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[193357_at] excitatory amino acid transporter 2 like

Description K08F4.4 /REP_DB=WormBase Gene ID /WP=CE06152 /TR=SW:Q21353 /GB=CAA93084.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=excitatory amino acid transporter 2 like
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 60      6grp_total > 14      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 261
1 2 3 4 range
A 796.5
(3)
P 		539.7
(-2.9)
P 		535.5
(-3)
P 		665.1
(-0)
P 		261
B 715.2
(3)
P 		681.7
(-0.7)
P 		661.2
(-3)
P 		714
(2.9)
P 		54
C 609.4
(-0.3)
P 		622
(1.1)
P 		584.4
(-3)
P 		639.1
(3)
P 		55
avr 707
(3) 		614.5
(-1.9) 		593.7
(-3) 		672.7
(1.2) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 332
1 2 3 4 5 6 range
A 583.9
(3)
P 		461.9
(0.5)
P 		416.6
(-0.5)
P 		294.7
(-3)
P 		344.1
(-2)
P 		294
(-3)
P 		290
B 565
(3)
P 		535.8
(2.4)
P 		442
(0.3)
P 		365.6
(-1.4)
P 		322.6
(-2.3)
P 		290.7
(-3)
P 		274
C 464.6
(2.7)
P 		476.2
(3)
P 		372.1
(0.2)
P 		431.5
(1.8)
P 		251.6
(-3)
P 		311.1
(-1.4)
P 		225
avr 537.8
(3) 		491.3
(1.8) 		410.2
(-0.2) 		363.9
(-1.4) 		306.1
(-2.8) 		298.6
(-3) 		239
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 193
1 2 3 4 range
A 290
(3)
P 		117.1
(-2.4)
P 		140
(-1.7)
P 		97.4
(-3)
P 		193
B 228.1
(3)
P 		168.4
(-1.7)
P 		153.2
(-2.9)
P 		152.2
(-3)
P 		76
C 201.7
(2.9)
P 		178.8
(1.4)
P 		203.7
(3)
P 		107.8
(-3)
P 		96
avr 239.9
(3) 		154.8
(-1.2) 		165.6
(-0.7) 		119.1
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 107.6
(-0.7)
P 		164.3
(3)
P 		90.7
(-1.8)
P 		74.7
(-2.8)
P 		103.8
(-0.9)
P 		71.6
(-3)
P 		93
B 196.6
(3)
P 		178.3
(1.9)
P 		107.3
(-2.4)
P 		96.8
(-3)
P 		131.1
(-0.9)
P 		108.4
(-2.3)
P 		100
C 172.2
(3)
P 		145.3
(1.2)
P 		94.7
(-2.1)
P 		86.8
(-2.6)
P 		81.4
(-3)
P 		104.1
(-1.5)
P 		91
avr 158.8
(2.7) 		162.6
(3) 		97.6
(-2.1) 		86.1
(-3) 		105.4
(-1.5) 		94.7
(-2.3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193357_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9604
Sequence Type Exemplar sequence
Representative Public ID K08F4.4 NCBI
Target Description K08F4.4 /REP_DB=WormBase Gene ID /WP=CE06152 /TR=SW:Q21353 /GB=CAA93084.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=excitatory amino acid transporter 2 like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069443(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069443 NCBI Caenorhabditis elegans GLutamate Transporter family member (glt-3) (glt-3) mRNA, complete cds. 11/11 None
SNAP00000005857 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10130367:10133117:1 11/11 None
GENEFINDER00000005870 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10130367:10133117:1 11/11 None
K08F4.4 ENSEMBL cdna:known chromosome:CEL140:IV:10130337:10133230:1 gene:K08F4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10130372-10133123(+) 100.0 100.0
Public Domain and Genome References
Gene Title excitatory amino acid transporter 2 like
Gene Symbol glt-3
Ensembl K08F4.4
Entrez Gene 177882 Entrez gene
SwissProt Q21353 EMBL-EBI
WormBase CE06152 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6835 dicarboxylic acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
17153 sodium:dicarboxylate symporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93084 Hypothetical protein K08F4.4 [Caenorhabditis elegans] ref|NP_501844.1| GLutamate Transporter family member (glt-3) [Caenorhabditis elegans] sp|Q21353|EAA3_CAEEL Putative sodium-dependent excitatory amino acid transporter glt-3 0.0
blast CAE59928 Hypothetical protein CBG03414 [Caenorhabditis briggsae] 0.0
blast CAE68439 Hypothetical protein CBG14224 [Caenorhabditis briggsae] 1.0E-114
Protein Domains
Database ID Description E-Value
Pfam IPR001991 EMBL-EBI Sodium:dicarboxylate symporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501844.1 7 7-29,44-66,78-100,186-205,226-248,263-285,334-356
K08F4.4 7 7-29,44-66,78-100,186-205,226-248,263-285,334-356
SNAP00000005857 7 7-29,44-66,78-100,186-205,226-248,263-285,334-356
GENEFINDER00000005870 7 7-29,44-66,78-100,186-205,226-248,263-285,334-356
Sequence
Target Sequence 
>C. ELEGANS:193357_AT
gtgactcgatttgtactgccacttggtgccacaattacaatggacggaacagcactttat
gaggctgtggctgttattttcattgctcaattacataatattaaattaagtttgatggat
ttagtgacaatcagtatcacaactacagttgcttcaattggttctggatcagttccagct
ggtttagacacaattgttattgtattgacaactgttggattaccggcaaaggatcttagt
ttgttgcttacagttgattggttacttgatcgcatccggacgtctgtcaatgttcttgga
gacagtttcggtgcaggaatcattcatcatcttacccgttcctcattactggaagctgat
accgatgagcttattcgtcagattcgtgaagacattgatattctgaataacccccatcaa
gacactcttccaatttctcatcatagtgttcaatctactattcaaaatacacagaatgca
atgcaggctccccatgtctactctaaatcagcccgagcgtctttcgcgccaattccaaac
gaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACTCGATTTGTACTGCCACTTG 386 483 1003 Antisense
ACTGCCACTTGGTGCCACAATTACA 116 99 1017 Antisense
GAGGCTGTGGCTGTTATTTTCATTG 659 405 1063 Antisense
GATTGGTTACTTGATCGCATCCGGA 154 415 1258 Antisense
GCATCCGGACGTCTGTCAATGTTCT 267 309 1274 Antisense
CAGGAATCATTCATCATCTTACCCG 352 209 1316 Antisense
CTTACCCGTTCCTCATTACTGGAAG 290 1 1333 Antisense
GATATTCTGAATAACCCCCATCAAG 146 427 1399 Antisense
TCCCCATGTCTACTCTAAATCAGCC 48 597 1491 Antisense
TCTAAATCAGCCCGAGCGTCTTTCG 301 605 1504 Antisense
GTCTTTCGCGCCAATTCCAAACGAA 475 469 1521 Antisense
Sequence Source Affymetrix Proprietary Database