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[193299_at] 5-formyltetrahydrofolate cyclo-ligase

Description Y106G6E.4 /REP_DB=WormBase Gene ID /WP=CE20408 /TR=SW:Q9XWE6 /GB=CAA21728.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=5-formyltetrahydrofolate cyclo-ligase
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 30      4grp_highest_8_8 > 23      4grp_high_8_8 > 05      6grp_highest_nonorm > 71      6grp_total > 42      4grp_total > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 903
1 2 3 4 range
A 393.2
(-1.8)
P 		332.5
(-2.2)
P 		217
(-3)
P 		1119.6
(3)
P 		903
B 348.6
(-2.3)
P 		259.2
(-3)
P 		309.4
(-2.6)
P 		1036.1
(3)
P 		777
C 419.4
(-1.8)
P 		280.5
(-3)
P 		281.9
(-3)
P 		995.7
(3)
P 		715
avr 387.1
(-2.1) 		290.7
(-2.8) 		269.4
(-3) 		1050.5
(3) 		781

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 301
1 2 3 4 5 6 range
A 320.1
(-1)
P 		334.9
(-0.7)
P 		519.4
(3)
P 		360.4
(-0.2)
P 		258.4
(-2.2)
P 		218.2
(-3)
P 		301
B 395.7
(-0.8)
P 		407.9
(-0.4)
P 		505.8
(3)
P 		340.5
(-2.7)
P 		336.8
(-2.8)
P 		332.3
(-3)
P 		174
C 341.3
(1.4)
P 		367.6
(3)
P 		309.1
(-0.5)
P 		363.4
(2.7)
P 		268.4
(-3)
P 		307.4
(-0.6)
P 		99
avr 352.4
(-0.5) 		370.1
(0.2) 		444.8
(3) 		354.8
(-0.4) 		287.9
(-2.9) 		286
(-3) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 143.2
(1.8)
P 		72.2
(-2.1)
P 		56.7
(-3)
P 		163.9
(3)
P 		107
B 111.2
(-1.2)
P 		64
(-3)
P 		71.7
(-2.7)
P 		220.8
(3)
P 		157
C 138.8
(1)
P 		80.6
(-3)
P 		98.3
(-1.8)
P 		167.9
(3)
P 		87
avr 131.1
(0.2) 		72.3
(-3) 		75.6
(-2.8) 		184.2
(3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 59
(-2.5)
P 		119.1
(3)
P 		113.1
(2.5)
P 		91.4
(0.5)
P 		78
(-0.7)
P 		53.1
(-3)
P 		66
B 137.7
(3)
P 		135.8
(2.8)
P 		122.8
(1.1)
P 		90.2
(-3)
P 		136.8
(2.9)
P 		123.9
(1.3)
P 		48
C 126.5
(3)
P 		112.2
(1.4)
P 		78.7
(-2.4)
P 		73.1
(-3)
P 		86.9
(-1.4)
P 		102.9
(0.3)
P 		53
avr 107.7
(0.6) 		122.4
(3) 		104.9
(0.2) 		84.9
(-3) 		100.6
(-0.5) 		93.3
(-1.7) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193299_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18825
Sequence Type Exemplar sequence
Representative Public ID Y106G6E.4 NCBI
Target Description Y106G6E.4 /REP_DB=WormBase Gene ID /WP=CE20408 /TR=SW:Q9XWE6 /GB=CAA21728.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=5-formyltetrahydrofolate cyclo-ligase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060291(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060291 NCBI Caenorhabditis elegans Y106G6E.4 (Y106G6E.4) mRNA, complete cds. 11/11 None
SNAP00000023677 ENSEMBL cdna:SNAP chromosome:CEL140:I:10229911:10230857:-1 11/11 None
GENEFINDER00000023701 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10229911:10230899:-1 11/11 None
Y106G6E.4 ENSEMBL cdna:known chromosome:CEL140:I:10229756:10230899:-1 gene:Y106G6E.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10229841-10230830(-) 100.0 100.0
Public Domain and Genome References
Gene Title 5-formyltetrahydrofolate cyclo-ligase
Ensembl Y106G6E.4
Entrez Gene 190922 Entrez gene
SwissProt Q9XWE6 EMBL-EBI
WormBase CE20408 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245983_AT 5-formyltetrahydrofolate cyclo-ligase (5-FCL) at
CANINE_2:CFAAFFX.21583.1.S1_AT similar to 5-formyltetrahydrofolate cyclo-ligase (5,10-methenyl-tetrahydrofolate synthetase) (Methenyl-THF synthetase) (MTHFS) cfa
DROSOPHILA_2:1640379_S_AT dm
DROSGENOME1:147768_AT dm
CHICKEN:GGA.7827.1.S1_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) gga
HG-U133_PLUS_2:210242_X_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U133A:210242_X_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U133A_2:210242_X_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U133_PLUS_2:203433_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U133A_2:203433_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U133A:203433_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-FOCUS:203433_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U95AV2:33737_F_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HU35KSUBA:L38928_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HUGENEFL:L38928_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HU35KSUBC:RC_W20487_S_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
U133_X3P:G5453745_3P_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
U133_X3P:G10442005_3P_S_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U95AV2:33738_R_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U95AV2:39064_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
HG-U95D:86453_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) hs
MOUSE430_2:1443021_AT 5, 10-methenyltetrahydrofolate synthetase mm
MOE430B:1443021_AT 5, 10-methenyltetrahydrofolate synthetase mm
MU11KSUBB:X52634_F_AT 5, 10-methenyltetrahydrofolate synthetase mm
MG-U74BV2:114690_AT 5, 10-methenyltetrahydrofolate synthetase mm
MOE430A:1460257_A_AT 5, 10-methenyltetrahydrofolate synthetase mm
MOUSE430A_2:1460257_A_AT 5, 10-methenyltetrahydrofolate synthetase mm
MOUSE430_2:1460257_A_AT 5, 10-methenyltetrahydrofolate synthetase mm
MU19KSUBB:TC27080_S_AT 5, 10-methenyltetrahydrofolate synthetase, mRNA (cDNA clone MGC:37660 IMAGE:5026828) mm
RG-U34C:RC_AI178809_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
RAE230B:1381768_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
RAT230_2:1381768_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
RAE230B:1392719_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
RAT230_2:1392719_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
RAE230B:1384370_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
RAT230_2:1384370_AT 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) (predicted) rn
YEAST_2:1770451_AT 5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21728 Hypothetical protein Y106G6E.4 [Caenorhabditis elegans] ref|NP_492692.1| Y106G6E.4 [Caenorhabditis elegans] sp|Q9XWE6|MTHFS_CAEEL Putative 5-formyltetrahydrofolate cyclo-ligase (5,10-methenyl-tetrahydrofolate synthetase) (Methenyl-THF synthetase) (MTHFS) 1.0E-116
blast CAA21728 Hypothetical protein Y106G6E.4 [Caenorhabditis elegans] ref|NP_492692.1| Y106G6E.4 [Caenorhabditis elegans] sp|Q9XWE6|MTHFS_CAEEL Putative 5-formyltetrahydrofolate cyclo-ligase (5,10-methenyl-tetrahydrofolate synthetase) (Methenyl-THF synthetase) (MTHFS) 1.0E-108
blast CAE66846 Hypothetical protein CBG12218 [Caenorhabditis briggsae] 2.0E-91
blast CAE66846 Hypothetical protein CBG12218 [Caenorhabditis briggsae] 8.0E-86
blast NP_006432 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) [Homo sapiens] gb|AAH19921.1| 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) [Homo sapiens] sp|P49914|MTHFS_HUMAN 5-formyltetrahydrofolate cyclo-ligase (5,10-methenyl-tetrahydrofolate synthetase) (Methenyl-THF synthetase) (MTHFS) gb|AAC41945.1| 5,10-methenyltetrahydrofolate synthetase 3.0E-32
Protein Domains
Database ID Description E-Value
Pfam IPR002698 EMBL-EBI 5-formyltetrahydrofolate cyclo-ligase 7.3E-82
Pfam IPR002698 EMBL-EBI 5-formyltetrahydrofolate cyclo-ligase 2.0E-75
Sequence
Target Sequence 
>C. ELEGANS:193299_AT
gaagttttcattccacagttcacgaagggctcaactgcaatggatatggttcgagttccg
gatcaaactgcttttgacaatcttccttcaacactttggggaatcagacagccagaaccg
aaatggaaatggcaaagctatcatgaaacaggaccactggatttgatccttgcacccggc
gttgccttctcaccatatggacttcgttgtggtcatggaaaaggatattacgatcgcttc
ttctccactcatcacaaacattttccagaaaactccccaaaaaagataggattggctctt
cgtgagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTTTTCATTCCACAGTTCACGA 429 337 235 Antisense
GTTCACGAAGGGCTCAACTGCAATG 333 441 252 Antisense
GGATATGGTTCGAGTTCCGGATCAA 480 509 276 Antisense
AAACTGCTTTTGACAATCTTCCTTC 600 127 299 Antisense
ACAATCTTCCTTCAACACTTTGGGG 646 111 311 Antisense
GGGAATCAGACAGCCAGAACCGAAA 79 495 333 Antisense
GGACCACTGGATTTGATCCTTGCAC 387 523 385 Antisense
CCTTCTCACCATATGGACTTCGTTG 332 267 419 Antisense
AAAGGATATTACGATCGCTTCTTCT 343 121 454 Antisense
TTCTCCACTCATCACAAACATTTTC 599 693 475 Antisense
AAGATAGGATTGGCTCTTCGTGAGC 439 155 517 Antisense
Sequence Source Affymetrix Proprietary Database