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[193320_s_at] calcium/calmodulin dependent protein kinase

Description ZC373.4 /REP_DB=WormBase Gene ID /WP=CE02377 /TR=Q23260 /GB=CAA88976.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=calcium-calmodulin dependent protein kinase
Cluster 6grp_high1_10_10 > 68      6grp_total > 79      4grp_total > 12      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 153.3
(1.7)
P 		146.7
(1.4)
A 		37.2
(-3)
A 		186.3
(3)
P 		149
B 117.9
(-1.5)
P 		75.1
(-2.4)
A 		50.1
(-3)
A 		312.7
(3)
P 		263
C 153.3
(-0.4)
P 		73.2
(-2.4)
A 		46.8
(-3)
A 		294.3
(3)
P 		248
avr 141.5
(-0.4) 		98.3
(-1.5) 		44.7
(-3) 		264.4
(3) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 77.5
(-2.9)
P 		156.7
(1.3)
P 		75
(-3)
P 		101.4
(-1.6)
P 		99.2
(-1.7)
P 		190
(3)
P 		115
B 111.1
(2.2)
P 		78.8
(-0.8)
A 		72.1
(-1.4)
P 		55.4
(-3)
A 		78.4
(-0.9)
P 		119.9
(3)
P 		65
C 111.7
(-0.6)
P 		114.2
(-0.5)
P 		115.3
(-0.4)
P 		67.4
(-3)
P 		115.1
(-0.4)
P 		177.6
(3)
P 		110
avr 100.1
(-1.3) 		116.6
(-0.1) 		87.5
(-2.1) 		74.7
(-3) 		97.6
(-1.4) 		162.5
(3) 		88
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 55.8
(3)
P 		31.8
(-0.1)
A 		9.7
(-3)
A 		27.3
(-0.7)
P 		46
B 37.6
(-0.2)
P 		18.6
(-2.2)
A 		11.6
(-3)
A 		66.6
(3)
P 		55
C 50.7
(3)
P 		21.1
(-2.2)
A 		16.3
(-3)
A 		49.6
(2.8)
P 		34
avr 48
(3) 		23.8
(-1.1) 		12.5
(-3) 		47.8
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 14.3
(-3)
P 		55.7
(3)
P 		16.3
(-2.7)
P 		25.7
(-1.3)
P 		29.9
(-0.7)
P 		46.3
(1.6)
P 		41
B 38.6
(1.8)
P 		26.2
(-0.7)
A 		17.5
(-2.4)
P 		14.7
(-3)
A 		31.9
(0.4)
P 		44.7
(3)
P 		30
C 41.4
(0.6)
P 		34.8
(-0.2)
P 		29.4
(-0.9)
P 		13.6
(-3)
P 		37.2
(0.1)
P 		59.5
(3)
P 		46
avr 31.4
(-0.5) 		38.9
(0.9) 		21.1
(-2.4) 		18
(-3) 		33
(-0.2) 		50.2
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193320_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17119
Sequence Type Exemplar sequence
Representative Public ID ZC373.4 NCBI
Target Description ZC373.4 /REP_DB=WormBase Gene ID /WP=CE02377 /TR=Q23260 /GB=CAA88976.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=calcium-calmodulin dependent protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077288(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077288 NCBI Caenorhabditis elegans ZC373.4 (ZC373.4) mRNA, complete cds. 11/11 None
SNAP00000017664 ENSEMBL cdna:SNAP chromosome:CEL140:X:10062716:10067814:-1 11/11 None
GENEFINDER00000017671 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10062716:10069746:-1 11/11 None
ZC373.4 ENSEMBL cdna:known chromosome:CEL140:X:10062380:10069713:-1 gene:ZC373.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10062715-10069713(-) 100.0 100.0
Public Domain and Genome References
Gene Title calcium/calmodulin dependent protein kinase
Ensembl ZC373.4
Entrez Gene 181217 Entrez gene
SwissProt Q23260 EMBL-EBI
WormBase CE02377 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88976 Hypothetical protein ZC373.4 [Caenorhabditis elegans] ref|NP_509689.1| ZC373.4 [Caenorhabditis elegans] 0.0
blast CAE58772 Hypothetical protein CBG01967 [Caenorhabditis briggsae] 0.0
blast AAB25794 155 kda myosin light chain kinase homolog [Bos taurus] sp|Q28824|MYLK_BOVIN Myosin light chain kinase, smooth muscle (MLCK) [Contains: Telokin] 4.0E-71
Protein Families
Method ID Description E-Value
hanks 2.1.15 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; MLCK-M 2.0E-79
hanks 2.1.15 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; MLCK-M 4.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.1E-78
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-33
Pfam IPR000719 EMBL-EBI Protein kinase 2.1E-78
Sequence
Target Sequence 
>C. ELEGANS:193320_S_AT
aacacctcgaaatttctacggatgggaaactaggtgttctcaaaacaaaagacacaactt
cgatgaaattatctccggactcaagcagcgatacatcgtcaagcaaaaatgtgcggatcc
ttttaacagatgaacagtcagttaagtcgagtgtaagatctcatcctagtgttagcacca
gagatagttcagaggaaaggaagaaggttcgctttgccggtgatgttgggacaccgacga
aactaaatccccctccactttttgggaatggtcttcagaaaatgagatctgaaagtagtt
tacacgaaaaaatcaacggactcactaccaaaaagagtcatgatgacgtgtcgctcgcta
gaaaaacatcactgttcacagatattgaataccaaccacgcgaagatttttcgtttgaag
cattgaaagaaaaattgacacgacgggtcagctatggatatgacgatgatggcaaacgtg
gaaaacaaatggtactaattccacctacaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACACCTCGAAATTTCTACGGATGG 247 137 3089 Antisense
GAAATTATCTCCGGACTCAAGCAGC 77 363 3153 Antisense
ATGTGCGGATCCTTTTAACAGATGA 521 49 3197 Antisense
AGATCTCATCCTAGTGTTAGCACCA 650 65 3244 Antisense
AGGAAGAAGGTTCGCTTTGCCGGTG 505 1 3286 Antisense
GTGATGTTGGGACACCGACGAAACT 289 481 3308 Antisense
AGTCATGATGACGTGTCGCTCGCTA 592 61 3424 Antisense
GTCGCTCGCTAGAAAAACATCACTG 472 475 3438 Antisense
GAATACCAACCACGCGAAGATTTTT 372 325 3475 Antisense
ACACGACGGGTCAGCTATGGATATG 620 109 3526 Antisense
AATGGTACTAATTCCACCTACAAAT 117 163 3576 Antisense
Sequence Source Affymetrix Proprietary Database