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[193263_at]

Description ZC410.4 /REP_DB=WormBase Gene ID /WP=CE03808 /GEN=twk-19 /TR=Q23297 /GB=CAA92568.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=potassium channel protein like
Cluster 6grp_high1_10_10 > 22      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_total > 22      4grp_total > 44      6grp_cgc > 62      6grp_high1_sub > De      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 159.1
(-1.6)
P 		231.5
(3)
P 		139.8
(-2.9)
P 		137.5
(-3)
P 		94
B 226.6
(3)
P 		165.7
(-0.6)
P 		124.4
(-3)
P 		136.7
(-2.3)
P 		102
C 186.5
(-0.3)
P 		203.2
(3)
P 		172.7
(-3)
P 		185.9
(-0.4)
P 		31
avr 190.7
(2) 		200.1
(3) 		145.6
(-3) 		153.4
(-2.1) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 107.4
(-1.8)
P 		201.8
(3)
P 		198.4
(2.8)
P 		123.4
(-1)
P 		83.7
(-3)
P 		88.5
(-2.8)
P 		118
B 239.8
(2.4)
P 		258.2
(3)
P 		217.9
(1.7)
P 		149.5
(-0.6)
P 		98.3
(-2.2)
P 		74.9
(-3)
P 		183
C 217.8
(2.1)
P 		241.5
(3)
P 		208.1
(1.7)
P 		207.2
(1.6)
P 		110
(-2.2)
P 		89.7
(-3)
M 		152
avr 188.3
(1.2) 		233.8
(3) 		208.1
(2) 		160
(0) 		97.3
(-2.5) 		84.4
(-3) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 57.9
(3)
P 		50.3
(1.8)
P 		36.5
(-0.4)
P 		20.1
(-3)
P 		38
B 72.3
(3)
P 		40.9
(-1.3)
P 		28.8
(-3)
P 		29.1
(-3)
P 		44
C 61.7
(3)
P 		58.4
(2.3)
P 		60.2
(2.7)
P 		31.3
(-3)
P 		30
avr 64
(3) 		49.9
(0.7) 		41.8
(-0.6) 		26.8
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 19.8
(-3)
P 		71.8
(3)
P 		43.2
(-0.3)
P 		31.3
(-1.7)
P 		25.3
(-2.4)
P 		21.6
(-2.8)
P 		52
B 83.4
(2.7)
P 		85.9
(3)
P 		52.9
(-0.4)
P 		39.6
(-1.8)
P 		39.9
(-1.8)
P 		27.9
(-3)
P 		58
C 80.7
(3)
P 		73.7
(2.2)
P 		53
(-0.3)
P 		41.7
(-1.6)
P 		35.6
(-2.3)
P 		30
(-3)
M 		51
avr 61.3
(1.1) 		77.1
(3) 		49.7
(-0.2) 		37.5
(-1.7) 		33.6
(-2.2) 		26.5
(-3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193263_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10222
Sequence Type Exemplar sequence
Representative Public ID ZC410.4 NCBI
Target Description ZC410.4 /REP_DB=WormBase Gene ID /WP=CE03808 /GEN=twk-19 /TR=Q23297 /GB=CAA92568.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=potassium channel protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028426(11), NM_001028425(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028426 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-8) (twk-8) mRNA, complete cds. 11/11 None
NM_001028425 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-8) (twk-8) mRNA, complete cds. 11/11 None
SNAP00000030925 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9082343:9086652:1 11/11 None
GENEFINDER00000030934 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9082343:9086953:1 11/11 None
ZC410.4b ENSEMBL cdna:known chromosome:CEL140:IV:9082343:9087524:1 gene:ZC410.4 11/11 None
ZC410.4a ENSEMBL cdna:known chromosome:CEL140:IV:9082343:9087489:1 gene:ZC410.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9082348-9087328(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZC410.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE46687 Hypothetical protein ZC410.4b [Caenorhabditis elegans] ref|NP_001023597.1| TWiK family of potassium channels family member (twk-8) [Caenorhabditis elegans] 0.0
blast CAA92568 Hypothetical protein ZC410.4a [Caenorhabditis elegans] ref|NP_001023596.1| TWiK family of potassium channels family member (twk-8) [Caenorhabditis elegans] 0.0
blast CAE74097 Hypothetical protein CBG21757 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023597.1 5 87-109,177-199,319-338,342-364,371-393
NP_001023596.1 5 87-109,177-199,319-338,342-364,371-393
ZC410.4b 5 87-109,177-199,319-338,342-364,371-393
ZC410.4a 5 87-109,177-199,319-338,342-364,371-393
SNAP00000030925 5 87-109,177-199,319-338,342-364,371-393
GENEFINDER00000030934 5 87-109,177-199,319-338,342-364,371-393
Sequence
Target Sequence 
>C. ELEGANS:193263_AT
tatagtcgactattcggaagctgatacaatgacccaaccagtgatagttaatgaatctga
tatgcaaacggattatgtgcagaccaaagaggaagaacttcaaacatatattgataaaaa
tgatatggaaacattgactgaaattgaaactaagaatgttcgaattcagacacctcagcc
tgtcattgaacaaaaatgcatacagactgaggatttgcatgaagaccgaaagtctccatc
aaaaatgtcatcagccaagaaaagactgcgtcgagcatttgcgggtaaatcaaaaccatc
tcgaaacagtgttgaacatccagtgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATAGTCGACTATTCGGAAGCTGAT 495 643 1752 Antisense
GGAAGCTGATACAATGACCCAACCA 107 531 1767 Antisense
ATACAATGACCCAACCAGTGATAGT 58 25 1775 Antisense
CAAACGGATTATGTGCAGACCAAAG 649 191 1816 Antisense
AATTCAGACACCTCAGCCTGTCATT 685 175 1914 Antisense
CACCTCAGCCTGTCATTGAACAAAA 632 201 1922 Antisense
GCATGAAGACCGAAAGTCTCCATCA 8 311 1968 Antisense
GTCTCCATCAAAAATGTCATCAGCC 396 471 1983 Antisense
AAGACTGCGTCGAGCATTTGCGGGT 590 151 2013 Antisense
AATCAAAACCATCTCGAAACAGTGT 122 169 2039 Antisense
GAAACAGTGTTGAACATCCAGTGAT 206 357 2054 Antisense
Sequence Source Affymetrix Proprietary Database