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[193316_s_at] Domain in SPla and the ryanodine Receptor.

Description Y54E5A.7 /REP_DB=WormBase Gene ID /WP=CE20318 /TR=Q9XWK4 /GB=CAA21656.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Domain in SPla and the ryanodine Receptor.
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 28      4grp_high_8_8 > 40      6grp_highest_nonorm > 81      6grp_total > 27      4grp_total > 39      6grp_highest_sub > Cu      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 276.3
(2.9)
P 		225.6
(0.6)
P 		146.8
(-3)
P 		278.9
(3)
A 		132
B 240.7
(3)
P 		201.5
(-0.6)
P 		224.9
(1.5)
P 		175.7
(-3)
P 		65
C 286.4
(3)
P 		166.7
(-3)
P 		200.3
(-1.3)
P 		180.3
(-2.3)
P 		120
avr 267.8
(3) 		197.9
(-2.4) 		190.7
(-3) 		211.6
(-1.4) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 191
1 2 3 4 5 6 range
A 367.1
(3)
P 		288.8
(0.4)
P 		281.5
(0.2)
P 		184.5
(-3)
P 		257.7
(-0.6)
P 		347.7
(2.4)
P 		183
B 338.7
(2.9)
P 		341.3
(3)
P 		176.1
(-3)
P 		273.1
(0.5)
P 		239.1
(-0.7)
P 		288.6
(1.1)
P 		165
C 333.4
(3)
P 		256.1
(-0.8)
P 		271.3
(-0.1)
P 		259.9
(-0.6)
P 		283.9
(0.6)
P 		212.1
(-3)
P 		121
avr 346.4
(3) 		295.4
(0.1) 		243
(-2.8) 		239.2
(-3) 		260.2
(-1.8) 		282.8
(-0.6) 		107
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 100.6
(3)
P 		49
(-2)
P 		38.4
(-3)
P 		40.8
(-2.8)
A 		62
B 76.8
(3)
P 		49.8
(-1.1)
P 		52.1
(-0.8)
P 		37.4
(-3)
P 		39
C 94.8
(3)
P 		47.9
(-1.4)
P 		69.8
(0.7)
P 		30.4
(-3)
P 		64
avr 90.7
(3) 		48.9
(-1.6) 		53.4
(-1.1) 		36.2
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 67.7
(-0.8)
P 		102.7
(3)
P 		61.3
(-1.4)
P 		46.8
(-3)
P 		77.8
(0.3)
P 		84.7
(1.1)
P 		56
B 117.9
(3)
P 		113.6
(2.7)
P 		42.8
(-3)
P 		72.3
(-0.6)
P 		97.1
(1.3)
P 		107.6
(2.2)
P 		75
C 123.6
(3)
P 		78.1
(-0.8)
P 		69
(-1.6)
P 		52.3
(-3)
P 		91.9
(0.3)
P 		71
(-1.4)
P 		71
avr 103.1
(3) 		98.1
(2.3) 		57.7
(-2.9) 		57.1
(-3) 		88.9
(1.1) 		87.8
(1) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193316_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1445
Sequence Type Exemplar sequence
Representative Public ID Y54E5A.7 NCBI
Target Description Y54E5A.7 /REP_DB=WormBase Gene ID /WP=CE20318 /TR=Q9XWK4 /GB=CAA21656.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Domain in SPla and the ryanodine Receptor.
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061154(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061154 NCBI Caenorhabditis elegans Y54E5A.7 (Y54E5A.7) mRNA, complete cds. 11/11 None
SNAP00000041547 ENSEMBL cdna:SNAP chromosome:CEL140:I:14705390:14722870:1 11/11 None
GENEFINDER00000041554 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14719303:14722870:1 11/11 None
Y54E5A.7 ENSEMBL cdna:known chromosome:CEL140:I:14719199:14723148:1 gene:Y54E5A.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14719233-14722801(+) 100.0 100.0
Public Domain and Genome References
Gene Title Domain in SPla and the ryanodine Receptor.
Ensembl Y54E5A.7
Entrez Gene 173332 Entrez gene
SwissProt Q9XWK4 EMBL-EBI
WormBase CE20318 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21656 Hypothetical protein Y54E5A.7 [Caenorhabditis elegans] ref|NP_493555.1| Y54E5A.7 [Caenorhabditis elegans] 0.0
blast CAA21661 Hypothetical protein Y54E5A.3 [Caenorhabditis elegans] ref|NP_493551.1| Y54E5A.3 [Caenorhabditis elegans] 1.0E-146
blast CAE72564 Hypothetical protein CBG19748 [Caenorhabditis briggsae] 1.0E-137
blast XP_692736 PREDICTED: similar to Ran-binding protein 10 isoform 1 [Danio rerio] 5.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 4.2E-38
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 4.2E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000041547 1 278-300
Sequence
Target Sequence 
>C. ELEGANS:193316_S_AT
gatgcgcgggaatattgctccagaagcacgaaaagttgttgagatagcaacatcaatggt
ctcaagtccagaacgccctgcaaaacaccctctgagcttcgccctccggagatttattgc
aaattccgtagtcgatatgatttgttattatgtccatcataaacgtgaaggaaaatcaac
aaaacggccccgtggaacatctactggggaaataccaggctcatcaagtttacgaatgtt
ctctgaattacgtaacctattcaccggttggaagggtcttcatatcgattattcgactaa
tgacaagaaaatggctggaattcatttcgtccggaaaatgattctcgaggagccaaactt
caatgaaaaagagataatctatacggatgagcccatggaattggctcacgagcggccaga
tcctccacctcgagagattat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCGCGGGAATATTGCTCCAGAA 168 415 1410 Antisense
TCAATGGTCTCAAGTCCAGAACGCC 156 633 1462 Antisense
TTCGCCCTCCGGAGATTTATTGCAA 211 697 1507 Antisense
CGGCCCCGTGGAACATCTACTGGGG 378 241 1594 Antisense
AATACCAGGCTCATCAAGTTTACGA 613 169 1620 Antisense
GAATGTTCTCTGAATTACGTAACCT 16 331 1643 Antisense
TATTCACCGGTTGGAAGGGTCTTCA 626 661 1667 Antisense
AAGGGTCTTCATATCGATTATTCGA 27 161 1681 Antisense
GGCTGGAATTCATTTCGTCCGGAAA 353 539 1722 Antisense
TAATCTATACGGATGAGCCCATGGA 485 635 1784 Antisense
CAGATCCTCCACCTCGAGAGATTAT 334 205 1826 Antisense
Sequence Source Affymetrix Proprietary Database