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[193220_s_at]

Description Y49E10.3 /REP_DB=WormBase Gene ID /WP=CE22221 /GEN=pph-4.2 /TR=Q9XTT8 /GB=CAB11559.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ser-Thr protein phosphatase
Cluster 6grp_total > 88      4grp_total > 56      6grp_cgc > 59      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 86.2
(-3)
A 		114.7
(-0.8)
A 		162.7
(3)
A 		87
(-2.9)
A 		77
B 119.4
(3)
A 		68.8
(-3)
A 		100.2
(0.7)
A 		89.3
(-0.6)
A 		51
C 85.2
(-1.8)
A 		114.3
(2.9)
A 		115
(3)
A 		78
(-3)
A 		37
avr 96.9
(-1.2) 		99.3
(-0.9) 		126
(3) 		84.8
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 409
1 2 3 4 5 6 range
A 61.7
(-3)
A 		116.9
(-2)
A 		90.1
(-2.5)
A 		102
(-2.2)
P 		272.4
(1)
P 		379.8
(3)
P 		318
B 88.6
(-3)
A 		116.5
(-2.3)
A 		156.3
(-1.3)
A 		119.3
(-2.2)
M 		223.5
(0.3)
P 		330.5
(3)
P 		242
C 59.8
(-2.9)
A 		52.9
(-3)
A 		128.6
(-1.9)
A 		132.8
(-1.8)
P 		237.3
(-0.3)
P 		461.9
(3)
P 		409
avr 70
(-3) 		95.4
(-2.5) 		125
(-2) 		118
(-2.1) 		244.4
(0.3) 		390.7
(3) 		321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 31.4
(0.8)
A 		24.9
(-0.5)
A 		42.5
(3)
A 		12.7
(-3)
A 		30
B 38.1
(3)
A 		17
(-3)
A 		23.2
(-1.2)
A 		19
(-2.4)
A 		21
C 28.2
(0.3)
A 		32.9
(1.4)
A 		40.1
(3)
A 		13.2
(-3)
A 		27
avr 32.6
(2.2) 		24.9
(-0.1) 		35.3
(3) 		15
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 11.4
(-3)
A 		41.6
(-0.8)
A 		19.6
(-2.4)
A 		25.9
(-1.9)
P 		82.2
(2.2)
P 		92.5
(3)
P 		81
B 30.8
(-3)
A 		38.8
(-2.5)
A 		37.9
(-2.5)
A 		31.6
(-2.9)
M 		90.8
(0.9)
P 		123.2
(3)
P 		92
C 22.2
(-2.7)
A 		16.2
(-3)
A 		32.7
(-2.3)
A 		26.7
(-2.5)
P 		76.8
(-0.4)
P 		154.6
(3)
P 		138
avr 21.5
(-3) 		32.2
(-2.4) 		30.1
(-2.5) 		28.1
(-2.6) 		83.3
(0.6) 		123.4
(3) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193220_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6509
Sequence Type Exemplar sequence
Representative Public ID Y49E10.3 NCBI
Target Description Y49E10.3 /REP_DB=WormBase Gene ID /WP=CE22221 /GEN=pph-4.2 /TR=Q9XTT8 /GB=CAB11559.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ser-Thr protein phosphatase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027727(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027727 NCBI Caenorhabditis elegans Protein PHosphatase family member (pph-4.2) (pph-4.2) mRNA, complete cds. 11/11 None
SNAP00000016071 ENSEMBL cdna:SNAP chromosome:CEL140:III:12374361:12377123:-1 11/11 None
GENEFINDER00000016114 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12374361:12377969:-1 11/11 None
Y49E10.3a ENSEMBL cdna:known chromosome:CEL140:III:12374114:12377125:-1 gene:Y49E10.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027728 4/11 Cross Hyb Matching Probes None
Y49E10.3b 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176663 2 1 NM_001027727 193220_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12374356-12377119(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y49E10.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB11559 Hypothetical protein Y49E10.3a [Caenorhabditis elegans] ref|NP_001022898.1| Protein PHosphatase family member (pph-4.2) [Caenorhabditis elegans] 0.0
blast BAB63948.1 Ser/Thr protein phosphatase [Caenorhabditis elegans] 0.0
blast CAE66496 Hypothetical protein CBG11776 [Caenorhabditis briggsae] 1.0E-142
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 1.5E-41
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 1.5E-41
Sequence
Target Sequence 
>C. ELEGANS:193220_S_AT
accgaatcactcttatccgcggaaatcatgaaagccggcagatcacccaggtctacggtt
tctacgatgagtgtgtgagaaaatatggaagtggtcaggtctggaagcactgcaccgaga
ttttcgactacctatcgcttgcggcggttatcgatggaaagctgttctgtgtgcacggag
gattgtcaccttccatcgcaactctcgaccagatccgagttctcgatcggaagattgagg
ttccacacgagggcccgatgtgtgatctgctgtggtctgatcctgaagaggggtgctccg
gatgggggataagcccacgtggagccggttatctgttcggaggagatgctgccgagctgt
tctgcgagaataatgatttccttcgaatctgtcgagctcatcagctcgttatggaaggat
ataaacttcattttcgcaagcgggtcgtcacagtttggtcggcgcccaattactgctatc
gatgcggaaacgtggcggcaatcatggaggtcaccgaggaaaatatcgacagcgaccccg
ttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCGAATCACTCTTATCCGCGGAAA 289 89 359 Antisense
CCCAGGTCTACGGTTTCTACGATGA 149 259 404 Antisense
GGAAGCACTGCACCGAGATTTTCGA 254 531 461 Antisense
AGATTTTCGACTACCTATCGCTTGC 592 63 476 Antisense
TGCACGGAGGATTGTCACCTTCCAT 132 577 530 Antisense
GCAACTCTCGACCAGATCCGAGTTC 249 319 556 Antisense
ATCTGCTGTGGTCTGATCCTGAAGA 32 35 623 Antisense
GTGGAGCCGGTTATCTGTTCGGAGG 203 485 677 Antisense
GATTTCCTTCGAATCTGTCGAGCTC 474 427 733 Antisense
AACGTGGCGGCAATCATGGAGGTCA 330 137 847 Antisense
GAAAATATCGACAGCGACCCCGTTT 493 353 877 Antisense
Sequence Source Affymetrix Proprietary Database