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[193238_s_at]

Description C48A7.1 /REP_DB=WormBase Gene ID /WP=CE25821 /GEN=egl-19 /TR=Q18698 /GB=AAB03158.3 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=calcium channel alpha subunit
Cluster 6grp_total > 14      4grp_total > 46      6grp_cgc > 41      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 346.5
(3)
P 		144.2
(-1.6)
A 		144.2
(-1.6)
A 		85.1
(-3)
A 		261
B 308
(3)
P 		134.8
(-1.5)
A 		103.3
(-2.3)
A 		77.7
(-3)
A 		230
C 319.2
(3)
P 		171.9
(-1.4)
A 		153.6
(-1.9)
A 		118.4
(-3)
A 		201
avr 324.6
(3) 		150.3
(-1.5) 		133.7
(-2) 		93.7
(-3) 		231

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 280
(2.3)
A 		303.3
(3)
P 		126.5
(-2.6)
A 		164.8
(-1.4)
A 		113.3
(-3)
A 		117.3
(-2.9)
A 		190
B 248.4
(2.4)
M 		265.3
(3)
P 		125.4
(-1.6)
A 		171.4
(-0.1)
A 		115.9
(-2)
A 		84.7
(-3)
A 		181
C 353.5
(3)
P 		304.6
(1.9)
P 		244.2
(0.5)
A 		216.4
(-0.2)
A 		128.2
(-2.2)
A 		95.1
(-3)
A 		258
avr 294
(3) 		291.1
(2.9) 		165.4
(-1) 		184.2
(-0.4) 		119.1
(-2.4) 		99
(-3) 		195
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 126.2
(3)
P 		31.3
(-2)
A 		37.7
(-1.7)
A 		12.5
(-3)
A 		114
B 98.2
(3)
P 		33.3
(-1.8)
A 		23.9
(-2.5)
A 		16.6
(-3)
A 		82
C 105.7
(3)
P 		49.4
(-0.9)
A 		53.5
(-0.7)
A 		20
(-3)
A 		86
avr 110
(3) 		38
(-1.6) 		38.4
(-1.6) 		16.4
(-3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 51.6
(-1.2)
A 		107.9
(3)
P 		27.5
(-3)
A 		41.8
(-1.9)
A 		34.2
(-2.5)
A 		28.6
(-2.9)
A 		80
B 86.4
(2.8)
M 		88.3
(3)
P 		30.5
(-3)
A 		45.4
(-1.5)
A 		47.1
(-1.3)
A 		31.6
(-2.9)
A 		58
C 131
(3)
P 		92.9
(0.7)
P 		62.2
(-1.2)
A 		43.5
(-2.3)
A 		41.5
(-2.4)
A 		31.8
(-3)
A 		99
avr 89.7
(2.4) 		96.4
(3) 		40.1
(-2.1) 		43.6
(-1.8) 		40.9
(-2.1) 		30.7
(-3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193238_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9846
Sequence Type Exemplar sequence
Representative Public ID C48A7.1 NCBI
Target Description C48A7.1 /REP_DB=WormBase Gene ID /WP=CE25821 /GEN=egl-19 /TR=Q18698 /GB=AAB03158.3 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=calcium channel alpha subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171379(11), NM_001027908(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171379 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-19) (egl-19) mRNA, complete cds. 11/11 A 
NM_001027908 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-19) (egl-19) mRNA, complete cds. 10/11 None
C48A7.1a ENSEMBL cdna:known chromosome:CEL140:IV:7405533:7418717:1 gene:C48A7.1 11/11 A 
C48A7.1b ENSEMBL cdna:known chromosome:CEL140:IV:7405566:7417917:1 gene:C48A7.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020638 3/11 Cross Hyb Matching Probes None
GENEFINDER00000020651 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7406113-7417899(+) 97.98 99.98
Public Domain and Genome References
Ensembl C48A7.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB03158 Egg laying defective protein 19, isoform a [Caenorhabditis elegans] ref|NP_741442.1| EGg Laying defective family member (egl-19) [Caenorhabditis elegans] gb|AAC47755.1| putative L-type calcium channel alpha 1 subunit [Caenorhabditis elegans] 0.0
blast CAE61874 Hypothetical protein CBG05858 [Caenorhabditis briggsae] 0.0
blast AAM75372 Egg laying defective protein 19, isoform b [Caenorhabditis elegans] ref|NP_001023079.1| EGg Laying defective family member (egl-19) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 3.3E-73
Pfam IPR005821 EMBL-EBI Ion transport protein 2.5E-53
Pfam IPR005821 EMBL-EBI Ion transport protein 1.0E-65
Pfam IPR005821 EMBL-EBI Ion transport protein 6.7E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741442.1 18 89-108,128-150,157-176,221-243,313-335,345-367,475-492,507-529,600-622,672-694,794-816,836-858,927-949,1043-1065,1110-1132,1152-1171,1246-1268,1341-1363
NP_001023079.1 19 89-108,128-150,157-176,221-243,313-335,345-367,475-492,507-529,600-622,672-694,794-816,836-858,927-949,1043-1065,1110-1132,1147-1169,1181-1198,1245-1264,1342-1364
C48A7.1a 18 89-108,128-150,157-176,221-243,313-335,345-367,475-492,507-529,600-622,672-694,794-816,836-858,927-949,1043-1065,1110-1132,1152-1171,1246-1268,1341-1363
C48A7.1b 19 89-108,128-150,157-176,221-243,313-335,345-367,475-492,507-529,600-622,672-694,794-816,836-858,927-949,1043-1065,1110-1132,1147-1169,1181-1198,1245-1264,1342-1364
Sequence
Target Sequence 
>C. ELEGANS:193238_S_AT
acagaagaccacactctcttttcaacaatttggtacatcgtctatctggtgccggatcca
aatctcctactgaacacgaacgtatagaaaagggctctacacttttgccattccaaccac
gctctttctctccaactcattcacttgctggcgctgaaggatcaccagtaccatcacaaa
tgcatcgtggagctccaatcaatcaatcaataaatcttccacctgtcaatggatcagctc
gacgtcttcccgcccttccgccgtacgcaaatcatattcacgatgagacggacgacggcc
ccaggtatcgggatactggcgatcgagctggttacgatcaatcaagcagaatggttgttg
ccaatagaaagtacgccgacgctcggattgtcggagccgctcgacgagaaattgaggaag
catattcacttggtgaacaggaaattgacttggcagcagactcattagctccattaatgc
aacatgttggaatgcatgatatcagagatatcaatgaaaactctcgaagcgcacttttac
gacccgccgaaaattcatctcggcagcatgattcacgcggagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGAAGACCACACTCTCTTTTCAA 60 109 4850 Antisense
TTTGGTACATCGTCTATCTGGTGCC 189 665 4878 Antisense
TATCTGGTGCCGGATCCAAATCTCC 435 657 4892 Antisense
CTCATTCACTTGCTGGCGCTGAAGG 271 229 4985 Antisense
ATCAATAAATCTTCCACCTGTCAAT 109 27 5055 Antisense
CTTCCGCCGTACGCAAATCATATTC 402 219 5104 Antisense
GGATACTGGCGATCGAGCTGGTTAC 604 509 5160 Antisense
AAAGTACGCCGACGCTCGGATTGTC 460 121 5217 Antisense
GGCAGCAGACTCATTAGCTCCATTA 703 535 5301 Antisense
ACTCTCGAAGCGCACTTTTACGACC 330 97 5369 Antisense
TCGGCAGCATGATTCACGCGGAGGA 128 607 5409 Antisense
Sequence Source Affymetrix Proprietary Database