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[193388_s_at] PH (pleckstrin homology) domain (2 domains)

Description F10G8.8 /REP_DB=WormBase Gene ID /WP=CE23641 /TR=O62399 /GB=CAA19441.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain (2 domains)
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 91      6grp_total > 80      4grp_total > 47      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 224.8
(3)
P 		64.6
(-3)
P 		74.1
(-2.6)
P 		114.5
(-1.1)
P 		160
B 199.8
(3)
P 		125.3
(-1.6)
P 		101.8
(-3)
P 		113.6
(-2.3)
P 		98
C 141.4
(3)
P 		77.2
(-3)
P 		131.1
(2)
P 		94.5
(-1.4)
P 		64
avr 188.7
(3) 		89
(-3) 		102.3
(-2.2) 		107.5
(-1.9) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 127.7
(-3)
P 		247.8
(1.2)
P 		170.1
(-1.5)
P 		299.7
(3)
P 		219
(0.2)
P 		174.8
(-1.4)
P 		172
B 171.3
(-2.9)
P 		184.1
(-2.2)
P 		202.1
(-1.2)
P 		279.9
(3)
P 		219
(-0.3)
P 		169.4
(-3)
P 		111
C 206.6
(-1.7)
P 		223.2
(-1.3)
P 		260.9
(-0.4)
P 		396
(3)
P 		214
(-1.6)
P 		156.4
(-3)
P 		240
avr 168.5
(-2.9) 		218.4
(-1) 		211
(-1.3) 		325.2
(3) 		217.3
(-1.1) 		166.9
(-3) 		158
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 81.8
(3)
P 		14
(-3)
P 		19.4
(-2.5)
P 		16.8
(-2.8)
P 		68
B 63.7
(3)
P 		30.9
(-1.9)
P 		23.6
(-3)
P 		24.2
(-2.9)
P 		40
C 46.8
(3)
P 		22.2
(-1.8)
P 		45.7
(2.8)
P 		15.9
(-3)
P 		31
avr 64.1
(3) 		22.4
(-2.5) 		29.6
(-1.6) 		19
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 23.5
(-3)
P 		88.1
(3)
P 		37.1
(-1.7)
P 		76
(1.9)
P 		66.1
(1)
P 		42.6
(-1.2)
P 		65
B 59.6
(-1.4)
P 		61.3
(-1.2)
P 		49.1
(-3)
P 		74.1
(0.8)
P 		89
(3)
P 		63.2
(-0.9)
P 		40
C 76.6
(2.3)
P 		68.1
(0.5)
P 		66.4
(0.1)
P 		79.6
(3)
P 		69.2
(0.7)
P 		52.3
(-3)
P 		27
avr 53.2
(-2.5) 		72.5
(2) 		50.9
(-3) 		76.6
(3) 		74.8
(2.6) 		52.7
(-2.6) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193388_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1092
Sequence Type Exemplar sequence
Representative Public ID F10G8.8 NCBI
Target Description F10G8.8 /REP_DB=WormBase Gene ID /WP=CE23641 /TR=O62399 /GB=CAA19441.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060254
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000044083 ENSEMBL cdna:SNAP chromosome:CEL140:I:10061551:10065036:-1 None
GENEFINDER00000044084 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10062452:10065036:-1 None
NM_060254 NCBI Caenorhabditis elegans F10G8.8 (F10G8.8) mRNA, complete cds. None
F10G8.8 ENSEMBL cdna:known chromosome:CEL140:I:10058815:10070129:-1 gene:F10G8.8 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10058976-10070039(-) 96.35 100.0
Public Domain and Genome References
Gene Title PH (pleckstrin homology) domain (2 domains)
Ensembl F10G8.8
Entrez Gene 172870 Entrez gene
SwissProt O62399 EMBL-EBI
WormBase CE30751 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1627054_AT outspread dm
DROSGENOME1:153803_AT outspread dm
Protein Similarities
Method ID Description E-Value
blast CAB02286 Hypothetical protein F10G8.8 [Caenorhabditis elegans] emb|CAA19441.4| Hypothetical protein F10G8.8 [Caenorhabditis elegans] ref|NP_492655.2| F10G8.8 [Caenorhabditis elegans] 0.0
blast CAE60221 Hypothetical protein CBG03790 [Caenorhabditis briggsae] 0.0
blast AAH73109 LOC443626 protein [Xenopus laevis] 5.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.2E-8
Pfam IPR001849 EMBL-EBI Pleckstrin-like 7.6E-14
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.2E-8
Pfam IPR001849 EMBL-EBI Pleckstrin-like 7.6E-14
Sequence
Target Sequence 
>C. ELEGANS:193388_S_AT
tcaatgtatgtgctctctattctgcaaaatgcttggaaaattctcaactcgacgagcaac
tttcaaacttgatcagcgaaaaggaagccagtgttgaattatcgatcgaaaacgacaaac
ttcgatcggaaattgatagaaaatcgaaagaaattggagatcttcaaagaaaaatgaaca
attatgagaaattgagtcagtcgagaacagaccccgaagtgttagccggtacgacagtct
cgcgcgctcgtcgagaaaagcagaagaaaagaaatcgtcgccatgattttaatgttctct
cgtttgtaccaaacctagataaaacatatgaaaaatcgatgaaaaatcactgtcacaata
agtttgagattacagtaccctttgaaggcgcacacatttttgcattttacaaaaaattgt
cgtgtcgagaccggatgttcgtttccacagtaatcccgttgaaattccacaaaaccttat
cgaagacgcccgtcgagctctcgatgttccagtttctgaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAATGTATGTGCTCTCTATTCTGC 434 633 2549 Antisense
AACTCGACGAGCAACTTTCAAACTT 76 141 2594 Antisense
GAGTCAGTCGAGAACAGACCCCGAA 151 399 2742 Antisense
AAGTGTTAGCCGGTACGACAGTCTC 310 155 2765 Antisense
GTCTCGCGCGCTCGTCGAGAAAAGC 366 471 2785 Antisense
TTTAATGTTCTCTCGTTTGTACCAA 321 673 2836 Antisense
CAGTACCCTTTGAAGGCGCACACAT 697 205 2921 Antisense
GTCGAGACCGGATGTTCGTTTCCAC 486 473 2972 Antisense
TCGTTTCCACAGTAATCCCGTTGAA 570 603 2987 Antisense
CACAAAACCTTATCGAAGACGCCCG 257 191 3016 Antisense
GCTCTCGATGTTCCAGTTTCTGAAC 5 301 3045 Antisense
Sequence Source Affymetrix Proprietary Database