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[193273_at] NADH-ubiquinone oxidoreductase B8 subunit

Description C25A1.13 /REP_DB=WormBase Gene ID /WP=CE08379 /TR=O02220 /GB=CAB02765.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase B8 subunit
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 76      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 78      6grp_total > 66      4grp_total > 56      6grp_highest_sub > Da      6grp_high1_sub > Da      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1433
1 2 3 4 range
A 1289
(-3)
P 		2334
(1.4)
P 		2721.5
(3)
P 		1565.6
(-1.8)
P 		1433
B 1830.7
(-0.2)
P 		2136
(1.8)
P 		2315.4
(3)
P 		1420.2
(-3)
P 		895
C 1532.7
(-2.2)
P 		1941.9
(0)
P 		2477.3
(3)
P 		1396
(-3)
P 		1081
avr 1550.8
(-2.5) 		2137.3
(0.9) 		2504.7
(3) 		1460.6
(-3) 		1044

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1347
1 2 3 4 5 6 range
A 1261.2
(-2.7)
P 		1412.1
(-0.7)
P 		1557.3
(1.3)
P 		1240.2
(-3)
P 		1302.5
(-2.2)
P 		1680.9
(3)
P 		441
B 1250.5
(-3)
P 		1678
(1)
P 		1613.2
(0.4)
P 		1358.2
(-2)
P 		1425.9
(-1.4)
P 		1897
(3)
P 		647
C 1209.6
(-2.3)
P 		1498.7
(-1)
P 		1680.6
(-0.2)
P 		1446.7
(-1.2)
P 		1053
(-3)
P 		2400
(3)
P 		1347
avr 1240.4
(-3) 		1529.6
(-0.7) 		1617
(0) 		1348.4
(-2.1) 		1260.5
(-2.8) 		1992.6
(3) 		752
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 634
1 2 3 4 range
A 469.3
(-0)
P 		506.7
(0.5)
P 		711.4
(3)
P 		229.2
(-3)
P 		482
B 583.8
(3)
P 		527.5
(1.8)
P 		536.5
(2)
P 		302.6
(-3)
P 		281
C 507.4
(-0.4)
P 		558.3
(0.1)
P 		863.4
(3)
P 		235.4
(-3)
P 		628
avr 520.2
(0.5) 		530.8
(0.7) 		703.8
(3) 		255.7
(-3) 		448

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 571
1 2 3 4 5 6 range
A 232.5
(-3)
P 		502.2
(3)
P 		339.2
(-0.6)
P 		314.5
(-1.2)
P 		393
(0.6)
P 		409.2
(0.9)
P 		270
B 435.1
(-1.7)
P 		558.5
(0.4)
P 		391.7
(-2.4)
P 		359.7
(-3)
P 		579.3
(0.8)
P 		707.4
(3)
P 		348
C 448.4
(-1.2)
P 		457.3
(-1.1)
P 		427.7
(-1.4)
P 		290.9
(-3)
P 		340.7
(-2.4)
P 		803.4
(3)
P 		513
avr 372
(-2.1) 		506
(0.5) 		386.2
(-1.8) 		321.7
(-3) 		437.7
(-0.8) 		640
(3) 		318
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193273_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2511
Sequence Type Exemplar sequence
Representative Public ID C25A1.13 NCBI
Target Description C25A1.13 /REP_DB=WormBase Gene ID /WP=CE08379 /TR=O02220 /GB=CAB02765.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase B8 subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060285 NCBI Caenorhabditis elegans C25A1.13 (C25A1.13) mRNA, complete cds. 11/11 None
SNAP00000000661 ENSEMBL cdna:SNAP chromosome:CEL140:I:10201363:10202027:1 11/11 None
GENEFINDER00000000676 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10201363:10202027:1 11/11 None
C25A1.13 ENSEMBL cdna:known chromosome:CEL140:I:10201363:10202027:1 gene:C25A1.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10201293-10201958(+) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase B8 subunit
Ensembl C25A1.13
Entrez Gene 182876 Entrez gene
SwissProt O02220 EMBL-EBI
WormBase CE08379 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586827_AT similar to mitochondrial ribosomal protein L43 isoform a cfa
CANINE_2:CFA.10208.1.A1_AT similar to mitochondrial ribosomal protein L43 isoform a cfa
CANINE_2:CFAAFFX.15299.1.S1_AT similar to mitochondrial ribosomal protein L43 isoform a cfa
CANINE_2:CFAAFFX.15299.1.S1_S_AT similar to mitochondrial ribosomal protein L43 isoform a cfa
DROSOPHILA_2:1624480_AT mitochondrial ribosomal protein L43 dm
DROSGENOME1:147798_AT mitochondrial ribosomal protein L43 dm
HG-U133_PLUS_2:224332_S_AT mitochondrial ribosomal protein L43 hs
HG-U133B:224332_S_AT mitochondrial ribosomal protein L43 hs
HG-U133_PLUS_2:230027_S_AT mitochondrial ribosomal protein L43 hs
HG-U133B:230027_S_AT mitochondrial ribosomal protein L43 hs
HU35KSUBA:AA303626_AT mitochondrial ribosomal protein L43 hs
HU35KSUBD:RC_AA279811_S_AT mitochondrial ribosomal protein L43 hs
HU35KSUBD:RC_N26739_AT mitochondrial ribosomal protein L43 hs
HU35KSUBD:RC_AA476762_AT mitochondrial ribosomal protein L43 hs
U133_X3P:G13559403_3P_S_AT mitochondrial ribosomal protein L43 hs
U133_X3P:HS.151945.1.S1_3P_AT mitochondrial ribosomal protein L43 hs
HG-U133_PLUS_2:230026_AT mitochondrial ribosomal protein L43 hs
HG-U133B:230026_AT mitochondrial ribosomal protein L43 hs
HG-U95B:43824_AT mitochondrial ribosomal protein L43 hs
HG-U95B:57043_AT mitochondrial ribosomal protein L43 hs
HG-U95D:80471_AT mitochondrial ribosomal protein L43 hs
MG-U74AV2:99674_AT mitochondrial ribosomal protein L43 mm
MOE430A:1415681_AT mitochondrial ribosomal protein L43 mm
MOE430B:1415681_AT mitochondrial ribosomal protein L43 mm
MOUSE430A_2:1415681_AT mitochondrial ribosomal protein L43 mm
MOUSE430_2:1415681_AT mitochondrial ribosomal protein L43 mm
MU19KSUBB:TC33173_AT mitochondrial ribosomal protein L43 mm
MU19KSUBB:TC33174_AT Mitochondrial ribosomal protein L43 (Mrpl43), nuclear gene encoding mitochondrial protein, mRNA mm
MU19KSUBB:TC33174_G_AT Mitochondrial ribosomal protein L43 (Mrpl43), nuclear gene encoding mitochondrial protein, mRNA mm
RG-U34B:RC_AA997184_AT mitochondrial ribosomal protein L43 (predicted) rn
RAE230A:1367546_AT mitochondrial ribosomal protein L43 (predicted) rn
RAE230B:1367546_AT mitochondrial ribosomal protein L43 (predicted) rn
RAT230_2:1367546_AT mitochondrial ribosomal protein L43 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAB02765 Hypothetical protein C25A1.13 [Caenorhabditis elegans] ref|NP_492686.1| C25A1.13 [Caenorhabditis elegans] 1.0E-105
blast CAE66848 Hypothetical protein CBG12220 [Caenorhabditis briggsae] 1.0E-100
blast EAA01975 ENSANGP00000000132 [Anopheles gambiae str. PEST] ref|XP_306831.2| ENSANGP00000000132 [Anopheles gambiae str. PEST] 1.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR007741 EMBL-EBI Mitochondrial ribosome 2.0E-44
Sequence
Target Sequence 
>C. ELEGANS:193273_AT
agctctgaactttggatggcgattttctgattttctgaaaattccggcttataatggaat
ctcgcgttacaccaaccaacttcatcgcatcactgtcaggttttgcaaacaaagtgaatc
cagtgtcggagttcgcaattttattgagaatcaactcgttgaaatcggccgtaaaaaccc
atcagttgtgatctatgcccagcctgtgcgaaactcgaacccatcaatccgcgcagaata
tggaaatggtcggactctacagatcaatgcaaaaaacatgacacaagacgaagtgagcaa
agatgtgcatttgttattctcaagaagcggcgaaccagttgtgaagctagagagtcgtca
aagtgcattggttccttctattcaaggtcaatggacaccgataacatggcttccaacaga
aatgaacacggaacaacttccagcgaaacagttctcaaagcacaagtcaacggcaacgtc
ggcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTCTGAACTTTGGATGGCGATTT 181 81 66 Antisense
AATGGAATCTCGCGTTACACCAACC 158 161 118 Antisense
AACTTCATCGCATCACTGTCAGGTT 34 139 143 Antisense
GTGAATCCAGTGTCGGAGTTCGCAA 615 481 179 Antisense
GAAATCGGCCGTAAAAACCCATCAG 564 361 226 Antisense
TCAGTTGTGATCTATGCCCAGCCTG 655 621 247 Antisense
GCGAAACTCGAACCCATCAATCCGC 60 289 273 Antisense
ATGGTCGGACTCTACAGATCAATGC 213 51 311 Antisense
AAGTGCATTGGTTCCTTCTATTCAA 503 155 426 Antisense
GACACCGATAACATGGCTTCCAACA 271 367 459 Antisense
GCACAAGTCAACGGCAACGTCGGCG 189 317 525 Antisense
Sequence Source Affymetrix Proprietary Database