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[193400_s_at]

Description B0464.5B /REP_DB=WormBase Gene ID /WP=CE20457 /GEN=srk-1 /TR=Q9XVY0 /GB=CAA79541.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 60      6grp_total > 17      4grp_total > 37      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 282
1 2 3 4 range
A 545.3
(2.2)
P 		428
(-0.3)
P 		302.9
(-3)
P 		584.4
(3)
P 		282
B 522.3
(3)
P 		444
(0.9)
P 		378.7
(-0.9)
P 		303.6
(-3)
P 		219
C 553.4
(3)
P 		502.2
(1.3)
P 		374.7
(-3)
P 		447.1
(-0.6)
P 		179
avr 540.3
(3) 		458.1
(0.4) 		352.1
(-3) 		445
(-0) 		188

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 482
1 2 3 4 5 6 range
A 675.2
(3)
P 		507.8
(0.6)
P 		318.5
(-2.2)
P 		260.8
(-3)
P 		363
(-1.5)
P 		430.2
(-0.5)
P 		414
B 596.7
(3)
P 		358.3
(-0.5)
P 		295.9
(-1.5)
P 		350.1
(-0.7)
P 		193.7
(-3)
P 		311.8
(-1.2)
P 		403
C 651.8
(3)
P 		492
(0.5)
P 		340.3
(-1.9)
P 		281
(-2.8)
P 		266.6
(-3)
P 		297.5
(-2.5)
P 		385
avr 641.2
(3) 		452.7
(-0.1) 		318.2
(-2.3) 		297.3
(-2.6) 		274.4
(-3) 		346.5
(-1.8) 		367
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 198.5
(3)
P 		92.9
(-2.3)
P 		79.2
(-3)
P 		85.5
(-2.7)
P 		119
B 166.6
(3)
P 		109.7
(-0.4)
P 		87.8
(-1.6)
P 		64.7
(-3)
P 		102
C 183.2
(3)
P 		144.4
(0.8)
P 		130.6
(0.1)
P 		75.4
(-3)
P 		108
avr 182.8
(3) 		115.7
(-0.7) 		99.2
(-1.7) 		75.2
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 124.5
(0.1)
P 		180.6
(3)
P 		69.4
(-2.8)
P 		66.1
(-3)
P 		109.5
(-0.7)
P 		104.7
(-1)
P 		115
B 207.6
(3)
P 		119.3
(-0.9)
P 		71.9
(-3)
P 		92.7
(-2.1)
P 		78.7
(-2.7)
P 		116.3
(-1)
P 		136
C 241.6
(3)
P 		150.1
(0)
P 		86.6
(-2)
P 		56.5
(-3)
P 		86.3
(-2)
P 		99.6
(-1.6)
P 		185
avr 191.2
(3) 		150
(0.9) 		76
(-2.8) 		71.8
(-3) 		91.5
(-2) 		106.9
(-1.2) 		119
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193400_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6255
Sequence Type Exemplar sequence
Representative Public ID B0464.5B NCBI
Target Description B0464.5B /REP_DB=WormBase Gene ID /WP=CE20457 /GEN=srk-1 /TR=Q9XVY0 /GB=CAA79541.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025962(11), NM_001025963(11), NM_066679(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025962 NCBI Caenorhabditis elegans SR Protein Kinase family member (spk-1) (spk-1) mRNA, complete cds. 11/11 None
NM_001025963 NCBI Caenorhabditis elegans SR Protein Kinase family member (spk-1) (spk-1) mRNA, complete cds. 11/11 None
NM_066679 NCBI Caenorhabditis elegans SR Protein Kinase family member (spk-1) (spk-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000014960 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9456538:9462156:1 11/11 None
SNAP00000014943 ENSEMBL cdna:SNAP chromosome:CEL140:III:9457880:9462156:1 11/11 None
B0464.5b.2 ENSEMBL cdna:known chromosome:CEL140:III:9456459:9462883:1 gene:B0464.5 11/11 None
B0464.5b.1 ENSEMBL cdna:known chromosome:CEL140:III:9456510:9462855:1 gene:B0464.5 11/11 None
B0464.5c.1 ENSEMBL cdna:known chromosome:CEL140:III:9456538:9462869:1 gene:B0464.5 11/11 A 
B0464.5c.2 ENSEMBL cdna:known chromosome:CEL140:III:9456538:9462869:1 gene:B0464.5 11/11 None
B0464.5a ENSEMBL cdna:known chromosome:CEL140:III:9457550:9462156:1 gene:B0464.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9456533-9462152(+) 97.9 100.0
Public Domain and Genome References
Ensembl B0464.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA79541 Hypothetical protein B0464.5b [Caenorhabditis elegans] ref|NP_001021133.1| SR Protein Kinase family member (spk-1) [Caenorhabditis elegans] gb|AAG36873.1| SR protein specfic kinase SPK-1 [Caenorhabditis elegans] 0.0
blast CAA79540 Hypothetical protein B0464.5a [Caenorhabditis elegans] ref|NP_499080.3| SR Protein Kinase family member (spk-1) [Caenorhabditis elegans] sp|Q03563|SPK1_CAEEL Serine/threonine-protein kinase spk-1 0.0
blast CAA79542 Hypothetical protein B0464.5c [Caenorhabditis elegans] ref|NP_001021134.1| SR Protein Kinase family member (spk-1) [Caenorhabditis elegans] 0.0
blast Q61IS6 Serine/threonine-protein kinase spk-1 emb|CAE65212.1| Hypothetical protein CBG10087 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-28
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-28
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-28
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Pfam IPR000719 EMBL-EBI Protein kinase 5.4E-22
Sequence
Target Sequence 
>C. ELEGANS:193400_S_AT
ggatacggacctccagctgatatttggagtaccgcgtgtatggcttttgaattggcaacc
ggtgactatttgtttgaaccacaccaaggagataactattcacgcgatgaagatcatctt
gcgcacatttccgaacttcttggagcaattccaccgtcaatttacaaaaagggtaaacat
tggagagagttcttccacaaaaatggacacttacttcacattcaccaacttaaaccatgg
tcactatacgaggttttgagacaaaaatacgagtggtctcatgaagacgcgcagcagttt
gaaagtttccttcgtccgatgcttgatttcgatcaggagaaacgatctacggccaaaatt
gcattgaaacatccgttcttgttaccatttggaggaagagcgccaaaatctgattgtcca
cctgagcttctgagcaagatgtttccggatggattgattcccgagccgttcgacggaaat
gagcatcaggaagtttaccgtgacgaaaatgatagtagatcggcgagcgagaggagtgcc
aatagcaggtctgcggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATACGGACCTCCAGCTGATATTT 171 509 1480 Antisense
TTGGAGTACCGCGTGTATGGCTTTT 452 707 1503 Antisense
TGAAGATCATCTTGCGCACATTTCC 467 575 1587 Antisense
GCACATTTCCGAACTTCTTGGAGCA 399 317 1602 Antisense
TGGAGCAATTCCACCGTCAATTTAC 387 547 1620 Antisense
TGGACACTTACTTCACATTCACCAA 64 549 1683 Antisense
TTTCCTTCGTCCGATGCTTGATTTC 538 669 1785 Antisense
GAAACATCCGTTCTTGTTACCATTT 241 357 1845 Antisense
AAAATCTGATTGTCCACCTGAGCTT 627 133 1884 Antisense
GATTCCCGAGCCGTTCGACGGAAAT 645 431 1935 Antisense
GGAGTGCCAATAGCAGGTCTGCGGG 485 517 2012 Antisense
Sequence Source Affymetrix Proprietary Database