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[193326_s_at]

Description T05H10.6 /REP_DB=WormBase Gene ID /WP=CE01643 /TR=SW:P52899 /GB=CAA87793.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=pyruvate dehydrogenase E1 alpha subunit
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 40      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 26      6grp_total > 41      4grp_total > 60      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1468
1 2 3 4 range
A 5111.7
(2.3)
P 		5132.3
(2.4)
P 		5283.5
(3)
P 		3840.5
(-3)
P 		1443
B 5149.7
(2.9)
P 		4572.6
(-1.1)
P 		5164.9
(3)
P 		4292.8
(-3)
P 		872
C 4601.4
(-0)
P 		4427.8
(-0.8)
P 		5308.5
(3)
P 		3899.4
(-3)
P 		1409
avr 4954.3
(1.6) 		4710.9
(0.4) 		5252.3
(3) 		4010.9
(-3) 		1241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3811
1 2 3 4 5 6 range
A 5917.2
(0.9)
P 		5464.4
(-0.3)
P 		6248.8
(1.8)
P 		6714.6
(3)
P 		4417.8
(-3)
P 		5085.1
(-1.3)
P 		2297
B 5690.6
(1.2)
P 		5938.1
(1.7)
P 		6490.8
(3)
P 		5289
(0.3)
P 		4743.6
(-1)
P 		3849.2
(-3)
P 		2642
C 5374.6
(-0.4)
P 		5571.7
(-0.1)
P 		7547.3
(3)
P 		5004.2
(-1)
P 		5051.7
(-0.9)
P 		3736.2
(-3)
P 		3811
avr 5660.8
(0.4) 		5658.1
(0.4) 		6762.3
(3) 		5669.3
(0.4) 		4737.7
(-1.8) 		4223.5
(-3) 		2539
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1299
1 2 3 4 range
A 1861.2
(3)
P 		1114.1
(-0.5)
P 		1381.2
(0.8)
P 		562.2
(-3)
P 		1299
B 1642.2
(3)
P 		1129.3
(-1.2)
P 		1196.8
(-0.7)
P 		914.8
(-3)
P 		727
C 1523.2
(1.4)
P 		1273
(0.1)
P 		1850.2
(3)
P 		657.5
(-3)
P 		1193
avr 1675.5
(3) 		1172.1
(-0.1) 		1476.1
(1.8) 		711.5
(-3) 		964

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 986
1 2 3 4 5 6 range
A 1090.8
(-3)
P 		1943.4
(3)
P 		1360.9
(-1.1)
P 		1703
(1.3)
P 		1333.1
(-1.3)
P 		1238.1
(-2)
P 		853
B 1980
(3)
P 		1976.6
(3)
P 		1576.2
(-1.2)
P 		1400.9
(-3)
P 		1927.3
(2.5)
P 		1435.4
(-2.6)
P 		579
C 1992.1
(3)
P 		1700.1
(1.2)
P 		1920.9
(2.6)
P 		1006.3
(-3)
P 		1634.7
(0.8)
P 		1250.7
(-1.5)
P 		986
avr 1687.6
(1) 		1873.4
(3) 		1619.3
(0.3) 		1370.1
(-2.3) 		1631.7
(0.4) 		1308.1
(-3) 		565
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193326_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3675
Sequence Type Exemplar sequence
Representative Public ID T05H10.6 NCBI
Target Description T05H10.6 /REP_DB=WormBase Gene ID /WP=CE01643 /TR=SW:P52899 /GB=CAA87793.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=pyruvate dehydrogenase E1 alpha subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182153(11), NM_063292(11), NM_001027149(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182153 NCBI Caenorhabditis elegans T05H10.6b (T05H10.6) mRNA, complete cds. 11/11 None
NM_063292 NCBI Caenorhabditis elegans T05H10.6a (T05H10.6) mRNA, complete cds. 11/11 None
NM_001027149 NCBI Caenorhabditis elegans Ubiquitin Fusion Degradation (yeast UFD homolog) family member (ufd-2) (ufd-2) mRNA, complete cds. 11/11 None
SNAP00000019967 ENSEMBL cdna:SNAP chromosome:CEL140:II:8066403:8067726:1 11/11 None
GENEFINDER00000019971 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8066403:8067726:1 11/11 None
T05H10.5c ENSEMBL cdna:known chromosome:CEL140:II:8062664:8068029:1 gene:T05H10.5 11/11 A 
T05H10.6b.2 ENSEMBL cdna:known chromosome:CEL140:II:8066208:8067996:1 gene:T05H10.6 11/11 None
T05H10.6b.1 ENSEMBL cdna:known chromosome:CEL140:II:8066208:8068105:1 gene:T05H10.6 11/11 None
T05H10.6a.3 ENSEMBL cdna:known chromosome:CEL140:II:8066365:8067726:1 gene:T05H10.6 11/11 None
T05H10.6a.1 ENSEMBL cdna:known chromosome:CEL140:II:8066366:8067726:1 gene:T05H10.6 11/11 None
T05H10.6a.2 ENSEMBL cdna:known chromosome:CEL140:II:8066369:8067726:1 gene:T05H10.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174295 3 1 NM_001027149 193326_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8066399-8067723(+) 100.0 100.0
Public Domain and Genome References
Ensembl T05H10.5
T05H10.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD59156 Hypothetical protein T05H10.6b [Caenorhabditis elegans] ref|NP_871953.1| T05H10.6b [Caenorhabditis elegans] 0.0
blast CAA87793 Hypothetical protein T05H10.6a [Caenorhabditis elegans] ref|NP_495693.1| T05H10.6a [Caenorhabditis elegans] sp|P52899|ODPA_CAEEL Probable pyruvate dehydrogenase E1 component alpha subunit, mitochondrial precursor (PDHE1-A) 0.0
blast CAE67764 Hypothetical protein CBG13339 [Caenorhabditis briggsae] 0.0
blast CAH04720 Hypothetical protein T05H10.5c [Caenorhabditis elegans] ref|NP_001022320.1| Ubiquitin Fusion Degradation (yeast UFD homolog) family member (ufd-2) [Caenorhabditis elegans] 0.0
blast CAA87792 Hypothetical protein T05H10.5a [Caenorhabditis elegans] ref|NP_495692.1| Ubiquitin Fusion Degradation (yeast UFD homolog) family member (ufd-2) [Caenorhabditis elegans] sp|Q09349|UBE4_CAEEL Probable ubiquitin conjugation factor E4 0.0
blast CAC70105 Hypothetical protein T05H10.5b [Caenorhabditis elegans] ref|NP_495691.1| Ubiquitin Fusion Degradation (yeast UFD homolog) family member (ufd-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001017 EMBL-EBI Dehydrogenase, E1 component 1.0E-126
Pfam IPR001017 EMBL-EBI Dehydrogenase, E1 component 1.0E-126
Pfam IPR003613 EMBL-EBI Zn-finger, modified RING 2.1E-56
Sequence
Target Sequence 
>C. ELEGANS:193326_S_AT
caaggacaacttttcgaggcgactaacatggctaaactttgggatcttcctgttctgttc
gtttgcgaaaacaatggattcggaatgggaactactgctgaacgttcatctgcttctacc
gagtattatactagaggagactatgttcctggaatctgggttgatggtatggacattctc
gctgttcgcgaggctaccaagtgggctaaggaatattgtgacagtggtaaaggaccactt
atgatggaaatggccacctatagataccatggacactctatgtctgacccaggaaccagc
taccgtacccgtgaagagattcaagaagttcgtaaaacaagagacccaattactggattc
aaggaccgtatcattacatcttcattggccacagaggaagaacttaaggctattgacaag
gaagttcgcaaagaggtcgatgaagcgttgaagattgctacatcagacggtgttctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGGACAACTTTTCGAGGCGACTA 705 183 595 Antisense
AACTTTGGGATCTTCCTGTTCTGTT 500 139 629 Antisense
AACTACTGCTGAACGTTCATCTGCT 684 139 684 Antisense
TTCATCTGCTTCTACCGAGTATTAT 309 689 699 Antisense
GGACATTCTCGCTGTTCGCGAGGCT 219 525 765 Antisense
TTCGCGAGGCTACCAAGTGGGCTAA 389 681 779 Antisense
ACCATGGACACTCTATGTCTGACCC 154 87 860 Antisense
TATGTCTGACCCAGGAACCAGCTAC 110 657 873 Antisense
CAGCTACCGTACCCGTGAAGAGATT 526 201 891 Antisense
ATCATTACATCTTCATTGGCCACAG 644 29 964 Antisense
GATTGCTACATCAGACGGTGTTCTC 182 435 1047 Antisense
Sequence Source Affymetrix Proprietary Database