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[193340_at]

Description B0025.1A /REP_DB=WormBase Gene ID /WP=CE24759 /GEN=vps-34 /TR=Q9TXI7 /GB=AAF23184.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=phosphatidylinositol 3-kinase
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 55      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 74      6grp_total > 46      4grp_total > 46      6grp_highest_sub > Cu      6grp_cgc > 35      6grp_cgc_sub > Cu      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 348
1 2 3 4 range
A 664.2
(3)
P 		409
(-1.4)
P 		316.1
(-3)
P 		440.2
(-0.9)
P 		348
B 579.3
(3)
P 		419.9
(-1.7)
P 		375.3
(-3)
P 		422.3
(-1.6)
P 		204
C 608
(3)
P 		442.8
(-0.9)
P 		407.5
(-1.8)
P 		355.8
(-3)
P 		252
avr 617.2
(3) 		423.9
(-1.6) 		366.3
(-3) 		406.1
(-2) 		251

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 499
1 2 3 4 5 6 range
A 707.5
(2.7)
P 		636.7
(1.1)
P 		719.7
(3)
P 		571.1
(-0.4)
P 		499.7
(-2)
P 		456.2
(-3)
P 		264
B 873.6
(2.2)
P 		863.3
(2.1)
P 		847
(1.8)
P 		819.1
(1.5)
P 		490.8
(-3)
P 		932
(3)
P 		441
C 590.3
(-0.2)
P 		660.9
(1)
P 		571.6
(-0.6)
P 		775.2
(3)
P 		433.5
(-3)
P 		775
(3)
P 		342
avr 723.8
(3) 		720.3
(2.9) 		712.8
(2.7) 		721.8
(3) 		474.7
(-3) 		721.1
(2.9) 		249
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 241.8
(3)
P 		88.8
(-2.2)
P 		82.6
(-2.4)
P 		64.4
(-3)
P 		177
B 184.7
(3)
P 		103.7
(-2)
P 		87
(-3)
P 		90
(-2.8)
P 		98
C 201.3
(3)
P 		127.3
(-0.1)
P 		142
(0.5)
P 		60
(-3)
P 		141
avr 209.3
(3) 		106.6
(-1.5) 		103.9
(-1.6) 		71.5
(-3) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 130.4
(-2)
P 		226.4
(3)
P 		156.7
(-0.6)
P 		144.8
(-1.2)
P 		150.8
(-0.9)
P 		111.1
(-3)
P 		115
B 304
(1.2)
P 		287.4
(0.6)
P 		205.7
(-2.7)
P 		217
(-2.3)
P 		199.4
(-3)
P 		347.5
(3)
P 		148
C 218.8
(1)
P 		201.7
(0.1)
P 		145.5
(-2.7)
P 		155.9
(-2.2)
P 		140.3
(-3)
P 		259.4
(3)
P 		119
avr 217.7
(1.3) 		238.5
(2.9) 		169.3
(-2.5) 		172.6
(-2.3) 		163.5
(-3) 		239.3
(3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193340_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1750
Sequence Type Exemplar sequence
Representative Public ID B0025.1A NCBI
Target Description B0025.1A /REP_DB=WormBase Gene ID /WP=CE24759 /GEN=vps-34 /TR=Q9TXI7 /GB=AAF23184.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=phosphatidylinositol 3-kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059340(11), NM_001025782(11), NM_001025783(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059340 NCBI Caenorhabditis elegans related to yeast Vacuolar Protein Sorting factor family member (vps-34) (vps-34) mRNA, complete cds. 11/11 None
NM_001025782 NCBI Caenorhabditis elegans related to yeast Vacuolar Protein Sorting factor family member (vps-34) (vps-34) mRNA, complete cds. 11/11 None
NM_001025783 NCBI Caenorhabditis elegans related to yeast Vacuolar Protein Sorting factor family member (vps-34) (vps-34) mRNA, complete cds. 11/11 None
SNAP00000033236 ENSEMBL cdna:SNAP chromosome:CEL140:I:6035335:6041499:1 11/11 None
B0025.1a ENSEMBL cdna:known chromosome:CEL140:I:6036621:6041592:1 gene:B0025.1 11/11 None
B0025.1c ENSEMBL cdna:known chromosome:CEL140:I:6036633:6041499:1 gene:B0025.1 11/11 None
B0025.1b ENSEMBL cdna:known chromosome:CEL140:I:6037645:6041499:1 gene:B0025.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172280 5 3 NM_001025782 193340_at (11)
NM_001025783 193340_at (11)
NM_059340 193340_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6036622-6041499(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0025.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF23184 Related to yeast vacuolar protein sorting factor protein 34, isoform a [Caenorhabditis elegans] ref|NP_491741.1| related to yeast Vacuolar Protein Sorting factor family member (vps-34) [Caenorhabditis elegans] 0.0
blast AAV34807 Related to yeast vacuolar protein sorting factor protein 34, isoform c [Caenorhabditis elegans] ref|NP_001020954.1| related to yeast Vacuolar Protein Sorting factor family member (vps-34) [Caenorhabditis elegans] 0.0
blast CAA73142 VPS34 homologue [Caenorhabditis elegans] 0.0
blast AAF23185 Related to yeast vacuolar protein sorting factor protein 34, isoform b [Caenorhabditis elegans] ref|NP_001020953.1| related to yeast Vacuolar Protein Sorting factor family member (vps-34) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 2.4E-67
Pfam IPR002420 EMBL-EBI Phosphoinositide 3-kinase, C2 domain 9.1E-52
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 3.7E-61
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 2.4E-67
Pfam IPR002420 EMBL-EBI Phosphoinositide 3-kinase, C2 domain 1.1E-56
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 3.7E-61
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 2.4E-67
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 3.7E-61
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 2.4E-67
Pfam IPR002420 EMBL-EBI Phosphoinositide 3-kinase, C2 domain 1.1E-56
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 3.7E-61
Sequence
Target Sequence 
>C. ELEGANS:193340_AT
aattatgtccgttcacttgctggttattcagttattatgtatattctgggtcttggtgat
cgtcatcttgacaatcttcttctttgtgagaatggaaaactattccacgtggattttggt
ttcatcctcggacgggatcccaaaccaatgccaccaccaatgaaattaaccagtgaaatg
gtacaagtgatgggaggtgtgaagagcaaacaatttttggaatttgtgcagcatgtcgat
tctgcctaccggattttgagacgacactcgaatgttcttttgaacttgttctcacttatg
ttagacgctggaattcctgatattgctgctgagccggataaggctattttcaagatcgaa
caacgcctccgcctggatctgtcggatgaagcagctaccaaacacattttcactc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTATGTCCGTTCACTTGCTGGTT 171 175 2227 Antisense
ATTCTGGGTCTTGGTGATCGTCATC 544 9 2269 Antisense
GTCATCTTGACAATCTTCTTCTTTG 571 467 2288 Antisense
GTGGATTTTGGTTTCATCCTCGGAC 678 489 2335 Antisense
TCCTCGGACGGGATCCCAAACCAAT 445 293 2351 Antisense
GTGCAGCATGTCGATTCTGCCTACC 275 481 2452 Antisense
GATTCTGCCTACCGGATTTTGAGAC 586 431 2464 Antisense
GAGACGACACTCGAATGTTCTTTTG 462 393 2484 Antisense
ATTCCTGATATTGCTGCTGAGCCGG 123 9 2539 Antisense
CTCCGCCTGGATCTGTCGGATGAAG 157 235 2593 Antisense
GCAGCTACCAAACACATTTTCACTC 131 313 2617 Antisense
Sequence Source Affymetrix Proprietary Database