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[193270_at] O.volvulus antigen peptide like

Description F15B9.2 /REP_DB=WormBase Gene ID /WP=CE09407 /GEN=far-4 /TR=Q19477 /GB=CAB01421.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=O.volvulus antigen peptide like
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 12      6grp_highest_nonorm > 70      6grp_total > 23      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 53.9
(3)
P 		38
(1)
A 		5.1
(-3)
A 		39.8
(1.3)
A 		49
B 66.2
(3)
P 		5.5
(-3)
A 		21.4
(-1.4)
A 		39.7
(0.4)
A 		61
C 79.3
(2.6)
P 		46.7
(-0.1)
A 		11.2
(-3)
A 		83.6
(3)
P 		72
avr 66.5
(3) 		30.1
(-1.1) 		12.6
(-3) 		54.4
(1.7) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 425
1 2 3 4 5 6 range
A 451.1
(2)
P 		362.6
(0.8)
P 		520.6
(3)
P 		284
(-0.3)
P 		108.7
(-2.8)
P 		96.1
(-3)
P 		425
B 368.4
(2.8)
P 		378.4
(3)
P 		221.1
(-0.4)
P 		200.4
(-0.8)
P 		100.3
(-2.9)
P 		97.6
(-3)
P 		281
C 471.9
(3)
P 		331.4
(0.6)
P 		316.7
(0.3)
P 		233.4
(-1.1)
P 		134.9
(-2.8)
P 		120.9
(-3)
P 		351
avr 430.5
(3) 		357.5
(1.7) 		352.8
(1.6) 		239.3
(-0.5) 		114.6
(-2.8) 		104.9
(-3) 		326
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 19.6
(3)
P 		8.3
(-0.7)
A 		1.3
(-3)
A 		5.8
(-1.5)
A 		18
B 21.1
(3)
P 		1.4
(-3)
A 		5
(-1.9)
A 		8.5
(-0.8)
A 		20
C 26.2
(3)
P 		13.4
(-0.4)
A 		3.9
(-3)
A 		14.1
(-0.3)
P 		22
avr 22.3
(3) 		7.7
(-1.6) 		3.4
(-3) 		9.5
(-1.1) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 83.2
(0.4)
P 		129
(3)
P 		113.4
(2.1)
P 		72
(-0.2)
P 		32.8
(-2.5)
P 		23.4
(-3)
P 		106
B 128.2
(3)
P 		126
(2.9)
P 		53.7
(-1.9)
P 		53.1
(-1.9)
P 		40.7
(-2.7)
P 		36.4
(-3)
P 		92
C 174.9
(3)
P 		101.1
(-0.3)
P 		80.6
(-1.2)
P 		46.9
(-2.7)
P 		43.6
(-2.9)
P 		40.5
(-3)
P 		134
avr 128.8
(3) 		118.7
(2.4) 		82.6
(0.1) 		57.3
(-1.5) 		39
(-2.6) 		33.4
(-3) 		95
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193270_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14736
Sequence Type Exemplar sequence
Representative Public ID F15B9.2 NCBI
Target Description F15B9.2 /REP_DB=WormBase Gene ID /WP=CE09407 /GEN=far-4 /TR=Q19477 /GB=CAB01421.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=O.volvulus antigen peptide like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073851(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073851 NCBI Caenorhabditis elegans Fatty Acid/Retinol binding protein family member (far-4) (far-4) mRNA, complete cds. 11/11 None
SNAP00000032632 ENSEMBL cdna:SNAP chromosome:CEL140:V:13001102:13002240:-1 11/11 None
GENEFINDER00000032644 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13001102:13002240:-1 11/11 None
F15B9.2 ENSEMBL cdna:known chromosome:CEL140:V:13001102:13002240:-1 gene:F15B9.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13001101-13002240(-) 95.15 100.0
Public Domain and Genome References
Gene Title O.volvulus antigen peptide like
Gene Symbol far-4
Ensembl F15B9.2
Entrez Gene 191633 Entrez gene
SwissProt Q19477 EMBL-EBI
WormBase CE09407 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01421 Hypothetical protein F15B9.2 [Caenorhabditis elegans] ref|NP_506252.1| Fatty Acid/Retinol binding protein family member (far-4) [Caenorhabditis elegans] 6.0E-90
blast CAE64697 Hypothetical protein CBG09480 [Caenorhabditis briggsae] 1.0E-52
blast CAE64698 Hypothetical protein CBG09481 [Caenorhabditis briggsae] 5.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR008632 EMBL-EBI Nematode fatty acid retinoid binding 1.7E-91
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506252.1 2 4-21,175-197
F15B9.2 2 4-21,175-197
SNAP00000032632 2 4-21,175-197
GENEFINDER00000032644 2 4-21,175-197
Sequence
Target Sequence 
>C. ELEGANS:193270_AT
ttacttctacttgtactttctcttctcttttttattacatcagcttttccattcggagaa
ccacaagcgggaggatttcaaaaatttaagaacttacttccaagggagcttgtggaggcc
tacagtaacctgagtcaaaaagatcaacctgatctgaaagatgtattccgtaatcatcaa
aactaccgaaatgaacaagaaatggttaatgctttgaaaatgaaaaatccagcactcgga
gcccgaatggaacggagattgatggctttgaagcagaaaatcgatggattgagtagcgaa
gaagcgaaaggatttattcagaatttgatttcaactggaagacaaatttatgctcaacgg
cttaatggacaacaaatggatcagtcacaattgagacaagttggaatgggaattgcaatg
cactatcgatcattacctccatatgcacaacaggaacttcaaagtacttttccacaaatc
ttccaattcatgagacaaatgcgtgaacaacgtcttcgaagcatgatgggcggattcttt
ggcggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTACTTCTACTTGTACTTTCTCTTC 375 679 22 Antisense
TTATTACATCAGCTTTTCCATTCGG 213 679 53 Antisense
TTCCATTCGGAGAACCACAAGCGGG 88 699 68 Antisense
AAGGGAGCTTGTGGAGGCCTACAGT 441 157 123 Antisense
GGAGGCCTACAGTAACCTGAGTCAA 168 515 135 Antisense
TGAAAAATCCAGCACTCGGAGCCCG 421 569 242 Antisense
TATGCTCAACGGCTTAATGGACAAC 568 655 370 Antisense
ATGCACTATCGATCATTACCTCCAT 363 43 439 Antisense
GATCATTACCTCCATATGCACAACA 177 421 449 Antisense
ATGCGTGAACAACGTCTTCGAAGCA 247 41 520 Antisense
CATGATGGGCGGATTCTTTGGCGGC 29 209 543 Antisense
Sequence Source Affymetrix Proprietary Database