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[193223_at] potassium channel protein like

Description F21C3.1 /REP_DB=WormBase Gene ID /WP=CE05676 /GEN=twk-7 /TR=Q19671 /GB=CAA95801.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=potassium channel protein like
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 71      6grp_total > 70      4grp_total > 31      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 273
1 2 3 4 range
A 390.2
(2.1)
P 		159.9
(-3)
P 		247.8
(-1.1)
P 		433
(3)
P 		273
B 419.4
(3)
P 		210
(-3)
P 		221.9
(-2.7)
P 		249.3
(-1.9)
P 		209
C 400.9
(3)
P 		231.9
(-2.6)
P 		219.3
(-3)
P 		297.7
(-0.4)
P 		182
avr 403.5
(3) 		200.6
(-3) 		229.7
(-2.1) 		326.7
(0.7) 		203

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 403
1 2 3 4 5 6 range
A 173.1
(-3)
P 		235.9
(-1.5)
P 		416.4
(3)
P 		390.1
(2.4)
P 		316
(0.5)
P 		310.4
(0.4)
P 		243
B 229.3
(-3)
P 		256.5
(-2.5)
P 		321.5
(-1.4)
P 		575.6
(3)
P 		276.7
(-2.2)
P 		260.7
(-2.5)
P 		346
C 243.2
(-2.4)
P 		308.5
(0.3)
P 		289.7
(-0.5)
P 		374
(3)
P 		228.3
(-3)
P 		321.1
(0.8)
P 		146
avr 215.2
(-3) 		267
(-1.7) 		342.5
(0.3) 		446.6
(3) 		273.7
(-1.5) 		297.4
(-0.9) 		231
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 142.1
(3)
P 		34.7
(-3)
P 		64.8
(-1.3)
P 		63.4
(-1.4)
P 		107
B 133.7
(3)
P 		51.9
(-3)
P 		51.4
(-3)
P 		53.1
(-2.9)
P 		82
C 132.7
(3)
P 		66.7
(-1.8)
P 		76.4
(-1.1)
P 		50.2
(-3)
P 		83
avr 136.2
(3) 		51.1
(-3) 		64.2
(-2.1) 		55.6
(-2.7) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 31.9
(-3)
P 		83.9
(1.7)
P 		90.7
(2.3)
P 		98.9
(3)
P 		95.3
(2.7)
P 		75.6
(0.9)
P 		67
B 79.8
(-2.9)
P 		85.4
(-2.4)
P 		78.1
(-3)
P 		152.5
(3)
P 		112.4
(-0.2)
P 		97.2
(-1.5)
P 		74
C 90.1
(-0.1)
P 		94.1
(0.6)
P 		73.7
(-3)
P 		75.2
(-2.7)
P 		73.9
(-3)
P 		107.5
(3)
P 		34
avr 67.3
(-3) 		87.8
(-0) 		80.8
(-1.1) 		108.9
(3) 		93.9
(0.8) 		93.4
(0.8) 		42
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193223_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.759
Sequence Type Exemplar sequence
Representative Public ID F21C3.1 NCBI
Target Description F21C3.1 /REP_DB=WormBase Gene ID /WP=CE05676 /GEN=twk-7 /TR=Q19671 /GB=CAA95801.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=potassium channel protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059653(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059653 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-1) (twk-1) mRNA, complete cds. 11/11 None
SNAP00000007019 ENSEMBL cdna:SNAP chromosome:CEL140:I:7277489:7280304:-1 11/11 None
GENEFINDER00000007030 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7278091:7280304:-1 9/11 None
F21C3.1 ENSEMBL cdna:known chromosome:CEL140:I:7277363:7280304:-1 gene:F21C3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7277560-7280235(-) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein like
Gene Symbol twk-1
Ensembl F21C3.1
Entrez Gene 192072 Entrez gene
SwissProt Q19671 EMBL-EBI
WormBase CE32387 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA95801 Hypothetical protein F21C3.1 [Caenorhabditis elegans] ref|NP_492054.2| TWiK family of potassium channels family member (twk-1) [Caenorhabditis elegans] 0.0
blast CAE74226 Hypothetical protein CBG21910 [Caenorhabditis briggsae] 1.0E-165
blast CAE74226 Hypothetical protein CBG21910 [Caenorhabditis briggsae] 1.0E-154
blast CAE74226 Hypothetical protein CBG21910 [Caenorhabditis briggsae] 1.0E-144
blast CAB07286 Hypothetical protein T28A8.1 [Caenorhabditis elegans] ref|NP_499790.1| TWiK family of potassium channels family member (twk-40) [Caenorhabditis elegans] 3.0E-21
blast CAB07286 Hypothetical protein T28A8.1 [Caenorhabditis elegans] ref|NP_499790.1| TWiK family of potassium channels family member (twk-40) [Caenorhabditis elegans] 4.0E-21
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492054.2 4 64-86,133-155,183-205,215-237
F21C3.1 4 64-86,133-155,183-205,215-237
SNAP00000007019 6 67-89,102-124,131-153,177-199,204-221,231-253
GENEFINDER00000007030 6 33-55,70-92,99-121,145-167,172-189,199-221
Sequence
Target Sequence 
>C. ELEGANS:193223_AT
gatatggagattactccccaactccagaaggactttttcaatatataattgtaactgtat
acctttgcactggagttgctacaatgcttctattttttgcaagtcttcaaaaaggaatca
tgtggattcattactatggaagaaaggtttcagattcagaagaagctgaaatttggtttg
gagggcaaatgatgactgtcaaggatttggtcactttggtggctgaaaagtttggatcaa
cgccggaaaaactgagggaagtattacatgatcttgataagatattggaagttgcatgca
aacaagcagaggaagatgacgatgaagaagattggagatcagttaataattcggaagctt
tatctcctcctgctccaagaaagaagacagttttcttatcaggatcttccgatggagatg
attgtcaggcaacgattcattccttcacaagtactaatcagagaagtattccgaaggata
ctgagttggcaattcttgccctcggcactattcaacatcatttgaggaaaccatcagttc
gaggaatacacggtcaccacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATGGAGATTACTCCCCAACTCC 178 425 626 Antisense
TACTCCCCAACTCCAGAAGGACTTT 533 643 637 Antisense
GTAACTGTATACCTTTGCACTGGAG 627 461 676 Antisense
GAGTTGCTACAATGCTTCTATTTTT 622 397 698 Antisense
GGATTTGGTCACTTTGGTGGCTGAA 22 511 828 Antisense
TAATTCGGAAGCTTTATCTCCTCCT 147 635 972 Antisense
TCTCCTCCTGCTCCAAGAAAGAAGA 128 611 988 Antisense
GACAGTTTTCTTATCAGGATCTTCC 248 369 1011 Antisense
GTCAGGCAACGATTCATTCCTTCAC 203 467 1049 Antisense
CCTCGGCACTATTCAACATCATTTG 539 267 1125 Antisense
GTTCGAGGAATACACGGTCACCACC 637 439 1162 Antisense
Sequence Source Affymetrix Proprietary Database