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[193227_at] potassium channel protein like

Description T01B4.2 /REP_DB=WormBase Gene ID /WP=CE06337 /GEN=twk-17 /TR=Q22043 /GB=CAA93876.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=potassium channel protein like
Cluster 6grp_total > 79      4grp_total > 18      6grp_cgc > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 40.4
(-3)
A 		105.1
(3)
A 		67.9
(-0.4)
A 		72.5
(-0)
A 		65
B 66.2
(-2.4)
A 		101.7
(-0.7)
A 		54.1
(-3)
A 		180
(3)
P 		126
C 90.4
(-2.8)
M 		88.3
(-3)
A 		89.1
(-2.9)
A 		158.4
(3)
A 		70
avr 65.7
(-3) 		98.4
(-0.2) 		70.4
(-2.6) 		137
(3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 75.2
(-1.2)
A 		78.1
(-0.8)
A 		64.1
(-3)
A 		75.2
(-1.2)
A 		101.8
(3)
A 		75.8
(-1.1)
A 		38
B 80.8
(-1.2)
A 		77.1
(-1.5)
A 		61.9
(-3)
A 		112.8
(1.9)
A 		63.4
(-2.9)
A 		123.7
(3)
P 		62
C 56.3
(-1.7)
A 		121.9
(3)
A 		38.5
(-3)
A 		62.8
(-1.3)
A 		107
(1.9)
P 		96.7
(1.2)
M 		83
avr 70.8
(-0.8) 		92.4
(2.1) 		54.8
(-3) 		83.6
(0.9) 		90.7
(1.9) 		98.7
(3) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 14.7
(-1)
A 		22.8
(3)
A 		17.7
(0.5)
A 		10.6
(-3)
A 		12
B 21.1
(-1)
A 		25.1
(-0.1)
A 		12.5
(-3)
A 		38.4
(3)
P 		26
C 29.9
(1.7)
M 		25.4
(-3)
A 		31.1
(3)
A 		26.7
(-1.6)
A 		6
avr 21.9
(-1.1) 		24.4
(2) 		20.4
(-3) 		25.2
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 13.9
(-3)
A 		27.8
(2)
A 		14
(-3)
A 		19.1
(-1.1)
A 		30.7
(3)
A 		18.5
(-1.4)
A 		17
B 28.1
(-0.5)
A 		25.7
(-0.9)
A 		15
(-3)
A 		29.9
(-0.1)
A 		25.7
(-0.9)
A 		46.1
(3)
P 		31
C 20.9
(-0.6)
A 		37.2
(3)
A 		9.8
(-3)
A 		12.6
(-2.4)
A 		34.6
(2.4)
P 		32.4
(1.9)
M 		27
avr 21
(-0.5) 		30.2
(2.4) 		12.9
(-3) 		20.5
(-0.6) 		30.3
(2.4) 		32.3
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193227_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17602
Sequence Type Exemplar sequence
Representative Public ID T01B4.2 NCBI
Target Description T01B4.2 /REP_DB=WormBase Gene ID /WP=CE06337 /GEN=twk-17 /TR=Q22043 /GB=CAA93876.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=potassium channel protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078254(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078254 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-22) (twk-22) mRNA, complete cds. 11/11 None
GENEFINDER00000021936 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16308354:16314542:1 11/11 None
SNAP00000021933 ENSEMBL cdna:SNAP chromosome:CEL140:X:16308850:16314542:1 11/11 None
T01B4.2 ENSEMBL cdna:known chromosome:CEL140:X:16308354:16314542:1 gene:T01B4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16308353-16314542(+) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein like
Gene Symbol twk-22
Ensembl T01B4.2
Entrez Gene 181703 Entrez gene
SwissProt Q22043 EMBL-EBI
WormBase CE06337 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93876 Hypothetical protein T01B4.2 [Caenorhabditis elegans] ref|NP_510655.1| TWiK family of potassium channels family member (twk-22) [Caenorhabditis elegans] 0.0
blast CAE69981 Hypothetical protein CBG16379 [Caenorhabditis briggsae] 1.0E-157
blast CAE69981 Hypothetical protein CBG16379 [Caenorhabditis briggsae] 1.0E-154
blast CAA93875 Hypothetical protein T01B4.1 [Caenorhabditis elegans] ref|NP_510654.1| TWiK family of potassium channels family member (twk-21) [Caenorhabditis elegans] 1.0E-138
blast CAE69981 Hypothetical protein CBG16379 [Caenorhabditis briggsae] 1.0E-135
blast CAA93875 Hypothetical protein T01B4.1 [Caenorhabditis elegans] ref|NP_510654.1| TWiK family of potassium channels family member (twk-21) [Caenorhabditis elegans] 1.0E-133
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510655.1 4 173-195,274-296,362-381,460-482
T01B4.2 4 173-195,274-296,362-381,460-482
GENEFINDER00000021936 6 173-195,269-286,296-318,380-399,404-424,431-453
SNAP00000021933 6 128-150,224-241,251-273,335-354,359-376,408-430
Sequence
Target Sequence 
>C. ELEGANS:193227_AT
tatggagaacatgcccgatcggccgattagtgaaagacggtttagtgtcttatcacctgg
agaagctccacaagctccaccgcctgtacttggcacatttatgcttcatagcctgtctgt
aaaaaagaagcttctcgcgagatcggaaagcattttgaatagaactggatcaccatggcc
acacgcagccgaaatgaatcgtccaaaagaggctaccaggttgggcgagccgtcggctct
ccgtgtgtccgcccccaacctgacgatatctggtggaaatcggtcgccgtctggcggcgc
gctatcagtgatcactgaggctagcgatgaagatacgaggcacttcaaaaagcaccgaag
accattggcaaagactgtctcgacagatgggacagtttcaaaccacggtagtgccgagag
cttggatcaagtgatggacgagttgcaactagagtctcacgaccaggctgtcacacccaa
accttatcgggatccaactacaagcaccaggttccgaaaacgagacgagacagttgtttc
gagagctgaaaaaatgcctctttccccgaatgaagtgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGGAGAACATGCCCGATCGGCCG 642 653 1671 Antisense
GACGGTTTAGTGTCTTATCACCTGG 359 377 1706 Antisense
CACCGCCTGTACTTGGCACATTTAT 586 199 1748 Antisense
TAGAACTGGATCACCATGGCCACAC 138 653 1830 Antisense
AGAGGCTACCAGGTTGGGCGAGCCG 127 57 1878 Antisense
CCCCCAACCTGACGATATCTGGTGG 474 261 1922 Antisense
TATCTGGTGGAAATCGGTCGCCGTC 325 653 1937 Antisense
GCAACTAGAGTCTCACGACCAGGCT 660 319 2115 Antisense
GTCACACCCAAACCTTATCGGGATC 123 463 2140 Antisense
ATCGGGATCCAACTACAAGCACCAG 602 35 2156 Antisense
GCCTCTTTCCCCGAATGAAGTGTTG 289 281 2226 Antisense
Sequence Source Affymetrix Proprietary Database