.1
(3)
P 		184.2
(-1.9)
P 		164.7
(-3)
P 		165.5
(-3)
P 		237.3
(0.9)
P 		198.4
(-1.2)
P 		111
avr 235.9
(1.5) 		157.9
(-3) 		165.9
(-2.5) 		164.2
(-2.6) 		221.6
(0.7) 		261.7
(3) 		104
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 89.6
(3)
P 		13.5
(-3)
P 		24.2
(-2.2)
P 		29.7
(-1.7)
P 		76
B 52.2
(3)
P 		23.9
(-1.6)
P 		15.5
(-3)
P 		33.1
(-0.1)
P 		37
C 52.1
(3)
P 		32.8
(-3)
P 		34.8
(-2.4)
P 		35
(-2.3)
P 		19
avr 64.6
(3) 		23.4
(-3) 		24.8
(-2.8) 		32.6
(-1.7) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 41.2
(-2)
P 		46
(-1)
P 		40.2
(-2.2)
P 		36.3
(-3)
P 		65.2
(3)
P 		61.5
(2.2)
P 		29
B 72.4
(-0.5)
P 		53.3
(-1.8)
P 		36
(-3)
P 		48.8
(-2.1)
P 		85.9
(0.4)
P 		124.5
(3)
P 		89
C 102.3
(3)
P 		56.2
(-1)
P 		41.9
(-2.3)
P 		33.3
(-3)
P 		76.8
(0.8)
P 		66.4
(-0.1)
P 		69
avr 72
(1.4) 		51.8
(-1.3) 		39.4
(-3) 		39.5
(-3) 		76
(1.9) 		84.1
(3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193402_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13601
Sequence Type Exemplar sequence
Representative Public ID Y59A8B.14 NCBI
Target Description Y59A8B.14 /REP_DB=WormBase Gene ID /WP=CE27410 /GEN=par-4 /TR=Q9GN62 /GB=CAC14418.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075132
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000043026 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:18106796:18110152:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18101260-18110097(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC14418 Hypothetical protein Y59A8B.14 [Caenorhabditis elegans] emb|CAC15862.2| Hypothetical protein Y59A8B.14 [Caenorhabditis elegans] ref|NP_507533.1| abnormal embryonic PARtitioning of cytoplasm family member (par-4) [Caenorhabditis elegans] gb|AAD45355.1| putative serine-threonine kinase PAR-4 [Caenorhabditis elegans] 1.0E-113
blast CAE61699 Hypothetical protein CBG05647 [Caenorhabditis briggsae] 3.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-14
Sequence
Target Sequence 
>C. ELEGANS:193402_AT
gttctcgtaccgatacatattttattgattgctcaagcttctttgtcttttctaaccgat
taacactgtttgtgtgtagtaaacgtgctctatcgccaccttttctcagcaacaaaaaaa
aacttaattttccggcagtttctcgattttttttcggttttttttcgttagaaacccccc
aaattcgtcatatctttatttttttgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCTCGTACCGATACATATTTTAT 582 443 1742 Antisense
GATACATATTTTATTGATTGCTCAA 538 423 1753 Antisense
ATTGCTCAAGCTTCTTTGTCTTTTC 179 7 1769 Antisense
TCAAGCTTCTTTGTCTTTTCTAACC 49 633 1774 Antisense
GTCTTTTCTAACCGATTAACACTGT 664 467 1786 Antisense
AACCGATTAACACTGTTTGTGTGTA 445 147 1795 Antisense
GTGTAGTAAACGTGCTCTATCGCCA 371 485 1815 Antisense
TAATTTTCCGGCAGTTTCTCGATTT 191 633 1866 Antisense
TAGAAACCCCCCAAATTCGTCATAT 132 639 1910 Antisense
CCCCCCAAATTCGTCATATCTTTAT 251 261 1916 Antisense
TCGTCATATCTTTATTTTTTTGCTC 274 605 1926 Antisense
Sequence Source Affymetrix Proprietary Database