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[193209_s_at]

Description F25B5.6 /REP_DB=WormBase Gene ID /WP=CE01923 /TR=SW:Q09509 /GB=AAC46527.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=tetrahydrofolylpolyglutamate synthase
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 76      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 88      6grp_total > 67      4grp_total > 03      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2261
1 2 3 4 range
A 1481
(-1.9)
P 		1145.3
(-3)
P 		1189.9
(-2.9)
P 		2988.1
(3)
P 		1843
B 1494.9
(-2.1)
P 		1216.3
(-3)
P 		1201.4
(-3)
P 		3224.8
(3)
P 		2023
C 1410.6
(-2.2)
P 		1124.4
(-3)
P 		1199
(-2.8)
P 		3385.5
(3)
P 		2261
avr 1462.2
(-2.1) 		1162
(-3) 		1196.8
(-2.9) 		3199.5
(3) 		2038

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1064
1 2 3 4 5 6 range
A 1283.2
(-1.1)
P 		1094.2
(-2.3)
P 		968.8
(-3)
P 		1138.1
(-2)
P 		1156.9
(-1.9)
P 		1983.8
(3)
P 		1015
B 919.4
(-3)
P 		1042
(-2.1)
P 		1160.8
(-1.3)
P 		1235.9
(-0.8)
P 		1160.9
(-1.3)
P 		1775
(3)
P 		856
C 995.4
(-3)
P 		1159.3
(-1.8)
P 		1252
(-1.1)
P 		1162.8
(-1.8)
P 		1052.3
(-2.6)
P 		1815.7
(3)
P 		820
avr 1066
(-3) 		1098.5
(-2.8) 		1127.2
(-2.5) 		1178.9
(-2.1) 		1123.4
(-2.6) 		1858.2
(3) 		792
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 439
1 2 3 4 range
A 539.2
(3)
P 		248.6
(-3)
P 		311.1
(-1.7)
P 		437.4
(0.9)
P 		291
B 476.7
(-0.1)
P 		300.4
(-2.7)
P 		278.4
(-3)
P 		687.2
(3)
P 		409
C 467
(0.5)
P 		323.3
(-3)
P 		417.9
(-0.7)
P 		570.8
(3)
P 		248
avr 494.3
(1.5) 		290.8
(-3) 		335.8
(-2) 		565.1
(3) 		274

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 451
1 2 3 4 5 6 range
A 236.5
(-2.4)
P 		389.2
(0.9)
P 		211
(-3)
P 		288.7
(-1.3)
P 		349.1
(0)
P 		483
(3)
P 		272
B 319.9
(-2.4)
P 		346.8
(-2)
P 		281.9
(-3)
P 		327.3
(-2.3)
P 		471.7
(-0)
P 		661.9
(3)
P 		380
C 369
(-0.8)
P 		353.7
(-1.1)
P 		318.6
(-1.6)
P 		233.8
(-3)
P 		340.5
(-1.3)
P 		607.8
(3)
P 		374
avr 308.5
(-2.3) 		363.2
(-1.2) 		270.5
(-3) 		283.3
(-2.8) 		387.1
(-0.8) 		584.2
(3) 		314
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193209_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6634
Sequence Type Exemplar sequence
Representative Public ID F25B5.6 NCBI
Target Description F25B5.6 /REP_DB=WormBase Gene ID /WP=CE01923 /TR=SW:Q09509 /GB=AAC46527.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=tetrahydrofolylpolyglutamate synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065891(11), NM_001027377(11), NM_065890(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065891 NCBI Caenorhabditis elegans F25B5.6a (F25B5.6) mRNA, complete cds. 11/11 None
NM_001027377 NCBI Caenorhabditis elegans F25B5.6c (F25B5.6) mRNA, complete cds. 11/11 None
NM_065890 NCBI Caenorhabditis elegans F25B5.6b (F25B5.6) mRNA, complete cds. 11/11 None
SNAP00000007784 ENSEMBL cdna:SNAP chromosome:CEL140:III:5946326:5948119:-1 11/11 None
GENEFINDER00000007794 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5946326:5948119:-1 11/11 None
F25B5.6a ENSEMBL cdna:known chromosome:CEL140:III:5946174:5948591:-1 gene:F25B5.6 11/11 None
F25B5.6b ENSEMBL cdna:known chromosome:CEL140:III:5946326:5948532:-1 gene:F25B5.6 11/11 None
F25B5.6c.2 ENSEMBL cdna:known chromosome:CEL140:III:5946326:5948090:-1 gene:F25B5.6 11/11 None
F25B5.6c.1 ENSEMBL cdna:known chromosome:CEL140:III:5946326:5948658:-1 gene:F25B5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5946324-5948115(-) 100.0 100.0
Public Domain and Genome References
Ensembl F25B5.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498292 F25B5.6a [Caenorhabditis elegans] gb|AAC46527.1| Hypothetical protein F25B5.6a [Caenorhabditis elegans] sp|Q09509|FOLC_CAEEL Putative folylpolyglutamate synthase (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) 0.0
blast NP_498291 F25B5.6b [Caenorhabditis elegans] gb|AAL02494.1| Hypothetical protein F25B5.6b [Caenorhabditis elegans] 0.0
blast NP_001022548 F25B5.6c [Caenorhabditis elegans] gb|AAV58869.1| Hypothetical protein F25B5.6c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004101 EMBL-EBI Cytoplasmic peptidoglycan synthetases, C-terminal 2.6E-8
Pfam IPR004101 EMBL-EBI Cytoplasmic peptidoglycan synthetases, C-terminal 2.6E-8
Pfam IPR004101 EMBL-EBI Cytoplasmic peptidoglycan synthetases, C-terminal 2.6E-8
Pfam IPR004101 EMBL-EBI Cytoplasmic peptidoglycan synthetases, C-terminal 2.6E-8
Pfam IPR004101 EMBL-EBI Cytoplasmic peptidoglycan synthetases, C-terminal 2.6E-8
Sequence
Target Sequence 
>C. ELEGANS:193209_S_AT
gtgatgccatagaatcttgccgctggcctggaagaagtcaaatcgtttctacagatcgta
atgtcacgtatcttttggacggagcacatactccaaaaagcatggaagcctgctccgagt
gggcagccgaggaaattgtgaatttgaaaaaggagaatgtgaagaagatcctcctgtttc
aatgtactgctgacagatgcccatctactttgattaaatatttgaagcctctcggaatct
cccaaatcgtttcatgtccaactcaactccactcgagcattgataaatctgctgattcgg
ccaacctgaacgcttctcgagatgaacaagcagagaaggcaaatcaatgtgtgcaggcat
ggaaagaatctctggatcaaccggaatctgttactgaagatcaaatgaaagtatttgact
gcatatcaagtgcgtataagttcatcgaatcccaagcagcatctcaggaaatcctcgtcc
ttgtcactggaagtcttcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGATGCCATAGAATCTTGCCGCTG 63 485 1004 Antisense
AAAGCATGGAAGCCTGCTCCGAGTG 668 119 1100 Antisense
AGATCCTCCTGTTTCAATGTACTGC 181 67 1169 Antisense
TGTACTGCTGACAGATGCCCATCTA 308 559 1186 Antisense
ATATTTGAAGCCTCTCGGAATCTCC 490 19 1221 Antisense
TCTCCCAAATCGTTTCATGTCCAAC 439 611 1241 Antisense
AACTCAACTCCACTCGAGCATTGAT 51 141 1263 Antisense
CAACCTGAACGCTTCTCGAGATGAA 705 185 1305 Antisense
CATCGAATCCCAAGCAGCATCTCAG 642 211 1446 Antisense
ATCTCAGGAAATCCTCGTCCTTGTC 629 23 1464 Antisense
GTCCTTGTCACTGGAAGTCTTCATC 437 477 1480 Antisense
Sequence Source Affymetrix Proprietary Database