[Home] | [Total List]

[193378_at] bumetanide-sensitive Na-K-Cl cotransporter

Description F10E7.9 /REP_DB=WormBase Gene ID /WP=CE04364 /TR=Q19301 /GB=AAA82423.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=bumetanide-sensitive Na-K-Cl cotransporter
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 06      4grp_highest_8_8 > 51      4grp_high_8_8 > 25      6grp_highest_nonorm > 11      6grp_total > 14      4grp_total > 39      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1963
1 2 3 4 range
A 2661.2
(3)
P 		731.7
(-2.9)
P 		702.6
(-3)
P 		1179.1
(-1.5)
P 		1959
B 2351.2
(3)
P 		997
(-1.9)
P 		701.5
(-3)
P 		1350.4
(-0.6)
P 		1650
C 2664.4
(3)
P 		938.1
(-2.7)
P 		836
(-3)
P 		1076.2
(-2.2)
P 		1828
avr 2558.9
(3) 		888.9
(-2.5) 		746.7
(-3) 		1201.9
(-1.5) 		1812

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1626
1 2 3 4 5 6 range
A 2204.2
(3)
P 		1730.9
(0.8)
P 		1555.4
(-0.1)
P 		1528.8
(-0.2)
P 		1099.4
(-2.2)
P 		929.1
(-3)
P 		1275
B 2554.6
(3)
P 		2076.5
(1)
P 		1557.2
(-1.1)
P 		1624.8
(-0.9)
P 		1226.9
(-2.5)
P 		1110.4
(-3)
P 		1444
C 2148.5
(3)
P 		2037.6
(2.4)
P 		1382.9
(-1)
P 		1731.4
(0.8)
P 		995.4
(-3)
P 		1118.8
(-2.4)
P 		1153
avr 2302.4
(3) 		1948.3
(1.3) 		1498.5
(-0.9) 		1628.3
(-0.2) 		1107.2
(-2.7) 		1052.8
(-3) 		1250
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 810
1 2 3 4 range
A 969
(3)
P 		158.8
(-3)
P 		183.7
(-2.8)
P 		172.6
(-2.9)
P 		810
B 749.8
(3)
P 		246.2
(-2.1)
P 		162.5
(-3)
P 		287.8
(-1.7)
P 		587
C 882
(3)
P 		269.7
(-2.2)
P 		291.4
(-2.1)
P 		181.5
(-3)
P 		701
avr 866.9
(3) 		224.9
(-2.9) 		212.5
(-3) 		214
(-3) 		654

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 663
1 2 3 4 5 6 range
A 406.3
(-0.2)
P 		615.6
(3)
P 		338.7
(-1.3)
P 		387.7
(-0.5)
P 		331.7
(-1.4)
P 		226.2
(-3)
P 		389
B 888.9
(3)
P 		691.2
(0.7)
P 		378.1
(-3)
P 		430.4
(-2.4)
P 		498.5
(-1.6)
P 		414.1
(-2.6)
P 		511
C 796.3
(3)
P 		621.7
(0.8)
P 		352
(-2.6)
P 		348.2
(-2.7)
P 		322.1
(-3)
P 		374.5
(-2.3)
P 		474
avr 697.2
(3) 		642.8
(2.1) 		356.3
(-2.7) 		388.8
(-2.2) 		384.1
(-2.2) 		338.3
(-3) 		359
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193378_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4436
Sequence Type Exemplar sequence
Representative Public ID F10E7.9 NCBI
Target Description F10E7.9 /REP_DB=WormBase Gene ID /WP=CE04364 /TR=Q19301 /GB=AAA82423.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=bumetanide-sensitive Na-K-Cl cotransporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063068(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063068 NCBI Caenorhabditis elegans F10E7.9 (F10E7.9) mRNA, complete cds. 11/11 None
SNAP00000000727 ENSEMBL cdna:SNAP chromosome:CEL140:II:7106421:7109220:-1 11/11 None
GENEFINDER00000000737 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7106421:7109940:-1 11/11 None
F10E7.9.2 ENSEMBL cdna:known chromosome:CEL140:II:7106341:7109987:-1 gene:F10E7.9 11/11 None
F10E7.9.1 ENSEMBL cdna:known chromosome:CEL140:II:7106341:7109986:-1 gene:F10E7.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7106416-7109936(-) 100.0 100.0
Public Domain and Genome References
Gene Title bumetanide-sensitive Na-K-Cl cotransporter
Ensembl F10E7.9
Entrez Gene 174167 Entrez gene
SwissProt Q19301 EMBL-EBI
WormBase CE04364 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1587182_AT similar to solute carrier family 12, member 8 cfa
CANINE_2:CFA.7290.1.A1_AT similar to solute carrier family 12, member 8 cfa
CANINE_2:CFAAFFX.19471.1.S1_S_AT similar to solute carrier family 12, member 8 cfa
DROSOPHILA_2:1632318_AT dm
DROSGENOME1:144020_AT dm
CHICKEN:GGAAFFX.23760.1.S1_S_AT similar to solute carrier family 12, member 8; solute carrier family 12 (sodium/potassium/chloride transporters), member 8; cation-chloride cotransporter 9 gga
HG-U133_PLUS_2:219874_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U133A:219874_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U133A_2:219874_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
HU35KSUBA:RC_AA291644_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
U133_X3P:G13375853_3P_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U95B:46337_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U95C:62932_R_AT solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U133_PLUS_2:240612_AT Solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U133B:240612_AT Solute carrier family 12 (potassium/chloride transporters), member 8 hs
HG-U95E:69157_AT Solute carrier family 12 (potassium/chloride transporters), member 8 hs
U133_X3P:HS.195264.0.A1_3P_AT Solute carrier family 12 (potassium/chloride transporters), member 8 hs
MG-U74CV2:134291_AT solute carrier family 12 (potassium/chloride transporters), member 8 mm
MOE430A:1420334_AT solute carrier family 12 (potassium/chloride transporters), member 8 mm
MOUSE430A_2:1420334_AT solute carrier family 12 (potassium/chloride transporters), member 8 mm
MOUSE430_2:1420334_AT solute carrier family 12 (potassium/chloride transporters), member 8 mm
MG-U74BV2:105067_AT Solute carrier family 12 (potassium/chloride transporters), member 8, mRNA (cDNA clone MGC:31673 IMAGE:4910321) mm
MOE430B:1458918_AT Solute carrier family 12 (potassium/chloride transporters), member 8, mRNA (cDNA clone MGC:31673 IMAGE:4910321) mm
MOUSE430_2:1458918_AT Solute carrier family 12 (potassium/chloride transporters), member 8, mRNA (cDNA clone MGC:31673 IMAGE:4910321) mm
RG-U34B:RC_AA924742_AT cation-chloride cotransporter 9 rn
RAE230B:1393364_AT cation-chloride cotransporter 9 rn
RAT230_2:1393364_AT cation-chloride cotransporter 9 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA82423 Hypothetical protein F10E7.9 [Caenorhabditis elegans] ref|NP_495469.1| F10E7.9 [Caenorhabditis elegans] 0.0
blast CAE67542 Hypothetical protein CBG13067 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 2.6E-5
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 6.0E-8
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 2.6E-5
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 7.8E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495469.1 11 46-68,83-105,126-148,163-185,192-211,254-276,289-311,326-348,353-370,375-397,463-485
F10E7.9.2 11 46-68,83-105,126-148,163-185,192-211,254-276,289-311,326-348,353-370,375-397,463-485
F10E7.9.1 11 46-68,83-105,126-148,163-185,192-211,254-276,289-311,326-348,353-370,375-397,463-485
SNAP00000000727 11 20-42,57-79,100-122,137-159,166-185,228-250,263-285,300-322,356-373,378-400,466-488
GENEFINDER00000000737 10 46-68,83-105,126-148,163-185,192-211,254-276,289-311,342-359,364-386,452-474
Sequence
Target Sequence 
>C. ELEGANS:193378_AT
gagatctcaatcaacttgccattctttcaacaatgccttttttgataacttatgcatatg
tcaactattcatacgtatcacttgcaatgagctatgacctggctactgtacaacatgcag
atgaagttgaaactgtcggttacggatccacgaataagatgaacgatctgaatgagctgt
ttcccgagcgacgagatgtcaatattacaagcactgaggaaactgaaactggaataattt
cccaagaagcaacatggtattccatgttcagcaacagatacgtgtcttttctgggatttc
tggtgaacttggcaatccttctcttaattcatttctggtttgctgtagctcattttgtcg
ctctcatcgcgttgtattggtacattggaagagtctgcccaacttgtggtccgggaattt
caactttctcgttatgtcacatgttcaaaacagtgttttcttcattagattctttgcgat
caggtgccacaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATCTCAATCAACTTGCCATTCT 282 397 1121 Antisense
GCTATGACCTGGCTACTGTACAACA 684 303 1211 Antisense
GAAACTGTCGGTTACGGATCCACGA 513 353 1249 Antisense
CTGAATGAGCTGTTTCCCGAGCGAC 659 235 1288 Antisense
GATACGTGTCTTTTCTGGGATTTCT 705 423 1397 Antisense
AACTTGGCAATCCTTCTCTTAATTC 591 137 1426 Antisense
TGCTGTAGCTCATTTTGTCGCTCTC 463 581 1461 Antisense
GCTCTCATCGCGTTGTATTGGTACA 20 301 1480 Antisense
GGAAGAGTCTGCCCAACTTGTGGTC 444 529 1507 Antisense
TGGTCCGGGAATTTCAACTTTCTCG 272 549 1527 Antisense
ATTCTTTGCGATCAGGTGCCACAAC 517 9 1589 Antisense
Sequence Source Affymetrix Proprietary Database