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[193327_at] thymidine diphosphoglucose 4,6-dehydratase

Description F53B1.4 /REP_DB=WormBase Gene ID /WP=CE04644 /TR=Q20697 /GB=AAB52650.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=thymidine diphosphoglucose 4,6-dehydratase
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_high_8_8 > 55      6grp_highest_nonorm > 45      6grp_total > 82      4grp_total > 57      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6296
1 2 3 4 range
A 61.1
(-3)
A 		4824.1
(1.6)
P 		6288.5
(3)
P 		207.6
(-2.9)
P 		6227
B 75.5
(-3)
P 		4566.8
(1.3)
P 		6356.9
(3)
P 		160.6
(-2.9)
P 		6281
C 87.3
(-3)
P 		5013.2
(2)
P 		6011.4
(3)
P 		163.5
(-2.9)
P 		5924
avr 74.6
(-3) 		4801.4
(1.6) 		6218.9
(3) 		177.2
(-2.9) 		6144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3500
1 2 3 4 5 6 range
A 221.1
(-3)
P 		3718.8
(3)
P 		1822.9
(-0.3)
P 		3207.2
(2.1)
P 		3337.7
(2.3)
P 		839.9
(-1.9)
P 		3498
B 266.3
(-3)
P 		3475
(3)
P 		2410.9
(1)
P 		3448.7
(3)
P 		3100.1
(2.3)
P 		773.7
(-2.1)
P 		3209
C 218.8
(-3)
P 		3544.4
(3)
P 		2418.8
(1)
P 		3068.2
(2.1)
P 		3123.9
(2.2)
P 		876.6
(-1.8)
P 		3326
avr 235.4
(-3) 		3579.4
(3) 		2217.5
(0.6) 		3241.4
(2.4) 		3187.2
(2.3) 		830.1
(-1.9) 		3344
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2073
1 2 3 4 range
A 22.2
(-3)
A 		1047.2
(0.8)
P 		1643.9
(3)
P 		30.4
(-3)
P 		1622
B 24.1
(-3)
P 		1127.9
(1.6)
P 		1473
(3)
P 		34.2
(-3)
P 		1449
C 28.9
(-3)
P 		1441.3
(1.1)
P 		2095.2
(3)
P 		27.6
(-3)
P 		2068
avr 25.1
(-3) 		1205.5
(1.1) 		1737.4
(3) 		30.7
(-3) 		1712

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1282
1 2 3 4 5 6 range
A 40.7
(-3)
P 		1322.6
(3)
P 		397
(-1.3)
P 		813.4
(0.6)
P 		1007.2
(1.5)
P 		204.5
(-2.2)
P 		1282
B 92.7
(-3)
P 		1156.7
(2.5)
P 		585.4
(-0.5)
P 		913.4
(1.2)
P 		1259.5
(3)
P 		288.5
(-2)
P 		1167
C 81.1
(-3)
P 		1081.5
(3)
P 		615.6
(0.2)
P 		617
(0.2)
P 		1010.8
(2.6)
P 		293.4
(-1.7)
P 		1000
avr 71.5
(-3) 		1186.9
(3) 		532.7
(-0.5) 		781.3
(0.8) 		1092.5
(2.5) 		262.1
(-2) 		1115
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193327_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18105
Sequence Type Exemplar sequence
Representative Public ID F53B1.4 NCBI
Target Description F53B1.4 /REP_DB=WormBase Gene ID /WP=CE04644 /TR=Q20697 /GB=AAB52650.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=thymidine diphosphoglucose 4,6-dehydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075989(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075989 NCBI Caenorhabditis elegans F53B1.4 (F53B1.4) mRNA, complete cds. 11/11 None
SNAP00000022990 ENSEMBL cdna:SNAP chromosome:CEL140:X:2111772:2113078:-1 11/11 None
GENEFINDER00000022997 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2111772:2118149:-1 11/11 None
F53B1.4 ENSEMBL cdna:known chromosome:CEL140:X:2111772:2113078:-1 gene:F53B1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2111771-2113078(-) 100.0 100.0
Public Domain and Genome References
Gene Title thymidine diphosphoglucose 4,6-dehydratase
Ensembl F53B1.4
Entrez Gene 180525 Entrez gene
SwissProt Q20697 EMBL-EBI
WormBase CE04644 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256575_AT NAD-dependent epimerase/dehydratase family protein at
CANINE:1588566_AT similar to dTDP-D-glucose 4,6-dehydratase cfa
CANINE_2:CFA.10451.1.S1_AT similar to dTDP-D-glucose 4,6-dehydratase cfa
CANINE_2:CFAAFFX.8926.1.S1_AT similar to dTDP-D-glucose 4,6-dehydratase cfa
CHICKEN:GGAAFFX.10830.1.S1_AT similar to dTDP-D-glucose 4,6-dehydratase gga
HG-U133_PLUS_2:208249_S_AT TDP-glucose 4,6-dehydratase hs
HG-U133A:208249_S_AT TDP-glucose 4,6-dehydratase hs
HG-FOCUS:208249_S_AT TDP-glucose 4,6-dehydratase hs
HG-U133A_2:208249_S_AT TDP-glucose 4,6-dehydratase hs
HU35KSUBA:RC_AA406142_AT TDP-glucose 4,6-dehydratase hs
U133_X3P:G7657640_3P_A_AT TDP-glucose 4,6-dehydratase hs
U133_X3P:208249_3P_S_AT TDP-glucose 4,6-dehydratase hs
HG-U95AV2:41667_S_AT TDP-glucose 4,6-dehydratase hs
HG-U95AV2:41668_R_AT TDP-glucose 4,6-dehydratase hs
HG-U133_PLUS_2:237346_AT TDP-glucose 4,6-dehydratase hs
HG-U133B:237346_AT TDP-glucose 4,6-dehydratase hs
U133_X3P:HS.128804.0.A1_3P_AT TDP-glucose 4,6-dehydratase hs
HG-U95E:74358_AT TDP-glucose 4,6-dehydratase hs
MOUSE430_2:1424526_A_AT TDP-glucose 4,6-dehydratase mm
MOE430A:1424526_A_AT TDP-glucose 4,6-dehydratase mm
MOUSE430A_2:1424526_A_AT TDP-glucose 4,6-dehydratase mm
MG-U74AV2:103635_AT TDP-glucose 4,6-dehydratase mm
MU19KSUBB:TC29244_AT TDP-glucose 4,6-dehydratase (Tgds), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9225 nucleotide-sugar metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52650 Hypothetical protein F53B1.4 [Caenorhabditis elegans] ref|NP_508390.1| F53B1.4 [Caenorhabditis elegans] 0.0
blast CAE68352 Hypothetical protein CBG14085 [Caenorhabditis briggsae] 0.0
blast AAB52650 Hypothetical protein F53B1.4 [Caenorhabditis elegans] ref|NP_508390.1| F53B1.4 [Caenorhabditis elegans] 1.0E-178
blast CAE68352 Hypothetical protein CBG14085 [Caenorhabditis briggsae] 1.0E-173
blast AAB52649 Hypothetical protein F53B1.6 [Caenorhabditis elegans] ref|NP_508392.2| F53B1.6 [Caenorhabditis elegans] 9.0E-94
blast CAE60116 Hypothetical protein CBG03656 [Caenorhabditis briggsae] 9.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR001509 EMBL-EBI NAD-dependent epimerase/dehydratase 1.8E-84
Pfam IPR001509 EMBL-EBI NAD-dependent epimerase/dehydratase 2.6E-76
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 7.7E-15
Sequence
Target Sequence 
>C. ELEGANS:193327_AT
caaagtcagagtccgcatctctaccaaatccaacaaatccatatgccgctagcaaggctg
cttgtgagatggttattagatcctattggcactcctacaaacttccatacgtgatggtgc
gcatgaacaacgtctatgggccacgccaaatccataccaagctcatcccaaagttcacca
agctcgctttggatggaaaaccctacccacttatgggagacggtcttcatactagaagtt
ggatgtatgtggaagactgctcggaggctattacaagagtagcacttgaaggaacactag
gagaaatttacaatattggaactgactttgagatgacaaatattgaacttacgaagatga
ttcatttcacagtgagcaaacttttgaacagagagccaacggctccaacgtttgctccga
tcccagatagaccataccacgatcgccgttattacatcgacttctcgaaaattcgcaatg
caatgggatggcaatgcacaactccattcagcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGTCAGAGTCCGCATCTCTACC 244 193 461 Antisense
GCATCTCTACCAAATCCAACAAATC 206 309 475 Antisense
CCAACAAATCCATATGCCGCTAGCA 680 275 490 Antisense
ATTAGATCCTATTGGCACTCCTACA 493 11 535 Antisense
ACTCCTACAAACTTCCATACGTGAT 599 97 551 Antisense
AGTTCACCAAGCTCGCTTTGGATGG 677 61 632 Antisense
TGGATGGAAAACCCTACCCACTTAT 309 549 650 Antisense
GAGAGCCAACGGCTCCAACGTTTGC 639 339 851 Antisense
TCCGATCCCAGATAGACCATACCAC 375 593 876 Antisense
GATCGCCGTTATTACATCGACTTCT 428 417 901 Antisense
GCAATGCACAACTCCATTCAGCGAA 567 321 951 Antisense
Sequence Source Affymetrix Proprietary Database