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[193286_s_at] 60s ribosomal protein L35A

Description F10E7.7 /REP_DB=WormBase Gene ID /WP=CE04362 /GEN=rpl-33 /TR=SW:P49180 /GB=AAA82422.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=60s ribosomal protein L35A
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 30      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 09      6grp_total > 42      4grp_total > 17      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22866
1 2 3 4 range
A 29572.7
(-3)
P 		37934.8
(-0.7)
P 		37645.8
(-0.8)
P 		51147.7
(3)
P 		21575
B 31231.8
(-3)
P 		34154.9
(-1.2)
P 		37254
(0.6)
P 		41165.3
(3)
P 		9934
C 28597.1
(-2.9)
P 		30995.3
(-2.1)
P 		28282.2
(-3)
P 		46906.6
(3)
P 		18624
avr 29800.5
(-3) 		34361.7
(-1.4) 		34394
(-1.3) 		46406.5
(3) 		16606

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11396
1 2 3 4 5 6 range
A 31872.3
(2.9)
P 		27189.4
(-1)
P 		31980.3
(3)
P 		27353.8
(-0.8)
P 		24747.1
(-3)
P 		27617.1
(-0.6)
P 		7233
B 26490.5
(2.2)
P 		25636.8
(1.4)
P 		27417.6
(3)
P 		26132.7
(1.9)
P 		21739.6
(-2)
P 		20584.1
(-3)
P 		6834
C 23395.8
(-1.5)
P 		26540.4
(0.8)
P 		29542.4
(3)
P 		26652.1
(0.9)
P 		24670.2
(-0.6)
P 		21341.2
(-3)
P 		8201
avr 27252.9
(0.8) 		26455.5
(0) 		29646.8
(3) 		26712.9
(0.3) 		23719
(-2.5) 		23180.8
(-3) 		6466
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3280
1 2 3 4 range
A 10767.4
(3)
P 		8234.6
(-1.6)
P 		9841.3
(1.3)
P 		7487
(-3)
P 		3280
B 9959.6
(3)
P 		8435.5
(-3)
P 		8632.6
(-2.2)
P 		8772.3
(-1.7)
P 		1524
C 9466.4
(1.8)
P 		8911.3
(0.1)
P 		9857.2
(3)
P 		7909.2
(-3)
P 		1948
avr 10064.5
(3) 		8527.1
(-1.6) 		9443.7
(1.1) 		8056.2
(-3) 		2008

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4310
1 2 3 4 5 6 range
A 5875.4
(-3)
P 		9669.9
(3)
P 		6964.6
(-1.3)
P 		6937.6
(-1.3)
P 		7467.6
(-0.5)
P 		6723.9
(-1.7)
P 		3795
B 9217.4
(3)
P 		8533.4
(1.4)
P 		6657.8
(-3)
P 		6921.7
(-2.4)
P 		8832.5
(2.1)
P 		7675.7
(-0.6)
P 		2560
C 8671.8
(3)
P 		8098.3
(2)
P 		7519
(0.9)
P 		5359.8
(-3)
P 		7982.9
(1.8)
P 		7144.1
(0.2)
P 		3312
avr 7921.5
(0.9) 		8767.2
(3) 		7047.1
(-1.4) 		6406.4
(-3) 		8094.3
(1.3) 		7181.2
(-1) 		2361
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193286_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4248
Sequence Type Exemplar sequence
Representative Public ID F10E7.7 NCBI
Target Description F10E7.7 /REP_DB=WormBase Gene ID /WP=CE04362 /GEN=rpl-33 /TR=SW:P49180 /GB=AAA82422.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=60s ribosomal protein L35A
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063067(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063067 NCBI Caenorhabditis elegans Ribosomal Protein, Large subunit family member (rpl-33) (rpl-33) mRNA, complete cds. 11/11 None
SNAP00000000726 ENSEMBL cdna:SNAP chromosome:CEL140:II:7105499:7108693:1 11/11 None
GENEFINDER00000000740 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7105499:7106187:1 11/11 None
F10E7.7.1 ENSEMBL cdna:known chromosome:CEL140:II:7105486:7106392:1 gene:F10E7.7 11/11 None
F10E7.7.2 ENSEMBL cdna:known chromosome:CEL140:II:7105493:7106247:1 gene:F10E7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7105494-7106183(+) 100.0 100.0
Public Domain and Genome References
Gene Title 60s ribosomal protein L35A
Gene Symbol rpl-33
Ensembl F10E7.7
Entrez Gene 174166 Entrez gene
SwissProt P49180 EMBL-EBI
WormBase CE04362 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256065_AT 60S ribosomal protein L35a (RPL35aA) at
CANINE:1583720_S_AT similar to ribosomal protein L35a cfa
CANINE:1583719_AT similar to ribosomal protein L35a cfa
CANINE_2:CFA.704.1.S1_AT similar to ribosomal protein L35a cfa
CANINE_2:CFAAFFX.19688.1.S1_S_AT similar to ribosomal protein L35a cfa
DROSOPHILA_2:1623995_AT Ribosomal protein L35A dm
DROSGENOME1:149334_AT Ribosomal protein L35A dm
CHICKEN:GGA.4459.4.S1_A_AT ribosomal protein L35a gga
CHICKEN:GGAAFFX.20205.1.S1_S_AT ribosomal protein L35a gga
CHICKEN:GGA.4459.2.S1_A_AT Ribosomal protein L35a gga
HG-U133_PLUS_2:215249_AT ribosomal protein L35a hs
HG-U133A_2:215249_AT ribosomal protein L35a hs
HG-U133A:215249_AT ribosomal protein L35a hs
HG-U133A_2:213687_S_AT ribosomal protein L35a hs
HG-U133_PLUS_2:213687_S_AT ribosomal protein L35a hs
HG-U133A:213687_S_AT ribosomal protein L35a hs
HUGENEFL:X52966_AT ribosomal protein L35a hs
U133_X3P:HS2.407059.1.S1_3P_S_AT ribosomal protein L35a hs
U133_X3P:HS.289093.0.A1_3P_X_AT ribosomal protein L35a hs
HG-U133_PLUS_2:225190_X_AT ribosomal protein L35a hs
HG-U133B:225190_X_AT ribosomal protein L35a hs
HU35KSUBC:RC_N70646_AT Ribosomal protein L35a hs
HG-U133_PLUS_2:215208_X_AT Ribosomal protein L35a hs
HG-U133A_2:215208_X_AT Ribosomal protein L35a hs
HG-U133A:215208_X_AT Ribosomal protein L35a hs
U133_X3P:HS.293287.0.A1_3P_AT Ribosomal protein L35a hs
U133_X3P:HS.289093.0.S1_3P_AT Ribosomal protein L35a hs
HG-U133_PLUS_2:238026_AT Ribosomal protein L35a hs
HG-U133B:238026_AT Ribosomal protein L35a hs
HG-U95C:53255_AT Ribosomal protein L35a hs
U133_X3P:HS.179666.3.A1_3P_AT Ribosomal protein L35a hs
U133_X3P:HS.179666.3.A1_3P_X_AT Ribosomal protein L35a hs
MOE430A:1417317_S_AT ribosomal protein L35a mm
MOUSE430A_2:1417317_S_AT ribosomal protein L35a mm
MOUSE430_2:1417317_S_AT ribosomal protein L35a mm
MG-U74AV2:93513_AT ribosomal protein L35a mm
MU19KSUBB:TC26624_F_AT Ribosomal protein L35a, mRNA (cDNA clone MGC:25278 IMAGE:4036717) mm
MU19KSUBB:TC33009_F_AT Ribosomal protein L35a, mRNA (cDNA clone MGC:25278 IMAGE:4036717) mm
RAE230A:1398760_AT ribosomal protein L35a rn
RAT230_2:1398760_AT ribosomal protein L35a rn
YEAST_2:1779816_AT Ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Ap and has similarity to rat L35a; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA82422 Ribosomal protein, large subunit protein 33 [Caenorhabditis elegans] ref|NP_495468.1| Ribosomal Protein, Large subunit family member (rpl-33) [Caenorhabditis elegans] sp|P49180|RL35A_CAEEL 60S ribosomal protein L35a 9.0E-67
blast CAE67541 Hypothetical protein CBG13066 [Caenorhabditis briggsae] 1.0E-62
blast AAA82422 Ribosomal protein, large subunit protein 33 [Caenorhabditis elegans] ref|NP_495468.1| Ribosomal Protein, Large subunit family member (rpl-33) [Caenorhabditis elegans] sp|P49180|RL35A_CAEEL 60S ribosomal protein L35a 1.0E-62
blast CAE67541 Hypothetical protein CBG13066 [Caenorhabditis briggsae] 2.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR001780 EMBL-EBI Ribosomal protein L35Ae 6.9E-74
Pfam IPR001780 EMBL-EBI Ribosomal protein L35Ae 2.8E-75
Sequence
Target Sequence 
>C. ELEGANS:193286_S_AT
gtctacttgtacaaggcccacaacaagactttgaaaaccggacacaccgtcgccacccgt
acccgtgctatctggggaaagatcactcgcccacacggaaacgccggagccgttagagcc
aagttccatcacaacatcccaccaagtgccctcggaaagagaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTACTTGTACAAGGCCCACAACA 472 469 193 Antisense
GTACAAGGCCCACAACAAGACTTTG 275 411 201 Antisense
GCCCACAACAAGACTTTGAAAACCG 642 285 208 Antisense
CCGTACCCGTGCTATCTGGGGAAAG 547 263 249 Antisense
CTGGGGAAAGATCACTCGCCCACAC 257 241 264 Antisense
CCGGAGCCGTTAGAGCCAAGTTCCA 424 255 296 Antisense
GGAGCCGTTAGAGCCAAGTTCCATC 22 491 298 Antisense
CCGTTAGAGCCAAGTTCCATCACAA 157 265 302 Antisense
TAGAGCCAAGTTCCATCACAACATC 277 653 306 Antisense
CCACCAAGTGCCCTCGGAAAGAGAA 507 273 331 Antisense
ACCAAGTGCCCTCGGAAAGAGAATC 674 83 333 Antisense
Sequence Source Affymetrix Proprietary Database