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[193255_at] 1-aminocyclopropane-1-carboxylate synthase

Description T04F3.1 /REP_DB=WormBase Gene ID /WP=CE06356 /TR=Q17464 /GB=CAA98419.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=1-aminocyclopropane-1-carboxylate synthase
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      4grp_highest_8_8 > 33      4grp_high_8_8 > 59      6grp_highest_nonorm > 83      6grp_total > 79      4grp_total > 61      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 401
(3)
P 		383.6
(2.6)
P 		373.1
(2.4)
P 		136.3
(-3)
P 		265
B 401.4
(1.7)
P 		387.6
(1.3)
P 		458.2
(3)
P 		204.6
(-3)
P 		254
C 440.7
(3)
P 		343.5
(-1.5)
P 		411.9
(1.7)
P 		310.7
(-3)
P 		130
avr 414.4
(3) 		371.6
(1.7) 		414.4
(3) 		217.2
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 348
1 2 3 4 5 6 range
A 391.2
(-0.9)
P 		564.6
(2.1)
P 		268.4
(-3)
P 		471.2
(0.5)
P 		409.6
(-0.6)
P 		616
(3)
P 		348
B 369.4
(-2.6)
P 		376.1
(-2.3)
P 		362.1
(-3)
P 		374.1
(-2.4)
P 		427.5
(0.2)
P 		483.5
(3)
P 		121
C 510
(3)
P 		392.2
(-2.6)
P 		498.6
(2.5)
P 		383
(-3)
P 		501
(2.6)
P 		456.2
(0.5)
P 		127
avr 423.5
(-1) 		444.3
(-0.1) 		376.4
(-3) 		409.4
(-1.6) 		446
(-0.1) 		518.6
(3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 146
(3)
P 		83.3
(0)
P 		97.5
(0.7)
P 		20
(-3)
P 		126
B 128
(3)
P 		95.7
(0.7)
P 		106.2
(1.5)
P 		43.6
(-3)
P 		84
C 145.9
(3)
P 		98.8
(-0)
P 		143.6
(2.9)
P 		52.4
(-3)
P 		94
avr 140
(3) 		92.6
(0.2) 		115.8
(1.6) 		38.7
(-3) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 72.1
(-2.4)
P 		200.8
(3)
P 		58.5
(-3)
P 		119.5
(-0.4)
P 		123.6
(-0.3)
P 		150
(0.9)
P 		142
B 128.5
(-0.4)
P 		125.2
(-0.6)
P 		87.9
(-3)
P 		99.1
(-2.3)
P 		173.7
(2.6)
P 		180.3
(3)
P 		92
C 189
(3)
P 		119.7
(-0.7)
P 		126.9
(-0.3)
P 		77
(-3)
P 		162.1
(1.6)
P 		152.7
(1.1)
P 		112
avr 129.9
(0.3) 		148.6
(1.9) 		91.1
(-3) 		98.5
(-2.4) 		153.1
(2.3) 		161
(3) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193255_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14282
Sequence Type Exemplar sequence
Representative Public ID T04F3.1 NCBI
Target Description T04F3.1 /REP_DB=WormBase Gene ID /WP=CE06356 /TR=Q17464 /GB=CAA98419.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=1-aminocyclopropane-1-carboxylate synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073562(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073562 NCBI Caenorhabditis elegans T04F3.1 (T04F3.1) mRNA, complete cds. 11/11 None
SNAP00000035116 ENSEMBL cdna:SNAP chromosome:CEL140:V:11751676:11756614:1 11/11 None
GENEFINDER00000035123 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11751753:11756614:1 11/11 None
T04F3.1 ENSEMBL cdna:known chromosome:CEL140:V:11745394:11756767:1 gene:T04F3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11745393-11756614(+) 100.0 100.0
Public Domain and Genome References
Gene Title 1-aminocyclopropane-1-carboxylate synthase
Ensembl T04F3.1
Entrez Gene 179608 Entrez gene
SwissProt Q17464 EMBL-EBI
WormBase CE31983 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16387_AT 1-aminocyclopropane-1-carboxylate synthase, putative / ACC synthase, putative at
ATH1-121501:253066_AT 1-aminocyclopropane-1-carboxylate synthase, putative / ACC synthase, putative at
MG-U74CV2:128663_AT RIKEN cDNA 2610203E10 gene mm
MOE430B:1429421_AT RIKEN cDNA 2610203E10 gene mm
MOUSE430_2:1429421_AT RIKEN cDNA 2610203E10 gene mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8483 transaminase activity inferred from electronic annotation QuickGO AmiGO
16847 1-aminocyclopropane-1-carboxylate synthase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98419 Hypothetical protein T04F3.1 [Caenorhabditis elegans] emb|CAA96672.2| Hypothetical protein T04F3.1 [Caenorhabditis elegans] ref|NP_505963.2| T04F3.1 [Caenorhabditis elegans] 0.0
blast CAE75307 Hypothetical protein CBG23277 [Caenorhabditis briggsae] 0.0
blast AAH83866 RGD1309314_predicted protein [Rattus norvegicus] 7.0E-44
blast AAH83866 RGD1309314_predicted protein [Rattus norvegicus] 2.0E-43
Protein Families
Method ID Description E-Value
ec 1A11_CUCMA (P23599) 1-aminocyclopropane-1-carboxylate synthase CMW33 (EC 4.4.1.14) (ACC synthase) (S-adenosyl-L-methionine methylthioadenosine-lyase) 1.0E-151
ec 1A11_CUCMA (P23599) 1-aminocyclopropane-1-carboxylate synthase CMW33 (EC 4.4.1.14) (ACC synthase) (S-adenosyl-L-methionine methylthioadenosine-lyase) 1.0E-159
ec 1A11_CUCMA (P23599) 1-aminocyclopropane-1-carboxylate synthase CMW33 (EC 4.4.1.14) (ACC synthase) (S-adenosyl-L-methionine methylthioadenosine-lyase) 1.0E-161
Protein Domains
Database ID Description E-Value
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 4.6E-9
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 4.6E-9
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 4.6E-9
Sequence
Target Sequence 
>C. ELEGANS:193255_AT
ttcaaagttccgaccattcctgtcttatcgtcatcgtctacaccgtcctgatagtgtcgc
atggttatggagtgttagcaaggattttggacttccaggactcaagtttgcagtgataca
tacaacgaatgaaggactttgtcaagcggcaacaaaactgcaaatgtactatccgtgctc
cccattcgttcaagattttgctgtgaacttattgtctgattcagaatggctacgagagtt
ccatcgtgaagtgaacaaacgtatttcaatccactatcgctacacttctgataatttgaa
acgtttggaaatcccatttattccagctcaagcaggaatatttgtttttgccgacttctc
caagcatcttacttcgttggattctgtaggagaacttgcattgtttgagcgtcttgctga
agcaggagtaatgctgactccgggtgttcatcagaaatgtcacgtgtttggatggttccg
cattgtgtttgcctgcacgaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAAAGTTCCGACCATTCCTGTCT 87 685 8874 Antisense
GTCTACACCGTCCTGATAGTGTCGC 203 469 8909 Antisense
CTGCAAATGTACTATCCGTGCTCCC 75 237 9031 Antisense
AACGTATTTCAATCCACTATCGCTA 590 143 9131 Antisense
TCCCATTTATTCCAGCTCAAGCAGG 409 597 9185 Antisense
GAATATTTGTTTTTGCCGACTTCTC 47 327 9209 Antisense
AAGCATCTTACTTCGTTGGATTCTG 371 151 9235 Antisense
GAACTTGCATTGTTTGAGCGTCTTG 42 351 9265 Antisense
AATGCTGACTCCGGGTGTTCATCAG 295 167 9303 Antisense
ATGTCACGTGTTTGGATGGTTCCGC 98 49 9330 Antisense
CGCATTGTGTTTGCCTGCACGAAAG 213 255 9352 Antisense
Sequence Source Affymetrix Proprietary Database