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[193217_at] M-phase inducer phosphatase

Description R05H5.2 /REP_DB=WormBase Gene ID /WP=CE02289 /GEN=cdc-25.4 /TR=Q21762 /GB=CAA88725.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=M-phase inducer phosphatase
Cluster 6grp_total > 88      4grp_total > 49      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 83.1
(-2)
A 		76
(-3)
A 		119.5
(3)
A 		119.4
(3)
A 		44
B 76.9
(0.7)
A 		102.6
(3)
A 		87.8
(1.7)
A 		36.1
(-3)
A 		67
C 18.4
(-3)
A 		134.9
(3)
A 		88.5
(0.6)
A 		30.9
(-2.4)
A 		117
avr 59.5
(-3) 		104.5
(3) 		98.6
(2.2) 		62.1
(-2.7) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 72.1
(-1)
A 		37.7
(-3)
A 		86.2
(-0.2)
A 		107.7
(1)
A 		99.1
(0.5)
A 		143
(3)
A 		105
B 53.7
(-0.9)
A 		42.2
(-1.5)
A 		56.2
(-0.8)
A 		9.6
(-3)
A 		137.2
(3)
A 		137.2
(3)
P 		128
C 66.7
(-0.6)
A 		90.9
(0.5)
A 		15.8
(-3)
A 		68.8
(-0.5)
A 		98.1
(0.8)
A 		144.4
(3)
M 		129
avr 64.2
(-2.2) 		56.9
(-2.7) 		52.7
(-3) 		62
(-2.4) 		111.5
(1) 		141.5
(3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 30.3
(2.6)
A 		16.5
(-3)
A 		31.2
(3)
A 		17.5
(-2.6)
A 		15
B 24.5
(2.7)
A 		25.3
(3)
A 		20.3
(1.3)
A 		7.7
(-3)
A 		18
C 6.1
(-2.8)
A 		38.8
(3)
A 		30.8
(1.6)
A 		5.2
(-3)
A 		34
avr 20.3
(0.5) 		26.9
(2.8) 		27.4
(3) 		10.1
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 13.3
(-3)
A 		13.4
(-3)
A 		18.8
(-1.5)
A 		27.3
(0.9)
A 		29.9
(1.6)
A 		34.8
(3)
A 		22
B 18.7
(-1.2)
A 		14.1
(-1.7)
A 		13.7
(-1.7)
A 		2.6
(-3)
A 		55.8
(3)
A 		51.2
(2.5)
P 		53
C 24.7
(-0.2)
A 		27.7
(0.2)
A 		4
(-3)
A 		13.8
(-1.7)
A 		31.8
(0.8)
A 		48.3
(3)
M 		44
avr 18.9
(-1.8) 		18.4
(-1.9) 		12.2
(-3) 		14.6
(-2.6) 		39.2
(2) 		44.8
(3) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193217_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3866
Sequence Type Exemplar sequence
Representative Public ID R05H5.2 NCBI
Target Description R05H5.2 /REP_DB=WormBase Gene ID /WP=CE02289 /GEN=cdc-25.4 /TR=Q21762 /GB=CAA88725.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=M-phase inducer phosphatase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063796(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063796 NCBI Caenorhabditis elegans Cell Division Cycle related family member (cdc-25.4) (cdc-25.4) mRNA, complete cds. 11/11 None
SNAP00000021965 ENSEMBL cdna:SNAP chromosome:CEL140:II:10188221:10189958:-1 11/11 None
GENEFINDER00000021974 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10188221:10190859:-1 11/11 None
R05H5.2 ENSEMBL cdna:known chromosome:CEL140:II:10188221:10190859:-1 gene:R05H5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10188217-10190856(-) 100.0 100.0
Public Domain and Genome References
Gene Title M-phase inducer phosphatase
Gene Symbol cdc-25.4
Ensembl R05H5.2
Entrez Gene 191612 Entrez gene
SwissProt Q21762 EMBL-EBI
WormBase CE02289 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
87 M phase of mitotic cell cycle inferred from electronic annotation QuickGO AmiGO
6470 protein amino acid dephosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4725 protein tyrosine phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88725 Hypothetical protein R05H5.2 [Caenorhabditis elegans] ref|NP_496197.1| Cell Division Cycle related family member (cdc-25.4) [Caenorhabditis elegans] 0.0
blast CAA88725 Hypothetical protein R05H5.2 [Caenorhabditis elegans] ref|NP_496197.1| Cell Division Cycle related family member (cdc-25.4) [Caenorhabditis elegans] 1.0E-128
blast CAE57853 Hypothetical protein CBG00890 [Caenorhabditis briggsae] 9.0E-91
blast CAE57853 Hypothetical protein CBG00890 [Caenorhabditis briggsae] 3.0E-84
blast AAK29711 Cell division cycle related protein 25.1 [Caenorhabditis elegans] ref|NP_491862.1| Cell Division Cycle related family member (cdc-25.1) [Caenorhabditis elegans] sp|O44552|MPIP1_CAEEL M-phase inducer phosphatase cdc-25.1 (Cell division cycle related protein 25.1) 2.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR001763 EMBL-EBI Rhodanese-like 4.1E-14
Pfam IPR001763 EMBL-EBI Rhodanese-like 4.1E-14
Sequence
Target Sequence 
>C. ELEGANS:193217_AT
ttctcgttgccttcaactcattatttttgattgccggtatccatttgaatatttcggtgg
acatattaagggtgctgtaaatatatactccttggatgagttgggaaaatacctatacga
cgaatacggagtcaaaagtacattgggtggacttataccaatattttattgtgaatacag
tcaagttcgaggtccagcgatggctcgacgtcttcgaaaaatcgatacacatcgtaacaa
tcatagagctgcggcgttggatttcccagaaatctacttgcttgacaagggatatgtcaa
tttttggtcggatgtctctttaagagatctatgtgagccccgttactacatctcaatgca
tgctcgaccgtttcgacatgctctccaacagtacacacagcatcgatcgaagagcacggc
taatgatacaaagcacaagaagactcacatgagaatatttagctcggaacatcttctaga
cgaacccaaaatgatcgatgtgccgtctggatatgaaagaccaaccggagccgaagagac
gtcaactccaagacaaccaagagcccgtact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTCGTTGCCTTCAACTCATTATT 137 693 513 Antisense
CAAGTTCGAGGTCCAGCGATGGCTC 99 185 694 Antisense
ATAGAGCTGCGGCGTTGGATTTCCC 614 1 755 Antisense
GTCAATTTTTGGTCGGATGTCTCTT 393 463 808 Antisense
TAAGAGATCTATGTGAGCCCCGTTA 52 575 833 Antisense
ACTACATCTCAATGCATGCTCGACC 635 99 857 Antisense
GCATGCTCGACCGTTTCGACATGCT 676 309 870 Antisense
CATGCTCTCCAACAGTACACACAGC 379 211 889 Antisense
GGAACATCTTCTAGACGAACCCAAA 514 529 978 Antisense
ATGATCGATGTGCCGTCTGGATATG 272 45 1003 Antisense
TCCAAGACAACCAAGAGCCCGTACT 161 253 1059 Antisense
Sequence Source Affymetrix Proprietary Database