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[193247_at] FMRFamide neuropeptide repeats

Description W07E11.2 /REP_DB=WormBase Gene ID /WP=CE02373 /GEN=flp-3 /TR=Q23212 /GB=CAA90030.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=FMRFamide neuropeptide repeats
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 03      4grp_highest_8_8 > 46      4grp_high_8_8 > 11      6grp_highest_nonorm > 21      6grp_total > 24      4grp_total > 14      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2283
1 2 3 4 range
A 1688.3
(0.6)
P 		621.8
(-2.2)
P 		324.2
(-3)
P 		2601.2
(3)
P 		2277
B 1722.7
(1.3)
P 		518.1
(-2.4)
P 		318.4
(-3)
P 		2262.1
(3)
P 		1944
C 1677
(1.4)
P 		556.9
(-2.4)
P 		371.4
(-3)
P 		2144.2
(3)
P 		1773
avr 1696
(1.1) 		565.6
(-2.3) 		338
(-3) 		2335.8
(3) 		1998

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1225
1 2 3 4 5 6 range
A 1939
(3)
P 		1462.9
(0.7)
P 		1292.1
(-0.2)
P 		994.8
(-1.6)
P 		725.1
(-2.9)
P 		714.3
(-3)
P 		1225
B 1770.1
(3)
P 		1674.9
(2.4)
P 		1404.1
(0.8)
P 		1082.7
(-1.1)
P 		775.4
(-3)
P 		778.8
(-3)
P 		995
C 1597.6
(2)
P 		1769.9
(3)
P 		1207.6
(-0.2)
P 		1130.9
(-0.7)
P 		731.2
(-3)
P 		803.4
(-2.6)
P 		1039
avr 1768.9
(3) 		1635.9
(2.2) 		1301.3
(0.3) 		1069.5
(-1.1) 		743.9
(-3) 		765.5
(-2.9) 		1025
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 541
1 2 3 4 range
A 614.7
(3)
P 		135
(-2.4)
P 		84.8
(-3)
P 		380.8
(0.4)
P 		530
B 549.4
(3)
P 		128
(-2.3)
P 		73.8
(-3)
P 		482.1
(2.2)
P 		476
C 555.1
(3)
P 		160.1
(-2.6)
P 		129.5
(-3)
P 		361.5
(0.3)
P 		426
avr 573.1
(3) 		141
(-2.4) 		96
(-3) 		408.1
(0.9) 		477

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 442
1 2 3 4 5 6 range
A 357.4
(0.2)
P 		520.3
(3)
P 		281.4
(-1.1)
P 		252.3
(-1.6)
P 		218.8
(-2.2)
P 		173.9
(-3)
P 		346
B 615.9
(3)
P 		557.5
(1.9)
P 		341
(-2)
P 		286.8
(-3)
P 		315
(-2.5)
P 		290.4
(-2.9)
P 		329
C 592.2
(3)
P 		540
(2.1)
P 		307.3
(-1.7)
P 		227.4
(-3)
P 		236.6
(-2.8)
P 		268.9
(-2.3)
P 		365
avr 521.8
(2.6) 		539.3
(3) 		309.9
(-1.7) 		255.5
(-2.8) 		256.8
(-2.7) 		244.4
(-3) 		295
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193247_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16694
Sequence Type Exemplar sequence
Representative Public ID W07E11.2 NCBI
Target Description W07E11.2 /REP_DB=WormBase Gene ID /WP=CE02373 /GEN=flp-3 /TR=Q23212 /GB=CAA90030.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=FMRFamide neuropeptide repeats
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077293(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077293 NCBI Caenorhabditis elegans FMRF-Like Peptide family member (flp-3) (flp-3) mRNA, complete cds. 11/11 None
GENEFINDER00000023748 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10092148:10092847:-1 11/11 None
W07E11.2 ENSEMBL cdna:known chromosome:CEL140:X:10092148:10092893:-1 gene:W07E11.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000023744 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10092147-10092847(-) 100.0 100.0
Public Domain and Genome References
Gene Title FMRFamide neuropeptide repeats
Gene Symbol flp-3
Ensembl W07E11.2
Entrez Gene 181221 Entrez gene
SwissProt Q23212 EMBL-EBI
WormBase CE02373 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7218 neuropeptide signaling pathway inferred from electronic annotation QuickGO AmiGO
7218 neuropeptide signaling pathway inferred from sequence similarity QuickGO AmiGO
45759 negative regulation of action potential inferred from direct assay QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90030 Hypothetical protein W07E11.2 [Caenorhabditis elegans] ref|NP_509694.1| FMRF-Like Peptide family member (flp-3) [Caenorhabditis elegans] gb|AAC08940.1| FMRFamide-like peptide 3 [Caenorhabditis elegans] 1.0E-105
blast CAE58780 Hypothetical protein CBG01978 [Caenorhabditis briggsae] 1.0E-73
blast AAT76298 FMRFamide-related peptide FLP-18 precursor [Teladorsagia circumcincta] 9.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.23
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.48
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.24
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.51
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 1.4
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.54
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.16
Pfam IPR002544 EMBL-EBI FMRFamide-related peptide 0.24
Sequence
Target Sequence 
>C. ELEGANS:193247_AT
tcatcctgctcttctgtgtgaattgcgcgtttttggtagcgtcggatgccacaccaaaac
ggtctccactgggaacaatgcgctttggcaaacgagccattgctgatgaaatgacatttg
aggaggatggatactatccgtcaaatgtaatgtggaagcggtctactgttgattcttcgg
agcccgtcattcgtgatcagcgcactccattgggaactatgcgttttggaaaacgaagtg
cagagccattcggtactatgcgttttggaaaacgtaatcctgagaacgacacaccattcg
gaacaatgagatttggaaagcgtgctagtgaggatgctctgtttggaaccatgcgttttg
gaaagagagaagacggtaatgcgccatttggaacaatgaaatttgggaaacgtgaagcag
aagagccacttggaactatgcgctttggaaaaagatcagctgacgacagtgctccatttg
gcacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATCCTGCTCTTCTGTGTGAATTG 2 623 32 Antisense
GCGTTTTTGGTAGCGTCGGATGCCA 430 291 58 Antisense
TCGGATGCCACACCAAAACGGTCTC 378 603 73 Antisense
TCTCCACTGGGAACAATGCGCTTTG 648 609 94 Antisense
CGGTCTACTGTTGATTCTTCGGAGC 573 243 190 Antisense
TGATCAGCGCACTCCATTGGGAACT 521 565 225 Antisense
GAGCCATTCGGTACTATGCGTTTTG 391 385 274 Antisense
GTAATCCTGAGAACGACACACCATT 607 461 305 Antisense
GAGGATGCTCTGTTTGGAACCATGC 533 403 361 Antisense
GAGCCACTTGGAACTATGCGCTTTG 392 385 454 Antisense
GACGACAGTGCTCCATTTGGCACAA 581 379 493 Antisense
Sequence Source Affymetrix Proprietary Database