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[193269_s_at]

Description T04D3.4 /REP_DB=WormBase Gene ID /WP=CE13159 /GEN=gcy-35 /TR=O02298 /GB=CAB03288.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=guanylate cyclase beta subunit
Cluster 6grp_total > 25      4grp_total > 15      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 143.2
(2.5)
P 		74
(-1.4)
P 		45.2
(-3)
A 		152.4
(3)
P 		107
B 115.2
(3)
P 		37.9
(-3)
A 		53.8
(-1.8)
A 		105.9
(2.3)
P 		77
C 106.4
(3)
P 		43.5
(-3)
A 		59
(-1.5)
M 		100.5
(2.4)
P 		63
avr 121.6
(3) 		51.8
(-3) 		52.7
(-2.9) 		119.6
(2.8) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 91
1 2 3 4 5 6 range
A 113.7
(3)
P 		88.2
(1.3)
P 		70.6
(0.2)
P 		53.1
(-1)
P 		22.5
(-3)
A 		32.2
(-2.4)
A 		91
B 108.2
(3)
P 		99.4
(2.3)
P 		86.2
(1.4)
P 		28.2
(-3)
A 		41.5
(-2)
A 		57.8
(-0.8)
P 		80
C 100.5
(3)
P 		92.4
(2.3)
P 		48.8
(-1.6)
A 		77.1
(0.9)
P 		33.1
(-3)
A 		71.2
(0.4)
P 		67
avr 107.5
(3) 		93.3
(1.9) 		68.5
(-0.1) 		52.8
(-1.4) 		32.4
(-3) 		53.7
(-1.3) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 52.1
(3)
P 		16.1
(-2.4)
P 		11.8
(-3)
A 		22.3
(-1.4)
P 		40
B 36.7
(3)
P 		9.3
(-3)
A 		12.5
(-2.3)
A 		22.6
(-0.1)
P 		27
C 35.2
(3)
P 		12.5
(-3)
A 		20.6
(-0.9)
M 		16.9
(-1.8)
P 		23
avr 41.3
(3) 		12.6
(-3) 		15
(-2.5) 		20.6
(-1.3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 21
(0.5)
P 		31.4
(3)
P 		15.4
(-0.9)
P 		13.5
(-1.4)
P 		6.8
(-3)
A 		7.8
(-2.8)
A 		25
B 37.7
(3)
P 		33.1
(2.1)
P 		20.9
(-0.3)
P 		7.5
(-3)
A 		16.9
(-1.1)
A 		21.5
(-0.2)
P 		30
C 37.2
(3)
P 		28.2
(1)
P 		12.4
(-2.6)
A 		15.5
(-1.9)
P 		10.7
(-3)
A 		23.8
(-0)
P 		27
avr 32
(3) 		30.9
(2.7) 		16.2
(-1.6) 		12.2
(-2.8) 		11.5
(-3) 		17.7
(-1.2) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193269_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1727
Sequence Type Exemplar sequence
Representative Public ID T04D3.4 NCBI
Target Description T04D3.4 /REP_DB=WormBase Gene ID /WP=CE13159 /GEN=gcy-35 /TR=O02298 /GB=CAB03288.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=guanylate cyclase beta subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060943
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000016272 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13339737:13341562:1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_060943 7/11 Cross Hyb Matching Probes None
SNAP00000016265 7/11 Cross Hyb Matching Probes None
T04D3.4 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13333668-13341386(+) 99.91 99.91
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03288 Hypothetical protein T04D3.4 [Caenorhabditis elegans] gb|AAT73711.1| guanylate cyclase-like protein [Caenorhabditis elegans] ref|NP_493344.2| Guanylyl CYclase family member (gcy-35) [Caenorhabditis elegans] sp|O02298|GCY35_CAEEL Soluble guanylate cyclase gcy-35 1.0E-135
blast CAE73025 Hypothetical protein CBG20392 [Caenorhabditis briggsae] 1.0E-124
blast AAO65585 nitric oxide sensitive guanylyl cyclase beta2 subunit; B2PRO [Mus musculus] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.5E-70
Sequence
Target Sequence 
>C. ELEGANS:193269_S_AT
gacactgtcaacgtggcgaacaaaatggaatccaacggtattcaatgcaaaattcacgtc
agcgagactgggaagctcaacggactgaaagctaatccatcatacgtgttcattgacaga
ggaaatacggagatcaggggtaaaggaatgatgtacacctactttttggaacgaaacgat
cggaaaagtgtatgggagttgtgttcaagacctagatctggcgaacaaacaatcgacgga
tatatggagcttcacgatcaatcgatttatcaagaagaaggtggtcagcaggagaatctg
actgtggaaaatggaaattctgctcaaaataaccataataacaataataatacacatcat
tctggaaggaaattggtaagttctgcggcctacgcctgcctggcgcctacctcggcaatc
ggtaattaccgaagttaccgtacgccaaacaattttaaattactgctgtgcaaacaccaa
acaaaaattcagaaaacaggcggtaggcaggcaggcacttacgcctgcctaccagaaaac
ccatacctacaaaaactctttcagatgaatggatcaagtgtggatcccggcagtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACTGTCAACGTGGCGAACAAAA 582 365 1639 Antisense
GCAAAATTCACGTCAGCGAGACTGG 404 319 1685 Antisense
GCGAGACTGGGAAGCTCAACGGACT 206 289 1700 Antisense
AAGCTAATCCATCATACGTGTTCAT 513 149 1727 Antisense
GATGTACACCTACTTTTTGGAACGA 625 409 1788 Antisense
TATATGGAGCTTCACGATCAATCGA 122 653 1879 Antisense
GAAATTGGTAAGTTCTGCGGCCTAC 643 359 2007 Antisense
ATTACTGCTGTGCAAACACCAAACA 465 11 2097 Antisense
CGGTAGGCAGGCAGGCACTTACGCC 112 243 2139 Antisense
GGCAGGCACTTACGCCTGCCTACCA 158 537 2148 Antisense
GATCAAGTGTGGATCCCGGCAGTCA 249 421 2210 Antisense
Sequence Source Affymetrix Proprietary Database