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[193264_s_at] Queuine trna-ribosyltransferase

Description C13B4.2 /REP_DB=WormBase Gene ID /WP=CE15615 /GEN=tgt-1 /TR=O45248 /GB=CAB03876.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Queuine trna-ribosyltransferase
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 95      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 88      6grp_total > 86      4grp_total > 57      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 868
1 2 3 4 range
A 1191.6
(-2.2)
P 		1940.6
(3)
P 		1783.2
(1.9)
P 		1074.8
(-3)
P 		866
B 1117.9
(-3)
P 		1435.1
(-0.7)
P 		1942.9
(3)
P 		1117.1
(-3)
P 		826
C 1232.3
(-1.9)
P 		1577.6
(0.7)
P 		1879
(3)
P 		1090.2
(-3)
P 		789
avr 1180.6
(-2.3) 		1651.1
(1.3) 		1868.4
(3) 		1094
(-3) 		774

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 830
1 2 3 4 5 6 range
A 981.1
(-3)
P 		1117.1
(-1.8)
P 		1198.7
(-1.1)
P 		1292.8
(-0.3)
P 		1454.5
(1.1)
P 		1682.4
(3)
P 		701
B 985.8
(-3)
P 		1069.6
(-2.3)
P 		1310.4
(-0.1)
P 		1409.5
(0.7)
P 		1522.4
(1.7)
P 		1663.8
(3)
P 		678
C 974
(-3)
P 		1013.7
(-2.7)
P 		1424.8
(0.3)
P 		1333
(-0.4)
P 		1532.5
(1)
P 		1803.5
(3)
P 		830
avr 980.3
(-3) 		1066.8
(-2.3) 		1311.3
(-0.3) 		1345.1
(-0) 		1503.1
(1.3) 		1716.6
(3) 		736
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 498
1 2 3 4 range
A 433.8
(2.4)
P 		421.3
(2.1)
P 		466.2
(3)
P 		157.3
(-3)
P 		309
B 356.5
(0.3)
P 		354.4
(0.3)
P 		450.2
(3)
P 		238.1
(-3)
P 		212
C 407.9
(-0.1)
P 		453.6
(0.4)
P 		654.9
(3)
P 		183.8
(-3)
P 		471
avr 399.4
(0.7) 		409.8
(0.9) 		523.8
(3) 		193.1
(-3) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 440
1 2 3 4 5 6 range
A 180.9
(-3)
P 		397.3
(2)
P 		261.1
(-1.1)
P 		327.9
(0.4)
P 		438.9
(3)
P 		409.6
(2.3)
P 		258
B 343
(-2.5)
P 		356
(-2.2)
P 		318.2
(-3)
P 		373.3
(-1.9)
P 		618.5
(3)
P 		620.4
(3)
P 		302
C 361
(-1.3)
P 		309.3
(-2.3)
P 		362.6
(-1.3)
P 		268.1
(-3)
P 		495.9
(1.1)
P 		603.7
(3)
P 		336
avr 295
(-3) 		354.2
(-1.6) 		314
(-2.5) 		323.1
(-2.3) 		517.8
(2.4) 		544.6
(3) 		250
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193264_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3649
Sequence Type Exemplar sequence
Representative Public ID C13B4.2 NCBI
Target Description C13B4.2 /REP_DB=WormBase Gene ID /WP=CE15615 /GEN=tgt-1 /TR=O45248 /GB=CAB03876.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=Queuine trna-ribosyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064605(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064605 NCBI Caenorhabditis elegans Ubiquitin Specific Protease family member (usp-14) (usp-14) mRNA, complete cds. 11/11 None
C13B4.2.1 ENSEMBL cdna:known chromosome:CEL140:II:14485516:14488579:-1 gene:C13B4.2 11/11 None
C13B4.2.2 ENSEMBL cdna:known chromosome:CEL140:II:14485516:14488581:-1 gene:C13B4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14485724-14488570(-) 100.0 100.0
Public Domain and Genome References
Gene Title Queuine trna-ribosyltransferase
Gene Symbol usp-14
Ensembl C13B4.2
Entrez Gene 175105 Entrez gene
SwissProt Q17361 EMBL-EBI
WormBase CE15615 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256179_AT ubiquitin-specific protease 6, putative (UBP6) at
CANINE:1597438_AT similar to Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) cfa
CANINE_2:CFA.17913.1.S1_S_AT similar to Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) cfa
CANINE_2:CFA.3305.1.S1_AT similar to Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) cfa
CANINE_2:CFAAFFX.28036.1.S1_AT similar to Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) cfa
CANINE_2:CFAAFFX.28036.1.S1_S_AT similar to Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) cfa
DROSOPHILA_2:1631050_AT dm
DROSGENOME1:142863_AT dm
CHICKEN:GGA.16427.1.S1_AT similar to Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) gga
HG-U133A_2:201671_X_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-U133A:201671_X_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-U133_PLUS_2:201671_X_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-U133A_2:201672_S_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-U133A:201672_S_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-FOCUS:201672_S_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-U133_PLUS_2:201672_S_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HG-U95AV2:36982_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HU35KSUBA:HUM_ALU_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HU35KSUBB:HUM_ALU_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HU35KSUBC:HUM_ALU_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HU35KSUBD:HUM_ALU_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HUGENEFL:HUM_ALU_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HC-G110:HUM_ALU_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
HUGENEFL:U30888_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
U133_X3P:G4827049_3P_A_AT ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) hs
MOE430A:1416208_AT ubiquitin specific peptidase 14 mm
MOUSE430_2:1416208_AT ubiquitin specific peptidase 14 mm
MOUSE430A_2:1416208_AT ubiquitin specific peptidase 14 mm
MOUSE430_2:1455829_AT ubiquitin specific peptidase 14 mm
MOE430A:1455829_AT ubiquitin specific peptidase 14 mm
MOUSE430A_2:1455829_AT ubiquitin specific peptidase 14 mm
MU11KSUBA:AA277739_AT ubiquitin specific peptidase 14 mm
MG-U74AV2:96061_AT ubiquitin specific peptidase 14 mm
MG-U74AV2:162059_R_AT ubiquitin specific peptidase 14 mm
MOE430B:1439201_AT ubiquitin specific peptidase 14 mm
MOUSE430_2:1439201_AT ubiquitin specific peptidase 14 mm
MG-U74CV2:131445_AT Ubiquitin specific peptidase 14, mRNA (cDNA clone MGC:7106 IMAGE:3157723) mm
MOE430A:1437714_X_AT Ubiquitin specific peptidase 14, mRNA (cDNA clone MGC:7106 IMAGE:3157723) mm
MOUSE430A_2:1437714_X_AT Ubiquitin specific peptidase 14, mRNA (cDNA clone MGC:7106 IMAGE:3157723) mm
MOUSE430_2:1437714_X_AT Ubiquitin specific peptidase 14, mRNA (cDNA clone MGC:7106 IMAGE:3157723) mm
MU19KSUBA:TC16703_AT Ubiquitin specific peptidase 14, mRNA (cDNA clone MGC:7106 IMAGE:3157723) mm
MU19KSUBB:TC34528_AT Ubiquitin specific peptidase 14, mRNA (cDNA clone MGC:7106 IMAGE:3157723) mm
RG-U34B:RC_AA964368_AT ubiquitin specific protease 14 (predicted) rn
RAE230B:1383073_AT ubiquitin specific protease 14 (predicted) rn
RAT230_2:1383073_AT ubiquitin specific protease 14 (predicted) rn
RG-U34C:RC_AI071310_AT Ubiquitin specific protease 14 (predicted) rn
YEAST_2:1779703_AT Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05785 Hypothetical protein C13B4.2 [Caenorhabditis elegans] emb|CAB03876.1| Hypothetical protein C13B4.2 [Caenorhabditis elegans] ref|NP_497006.1| Ubiquitin Specific Protease family member (usp-14) [Caenorhabditis elegans] sp|Q17361|UBP14_CAEEL Ubiquitin carboxyl-terminal hydrolase 14 (Ubiquitin thiolesterase 14) (Ubiquitin-specific processing protease 14) (Deubiquitinating enzyme 14) 0.0
blast AAA74956 tRNA-guanine transglycosylase 0.0
blast CAE57931 Hypothetical protein CBG00984 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 0.014
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193264_S_AT
aaaatactcaaatctcgcgtcttccaaaatacctcactgtgaatatcaatcgattcttct
acaaggaaagcaccaagaccaacgcgaaaattctgaaatccgtccaattccccatgcaat
tagacacctatgacttgtgcagtcaggaactcaaggataaacttgttgctcgacgagctg
atatcaaattggaggaggatgcgaagctggagcgagagctgaggaagaaggtgttggaca
aagagcaaggcgataagattttcgatgatggagttgctctgcctaccgctttcgaagatg
atgcaggatcgaacaactctggattttatgatctcaagggaatcatcacccacaaaggcc
gatcctctcaagatggtcactacgtcgcctggatgcgatcatctgaagacggcaagtggc
gtctttttgacgatgagcatgtcactgttgtcgacgaggaagccattctgaaaacgtctg
gaggtggtgattggcacagtgcctacgttcttctctacgaggcccgtgtcatcaagcaat
tccctgaacttccaccagcaccagtaccgactgaagttgccgctgatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATACTCAAATCTCGCGTCTTCC 615 133 860 Antisense
CGTCTTCCAAAATACCTCACTGTGA 341 245 877 Antisense
CGTCCAATTCCCCATGCAATTAGAC 182 245 960 Antisense
CGATGATGGAGTTGCTCTGCCTACC 527 247 1122 Antisense
CTGCCTACCGCTTTCGAAGATGATG 629 235 1138 Antisense
CACAAAGGCCGATCCTCTCAAGATG 262 195 1210 Antisense
TCTCAAGATGGTCACTACGTCGCCT 35 587 1225 Antisense
TACGTCGCCTGGATGCGATCATCTG 398 645 1240 Antisense
ATTGGCACAGTGCCTACGTTCTTCT 614 3 1349 Antisense
ATCAAGCAATTCCCTGAACTTCCAC 354 27 1390 Antisense
TACCGACTGAAGTTGCCGCTGATAC 88 651 1424 Antisense
Sequence Source Affymetrix Proprietary Database