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[193230_at]

Description F28B12.2 /REP_DB=WormBase Gene ID /WP=CE27143 /GEN=egl-44 /GB=AAK31508.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Transcriptional enhancer factor (TEF-1)
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 16      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 60      6grp_total > 15      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 436
1 2 3 4 range
A 618.4
(3)
P 		453.2
(-0.1)
P 		331
(-2.5)
P 		302.6
(-3)
P 		316
B 547.8
(3)
P 		343.5
(-1.1)
P 		333.4
(-1.3)
P 		250.8
(-3)
P 		297
C 631.7
(3)
P 		414.8
(0)
P 		350.2
(-0.9)
P 		195.7
(-3)
P 		436
avr 599.3
(3) 		403.8
(-0.4) 		338.2
(-1.5) 		249.7
(-3) 		350

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 447
1 2 3 4 5 6 range
A 259
(-3)
P 		482.5
(3)
P 		315.6
(-1.5)
P 		356.4
(-0.4)
P 		271.2
(-2.7)
P 		353.1
(-0.5)
P 		224
B 404.3
(2.8)
P 		407.8
(3)
P 		354.3
(0.7)
P 		313.5
(-1.1)
P 		268.7
(-3)
P 		269.4
(-3)
P 		139
C 706.1
(3)
P 		362.8
(-1.8)
P 		387
(-1.5)
P 		357.3
(-1.9)
P 		366.6
(-1.8)
P 		281
(-3)
P 		425
avr 456.5
(3) 		417.7
(1.5) 		352.3
(-1) 		342.4
(-1.4) 		302.2
(-3) 		301.2
(-3) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 225.2
(3)
P 		98.4
(-1.2)
P 		86.5
(-1.6)
P 		44.3
(-3)
P 		181
B 174.7
(3)
P 		84.8
(-1.4)
P 		77.3
(-1.8)
P 		53.4
(-3)
P 		121
C 209.1
(3)
P 		119.2
(-0.1)
P 		122.1
(0)
P 		33
(-3)
P 		176
avr 203
(3) 		100.8
(-0.8) 		95.3
(-1.1) 		43.6
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 47.7
(-3)
P 		171.6
(3)
P 		68.7
(-2)
P 		90.4
(-0.9)
P 		81.8
(-1.3)
P 		86
(-1.1)
P 		124
B 140.7
(3)
P 		135.7
(2.5)
P 		86
(-2.7)
P 		83
(-3)
P 		109.2
(-0.3)
P 		100.5
(-1.2)
P 		58
C 261.7
(3)
P 		110.7
(-1.8)
P 		98.5
(-2.2)
P 		71.8
(-3)
P 		118.6
(-1.5)
P 		94.1
(-2.3)
P 		190
avr 150
(3) 		139.3
(2.1) 		84.4
(-2.8) 		81.7
(-3) 		103.2
(-1.1) 		93.5
(-2) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193230_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4658
Sequence Type Exemplar sequence
Representative Public ID F28B12.2 NCBI
Target Description F28B12.2 /REP_DB=WormBase Gene ID /WP=CE27143 /GEN=egl-44 /GB=AAK31508.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Transcriptional enhancer factor (TEF-1)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171850(11), NM_171007(11), NM_062785(11), NM_182106(11), NM_182104(11), NM_182105(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171850 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-44) (egl-44) mRNA, complete cds. 11/11 None
NM_171007 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-44) (egl-44) mRNA, complete cds. 11/11 None
NM_062785 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-44) (egl-44) mRNA, complete cds. 11/11 None
NM_182106 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-44) (egl-44) mRNA, complete cds. 11/11 None
NM_182104 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-44) (egl-44) mRNA, complete cds. 11/11 None
NM_182105 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-44) (egl-44) mRNA, complete cds. 10/11 None
SNAP00000017907 ENSEMBL cdna:SNAP chromosome:CEL140:II:5938986:5940189:1 10/11 None
GENEFINDER00000017911 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5936403:5940189:1 11/11 None
F28B12.2a ENSEMBL cdna:known chromosome:CEL140:II:5933023:5940762:1 gene:F28B12.2 11/11 A 
F28B12.2c ENSEMBL cdna:known chromosome:CEL140:II:5936403:5940189:1 gene:F28B12.2 11/11 None
F28B12.2b ENSEMBL cdna:known chromosome:CEL140:II:5936403:5940189:1 gene:F28B12.2 11/11 None
F28B12.2e.2 ENSEMBL cdna:known chromosome:CEL140:II:5936404:5940189:1 gene:F28B12.2 9/11 None
F28B12.2e.1 ENSEMBL cdna:known chromosome:CEL140:II:5936404:5940255:1 gene:F28B12.2 10/11 None
F28B12.2f ENSEMBL cdna:known chromosome:CEL140:II:5936408:5940189:1 gene:F28B12.2 11/11 None
F28B12.2d ENSEMBL cdna:known chromosome:CEL140:II:5937207:5940189:1 gene:F28B12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5936165-5940186(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28B12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31508 Egg laying defective protein 44, isoform a [Caenorhabditis elegans] ref|NP_741007.1| EGg Laying defective family member (egl-44) [Caenorhabditis elegans] sp|Q19849|EGL44_CAEEL Transcription enhancer factor-like protein egl-44 (Egg laying defective protein 44) 0.0
blast AAO38647 Egg laying defective protein 44, isoform c [Caenorhabditis elegans] ref|NP_495186.2| EGg Laying defective family member (egl-44) [Caenorhabditis elegans] 0.0
blast AAO38646 Egg laying defective protein 44, isoform b [Caenorhabditis elegans] ref|NP_741006.2| EGg Laying defective family member (egl-44) [Caenorhabditis elegans] 0.0
blast T16195 hypothetical protein F28B12.2 - Caenorhabditis elegans 0.0
blast AAO38650 Egg laying defective protein 44, isoform f [Caenorhabditis elegans] ref|NP_871906.1| EGg Laying defective family member (egl-44) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000818 EMBL-EBI TEA/ATTS 7.9E-92
Pfam IPR000818 EMBL-EBI TEA/ATTS 2.0E-52
Pfam IPR000818 EMBL-EBI TEA/ATTS 1.0E-126
Pfam IPR000818 EMBL-EBI TEA/ATTS 1.0E-126
Pfam IPR000818 EMBL-EBI TEA/ATTS 1.0E-126
Pfam IPR000818 EMBL-EBI TEA/ATTS 1.0E-126
Pfam IPR000818 EMBL-EBI TEA/ATTS 1.0E-126
Pfam IPR000818 EMBL-EBI TEA/ATTS 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193230_AT
cgacaaaacagtttcacctcttctccgcatatgtcaaatgtaataagacaagtttaagga
cagaactcgtgaagattgagaatacactggaaaaagatgatattgacatttctgtatttt
acgaaaaatatccgaaattacttcgagaattgttcgagaaatccgagaaaaaggatgtct
tcttccttgcaaagtgttgggcaaatattaatgtttcggatgatgtacaaaactgtcaat
acgcagtcgactcgttctactcaagtcgtgaaaaattccaattaaaggtctcaacaatgg
cttgctcatttggaaaccaggcagttgaaaaaatagagcaatacttcccaatcgaattcg
atggatcatattcgttcatattgaacaactccccaatgtgtgactatatggttaagttca
ttgccgagctgaagaagctgaatgtaatagaaacaatgaacaacgtgctggagaatttta
cagttttacagattgtcacaaacagcgaaactgatgaattattaatggttctctgttttg
tatttgaagtatcacaagaaccagagccatcatgttctgtctacaggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACAAAACAGTTTCACCTCTTCTC 409 251 860 Antisense
CCTCTTCTCCGCATATGTCAAATGT 533 267 876 Antisense
ATGTCTTCTTCCTTGCAAAGTGTTG 662 47 1034 Antisense
GTCAATACGCAGTCGACTCGTTCTA 275 463 1094 Antisense
GACTCGTTCTACTCAAGTCGTGAAA 677 371 1108 Antisense
AAGGTCTCAACAATGGCTTGCTCAT 359 159 1144 Antisense
GGCTTGCTCATTTGGAAACCAGGCA 186 537 1158 Antisense
GAGCAATACTTCCCAATCGAATTCG 348 389 1195 Antisense
GAACAACTCCCCAATGTGTGACTAT 137 353 1242 Antisense
ATGGTTAAGTTCATTGCCGAGCTGA 686 45 1267 Antisense
GAGCCATCATGTTCTGTCTACAGGC 364 385 1423 Antisense
Sequence Source Affymetrix Proprietary Database