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[193210_s_at]

Description C38C3.5B /REP_DB=WormBase Gene ID /WP=CE20548 /GEN=unc-60 /TR=Q9UAU4 /GB=AAD14704.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=actin depolymerizing factor
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 24      6grp_total > 41      4grp_total > 50      6grp_cgc > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3549
1 2 3 4 range
A 1601.4
(-2.7)
P 		4600.5
(3)
P 		3538
(1)
P 		1417.5
(-3)
P 		3183
B 1761.7
(-2.1)
P 		4336.7
(3)
P 		4118.2
(2.6)
P 		1314.1
(-3)
P 		3023
C 2056
(-1.3)
P 		4552.1
(3)
P 		3459.9
(1.1)
P 		1051.9
(-3)
P 		3500
avr 1806.4
(-2) 		4496.4
(3) 		3705.4
(1.5) 		1261.2
(-3) 		3235

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3513
1 2 3 4 5 6 range
A 4303.3
(3)
P 		2827.4
(-0.1)
P 		4232
(2.8)
P 		2662.1
(-0.5)
P 		3552.5
(1.4)
P 		1467.8
(-3)
P 		2836
B 3577
(0.2)
P 		4151.4
(1.4)
P 		4980.8
(3)
P 		4635.6
(2.3)
P 		3037.8
(-0.9)
P 		1958.4
(-3)
P 		3022
C 2826.8
(-0.7)
P 		4544
(3)
P 		2856.5
(-0.6)
P 		4385.1
(2.7)
P 		2508.1
(-1.4)
P 		1766.7
(-3)
P 		2777
avr 3569
(1.8) 		3840.9
(2.5) 		4023.1
(3) 		3894.3
(2.7) 		3032.8
(0.4) 		1731
(-3) 		2292
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1131
1 2 3 4 range
A 583.1
(-0.2)
P 		998.6
(3)
P 		924.9
(2.4)
P 		207.5
(-3)
P 		791
B 561.8
(-0.9)
P 		1071.1
(3)
P 		954.3
(2.1)
P 		280
(-3)
P 		791
C 680.6
(-0.3)
P 		1308.8
(3)
P 		1205.9
(2.5)
P 		177.4
(-3)
P 		1131
avr 608.5
(-0.4) 		1126.2
(3) 		1028.4
(2.4) 		221.6
(-3) 		905

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1029
1 2 3 4 5 6 range
A 793.3
(0.7)
P 		1005.6
(2.4)
P 		921.6
(1.7)
P 		675.2
(-0.3)
P 		1072
(3)
P 		357.4
(-3)
P 		715
B 1244.6
(1.7)
P 		1381.8
(3)
P 		1209.5
(1.4)
P 		1227.8
(1.6)
P 		1234.2
(1.6)
P 		730.3
(-3)
P 		652
C 1047.8
(0.4)
P 		1386.5
(3)
P 		727
(-2)
P 		881.9
(-0.8)
P 		811.6
(-1.3)
P 		591.4
(-3)
P 		795
avr 1028.6
(1) 		1258
(3) 		952.7
(0.4) 		928.3
(0.2) 		1039.3
(1.1) 		559.7
(-3) 		698
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193210_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14916
Sequence Type Exemplar sequence
Representative Public ID C38C3.5B NCBI
Target Description C38C3.5B /REP_DB=WormBase Gene ID /WP=CE20548 /GEN=unc-60 /TR=Q9UAU4 /GB=AAD14704.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=actin depolymerizing factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071026(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071026 NCBI Caenorhabditis elegans UNCoordinated family member (unc-60) (unc-60) mRNA, complete cds. 11/11 None
SNAP00000040088 ENSEMBL cdna:SNAP chromosome:CEL140:V:1476278:1479200:1 11/11 None
GENEFINDER00000040099 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1476114:1479200:1 11/11 None
C38C3.5c.2 ENSEMBL cdna:known chromosome:CEL140:V:1474453:1479374:1 gene:C38C3.5 11/11 None
C38C3.5c.3 ENSEMBL cdna:known chromosome:CEL140:V:1474913:1479374:1 gene:C38C3.5 11/11 None
C38C3.5c.1 ENSEMBL cdna:known chromosome:CEL140:V:1474915:1479394:1 gene:C38C3.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178640 3 1 NM_071024 171748_x_at (11), 174141_at (11), 192015_s_at (11)
NM_071025 192015_s_at (11)
NM_071026 193210_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1475139-1479200(+) 99.55 99.77
Public Domain and Genome References
Ensembl C38C3.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T33952 actin depolymerizing factor homolog unc-60 - Caenorhabditis elegans 2.0E-81
blast AAL02463 Uncoordinated protein 60, isoform c [Caenorhabditis elegans] ref|NP_503427.2| UNCoordinated family member (unc-60) [Caenorhabditis elegans] sp|Q07749|ADF2_CAEEL Actin-depolymerizing factor 2 (Uncoordinated protein 60) gb|AAC14457.1| This CDS encodes the second transcript produced from the unc-60 locus. Both transcripts exhibit cofilin/destrin homologies, and share only the 5'-most exon which encodes the initiator methionine. putative [Caenorhabditis elegans] 5.0E-81
blast T33952 actin depolymerizing factor homolog unc-60 - Caenorhabditis elegans 2.0E-80
blast AAL02463 Uncoordinated protein 60, isoform c [Caenorhabditis elegans] ref|NP_503427.2| UNCoordinated family member (unc-60) [Caenorhabditis elegans] sp|Q07749|ADF2_CAEEL Actin-depolymerizing factor 2 (Uncoordinated protein 60) gb|AAC14457.1| This CDS encodes the second transcript produced from the unc-60 locus. Both transcripts exhibit cofilin/destrin homologies, and share only the 5'-most exon which encodes the initiator methionine. putative [Caenorhabditis elegans] 4.0E-80
Protein Domains
Database ID Description E-Value
Pfam IPR002108 EMBL-EBI Actin-binding, cofilin/tropomyosin type 8.8E-65
Pfam IPR002108 EMBL-EBI Actin-binding, cofilin/tropomyosin type 8.8E-65
Pfam IPR002108 EMBL-EBI Actin-binding, cofilin/tropomyosin type 8.8E-65
Sequence
Target Sequence 
>C. ELEGANS:193210_S_AT
aagttgatccatcctgcaagaacgcctacgatcttctccacaacaagcatcagcacagct
acatcatcttcaagattgacaaaaatgacaccgccatcgtcgtcgagaaggtcggagaga
agaatgctccgtacgccgagttcgttgaggagatgaagaagcttgttgaggacggaaagg
agtgcagatatgctgccgtcgacgtcgaggttactgtccaaagacagggagccgagggaa
ccagcacactcaataaggtcatcttcgttcaatactgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTTGATCCATCCTGCAAGAACGC 218 157 452 Antisense
GATCTTCTCCACAACAAGCATCAGC 649 419 481 Antisense
AGCATCAGCACAGCTACATCATCTT 590 77 497 Antisense
GACAAAAATGACACCGCCATCGTCG 303 365 529 Antisense
CCGCCATCGTCGTCGAGAAGGTCGG 82 263 542 Antisense
GAGAGAAGAATGCTCCGTACGCCGA 346 393 566 Antisense
CTCCGTACGCCGAGTTCGTTGAGGA 88 1 578 Antisense
GGAGTGCAGATATGCTGCCGTCGAC 367 517 630 Antisense
CGTCGACGTCGAGGTTACTGTCCAA 594 239 648 Antisense
AGACAGGGAGCCGAGGGAACCAGCA 294 71 673 Antisense
AAGGTCATCTTCGTTCAATACTGCC 115 161 706 Antisense
Sequence Source Affymetrix Proprietary Database