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[193383_at]

Description F36H1.4 /REP_DB=WormBase Gene ID /WP=CE28021 /GEN=lin-3 /TR=SW:Q03345 /GB=CAA21649.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=lin-3 growth factor precursor
Cluster 6grp_high1_10_10 > 49      6grp_total > 49      4grp_total > 29      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 245
1 2 3 4 range
A 375.4
(3)
P 		211.6
(-1)
A 		130.1
(-3)
A 		266.9
(0.3)
P 		245
B 248.2
(0.5)
P 		251.1
(0.6)
P 		169.5
(-3)
A 		304.4
(3)
M 		135
C 303.6
(3)
P 		238.9
(0.4)
P 		151.4
(-3)
A 		221.6
(-0.2)
A 		152
avr 309.1
(3) 		233.9
(0.2) 		150.3
(-3) 		264.3
(1.3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 217
1 2 3 4 5 6 range
A 279
(1.9)
P 		238.1
(0.6)
P 		156.8
(-2)
M 		125.8
(-3)
A 		216.7
(-0.1)
P 		311.8
(3)
P 		186
B 226.3
(0.5)
P 		213.5
(-0.1)
P 		145.5
(-3)
P 		171.1
(-1.9)
P 		284
(3)
P 		240.8
(1.1)
P 		139
C 343
(3)
P 		223.1
(-1.1)
P 		171
(-2.9)
M 		168.4
(-3)
A 		211
(-1.5)
P 		276.7
(0.7)
P 		175
avr 282.8
(3) 		224.9
(0.3) 		157.8
(-2.9) 		155.1
(-3) 		237.2
(0.9) 		276.4
(2.7) 		128
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 136.7
(3)
P 		45.9
(-2.3)
A 		34
(-3)
A 		39.1
(-2.7)
P 		103
B 79.1
(3)
P 		62
(0.4)
P 		39.3
(-3)
A 		64.9
(0.9)
M 		40
C 100.5
(3)
P 		68.7
(-0)
P 		52.8
(-1.5)
A 		37.4
(-3)
A 		63
avr 105.4
(3) 		58.9
(-1.4) 		42
(-3) 		47.1
(-2.5) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 51.4
(-0.8)
P 		84.7
(3)
P 		34.1
(-2.8)
M 		31.9
(-3)
A 		65.4
(0.8)
P 		75.9
(2)
P 		53
B 78.7
(0.3)
P 		71.1
(-0.3)
P 		35.3
(-3)
P 		45.3
(-2.3)
P 		115.4
(3)
P 		89.8
(1.1)
P 		80
C 127.1
(3)
P 		68.1
(-0.8)
P 		43.5
(-2.4)
M 		33.9
(-3)
A 		68.3
(-0.8)
P 		92.6
(0.8)
P 		93
avr 85.7
(3) 		74.6
(1.6) 		37.6
(-2.9) 		37
(-3) 		83
(2.6) 		86.1
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193383_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9576
Sequence Type Exemplar sequence
Representative Public ID F36H1.4 NCBI
Target Description F36H1.4 /REP_DB=WormBase Gene ID /WP=CE28021 /GEN=lin-3 /TR=SW:Q03345 /GB=CAA21649.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=lin-3 growth factor precursor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171418(11), NM_171919(11), NM_171918(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171418 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-3) (lin-3) mRNA, complete cds. 11/11 None
NM_171919 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-3) (lin-3) mRNA, complete cds. 11/11 None
NM_171918 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-3) (lin-3) mRNA, complete cds. 11/11 None
F36H1.4a ENSEMBL cdna:known chromosome:CEL140:IV:11054282:11063585:1 gene:F36H1.4 11/11 None
F36H1.4b.1 ENSEMBL cdna:known chromosome:CEL140:IV:11057642:11063143:1 gene:F36H1.4 11/11 None
F36H1.4b.2 ENSEMBL cdna:known chromosome:CEL140:IV:11057759:11063143:1 gene:F36H1.4 11/11 None
F36H1.4c ENSEMBL cdna:known chromosome:CEL140:IV:11057786:11063492:1 gene:F36H1.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001502 5/11 Cross Hyb Matching Probes None
GENEFINDER00000001512 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11057791-11063149(+) 95.12 100.0
Public Domain and Genome References
Ensembl F36H1.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA48207 Lin-3 [Caenorhabditis elegans] emb|CAA92997.2| Hypothetical protein F36H1.4a [Caenorhabditis elegans] emb|CAA21649.2| Hypothetical protein F36H1.4a [Caenorhabditis elegans] ref|NP_741488.1| abnormal cell LINeage family member (lin-3) [Caenorhabditis elegans] sp|Q03345|LIN3_CAEEL Lin-3 protein precursor (Abnormal cell lineage protein 3) prf||1815239A vulval inducing protein 0.0
blast CAD27623 Hypothetical protein F36H1.4b [Caenorhabditis elegans] emb|CAD27613.1| Hypothetical protein F36H1.4b [Caenorhabditis elegans] ref|NP_741490.1| abnormal cell LINeage family member (lin-3) [Caenorhabditis elegans] 0.0
blast CAD30434 Hypothetical protein F36H1.4c [Caenorhabditis elegans] emb|CAD30455.1| Hypothetical protein F36H1.4c [Caenorhabditis elegans] ref|NP_741489.1| abnormal cell LINeage family member (lin-3) [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741488.1 1 231-253
NP_741490.1 1 229-251
NP_741489.1 1 216-238
F36H1.4a 1 231-253
F36H1.4b.1 1 229-251
F36H1.4b.2 1 229-251
F36H1.4c 1 216-238
Sequence
Target Sequence 
>C. ELEGANS:193383_AT
tattcctgcgttcgcatttctcattgtcatgctcatcatgtttataacaattgttgttta
tgcttatagaagaatgtctaaacgatcggatgatatgacatatacaatgagtcatatgtg
cccaccagaagcattcaatgtcctcaaaacaccaaatggacgacatattccagttcatca
aattccatcatgttcttatactatcccaacaccgggtacagtacctccaaatatatcatc
aactcctggatcaagaatacccactcgtcaacaagctattcgaaataatgaacaagcacg
gaacaactttttcagcattctcagaagtcaaggtaccattccatccaggagtatcaatga
cgacgacacgccgaagcactacaagtcagtgccacgtgttgaagtttcagcaattaatta
ctctggccacattgatttttcaacagtatcatatcagtcgactgaatcagaagtttcaaa
agcatcagtaacatgtccaccaccggcgcacactgtaattaatatcgagttggattctgc
agatacgaattttcgatccccgtctcgaagttctggagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTCCTGCGTTCGCATTTCTCATT 386 665 699 Antisense
ATTTCTCATTGTCATGCTCATCATG 499 15 714 Antisense
TCCATCATGTTCTTATACTATCCCA 69 591 882 Antisense
TACTATCCCAACACCGGGTACAGTA 90 625 897 Antisense
TACCCACTCGTCAACAAGCTATTCG 535 649 956 Antisense
GGTACCATTCCATCCAGGAGTATCA 296 505 1030 Antisense
TCAATGACGACGACACGCCGAAGCA 183 633 1052 Antisense
CAGTGCCACGTGTTGAAGTTTCAGC 684 207 1085 Antisense
ATTACTCTGGCCACATTGATTTTTC 115 13 1115 Antisense
AGATACGAATTTTCGATCCCCGTCT 684 63 1239 Antisense
ATCCCCGTCTCGAAGTTCTGGAGAA 239 39 1254 Antisense
Sequence Source Affymetrix Proprietary Database