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[193208_s_at]

Description ZC101.2B /REP_DB=WormBase Gene ID /WP=CE15030 /GEN=unc-52 /TR=O18261 /GB=CAB07704.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=UNC-52 membrane proteoglycan
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 24      4grp_high_8_8 > 44      6grp_highest_nonorm > 40      6grp_total > 15      4grp_total > 52      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1568
1 2 3 4 range
A 1519.3
(1.8)
P 		1785
(3)
P 		1291.2
(0.7)
P 		486.7
(-3)
P 		1298
B 1469.2
(1.3)
P 		1868.6
(3)
P 		1566.4
(1.7)
P 		421.5
(-3)
P 		1447
C 1387.9
(0.7)
P 		1981
(3)
P 		1372.3
(0.7)
P 		413
(-3)
P 		1568
avr 1458.8
(1.2) 		1878.2
(3) 		1410
(1) 		440.4
(-3) 		1438

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1250
1 2 3 4 5 6 range
A 1010.2
(0.5)
P 		1482.7
(3)
P 		455
(-2.5)
P 		616.1
(-1.6)
P 		395.1
(-2.8)
P 		353.1
(-3)
P 		1130
B 1231.1
(3)
P 		870.9
(0.7)
P 		499.2
(-1.8)
P 		342.7
(-2.8)
P 		385.3
(-2.5)
P 		310.9
(-3)
P 		920
C 1301.2
(3)
P 		880.1
(0.6)
P 		847.2
(0.5)
P 		301
(-2.6)
P 		556.5
(-1.2)
P 		232.4
(-3)
P 		1069
avr 1180.8
(3) 		1077.9
(2.3) 		600.5
(-0.9) 		419.9
(-2.2) 		445.6
(-2) 		298.8
(-3) 		882
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 500
1 2 3 4 range
A 553.2
(3)
P 		387.5
(0.9)
P 		337.5
(0.3)
P 		71.2
(-3)
P 		482
B 468.5
(3)
P 		461.5
(2.9)
P 		363
(1.3)
P 		89.8
(-3)
P 		379
C 459.4
(1.7)
P 		569.6
(3)
P 		478.3
(1.9)
P 		69.6
(-3)
P 		500
avr 493.7
(3) 		472.9
(2.7) 		392.9
(1.5) 		76.9
(-3) 		417

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 467
1 2 3 4 5 6 range
A 186.2
(-1.6)
P 		527.3
(3)
P 		99.1
(-2.8)
P 		156.3
(-2)
P 		119.2
(-2.5)
P 		86
(-3)
P 		441
B 428.4
(3)
P 		289.9
(0.5)
P 		121.2
(-2.5)
P 		90.8
(-3)
P 		156.5
(-1.8)
P 		116
(-2.6)
P 		338
C 482.3
(3)
P 		268.6
(-0)
P 		215.6
(-0.8)
P 		60.5
(-3)
P 		180.1
(-1.3)
P 		77.8
(-2.8)
P 		422
avr 365.6
(3) 		361.9
(2.9) 		145.3
(-1.9) 		102.5
(-2.8) 		151.9
(-1.7) 		93.3
(-3) 		272
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193208_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3658
Sequence Type Exemplar sequence
Representative Public ID ZC101.2B NCBI
Target Description ZC101.2B /REP_DB=WormBase Gene ID /WP=CE15030 /GEN=unc-52 /TR=O18261 /GB=CAB07704.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=UNC-52 membrane proteoglycan
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064643(11), NM_001027317(11), NM_064644(11), NM_064645(11), NM_064646(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064643 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 None
NM_001027317 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 None
NM_064644 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 A 
NM_064645 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 A 
NM_064646 NCBI Caenorhabditis elegans UNCoordinated family member (unc-52) (unc-52) mRNA, complete cds. 11/11 None
SNAP00000013250 ENSEMBL cdna:SNAP chromosome:CEL140:II:14661537:14666743:-1 11/11 None
GENEFINDER00000013270 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14654105:14678428:-1 11/11 None
ZC101.2e ENSEMBL cdna:known chromosome:CEL140:II:14647215:14666743:-1 gene:ZC101.2 11/11 A 
ZC101.2f ENSEMBL cdna:known chromosome:CEL140:II:14652052:14666743:-1 gene:ZC101.2 11/11 A 
ZC101.2a ENSEMBL cdna:known chromosome:CEL140:II:14652449:14666743:-1 gene:ZC101.2 11/11 A 
ZC101.2c ENSEMBL cdna:known chromosome:CEL140:II:14652449:14666743:-1 gene:ZC101.2 11/11 A 
ZC101.2b ENSEMBL cdna:known chromosome:CEL140:II:14661162:14666834:-1 gene:ZC101.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14661533-14666740(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC101.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07708 Hypothetical protein ZC101.2e [Caenorhabditis elegans] emb|CAB07569.1| Hypothetical protein ZC101.2e [Caenorhabditis elegans] ref|NP_497044.3| UNCoordinated family member (unc-52) [Caenorhabditis elegans] sp|Q06561|UNC52_CAEEL Basement membrane proteoglycan precursor (Perlecan homolog) (Uncoordinated protein 52) pir||T19821 hypothetical protein ZC101.2e - Caenorhabditis elegans 0.0
blast CAB07706 Hypothetical protein ZC101.2a [Caenorhabditis elegans] emb|CAB07567.1| Hypothetical protein ZC101.2a [Caenorhabditis elegans] ref|NP_497046.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] 0.0
blast AAA28156 basement membrane proteoglycan 0.0
blast CAH04744 Hypothetical protein ZC101.2f [Caenorhabditis elegans] emb|CAH04704.1| Hypothetical protein ZC101.2f [Caenorhabditis elegans] ref|NP_001022488.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] 0.0
blast CAB07707 Hypothetical protein ZC101.2c [Caenorhabditis elegans] emb|CAB07568.1| Hypothetical protein ZC101.2c [Caenorhabditis elegans] ref|NP_497045.1| UNCoordinated family member (unc-52) [Caenorhabditis elegans] pir||C88369 protein unc-52 [imported] - Caenorhabditis elegans 0.0
blast CAE65897 Hypothetical protein CBG11064 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-18
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.1E-20
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 8.1E-20
Pfam IPR000034 EMBL-EBI Laminin B 9.3E-79
Pfam IPR000034 EMBL-EBI Laminin B 5.3E-73
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.7E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 7.8E-20
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 1.9E-15
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 1.1E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.4E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 1.1E-14
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-18
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.1E-20
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 8.1E-20
Pfam IPR000034 EMBL-EBI Laminin B 9.3E-79
Pfam IPR000034 EMBL-EBI Laminin B 5.3E-73
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 7.8E-20
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.7E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.41
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0010
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.26
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0014
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 1.2E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 1.4E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 7.6E-4
Pfam IPR001791 EMBL-EBI Laminin G 5.0E-5
Pfam IPR001791 EMBL-EBI Laminin G 5.3E-33
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0023
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0010
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.26
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.6E-8
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.6E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0010
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.5E-5
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 1.2E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-6
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.26
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0061
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 2.5E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.0038
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 0.13
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.8E-4
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 6.3E-9
Sequence
Target Sequence 
>C. ELEGANS:193208_S_AT
gcaacggacacgctagccaatgcgacaaggagtacggttattgcttggattgtcaacaca
acaccgaaggagatcagtgcgaaagatgcaagccaggatttgttggtgatgctcgtcgtg
gaactccaaatgactgccaaccagaagccacacgcgccccatgccattgcaacaatcact
ccccaagaggttgtgattcgttcggaagatgcttgctctgtgagcacaacaccgaaggaa
cgcattgcgagagatgcaagaagggctactacggagatgctaccaagggatccccatacg
attgtactccatgcccatgcccaggagcctccgactgctacctcgataacgaaggacaag
tggcgtgccgcatctgtccagccggactccagggacgcttgtgcaacgagtgtgctccag
gatacacccgctcaaacaagccagccggacgcgtgtgcgaaccaatcggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACGGACACGCTAGCCAATGCGA 35 321 2882 Antisense
TTTGTTGGTGATGCTCGTCGTGGAA 294 667 2980 Antisense
AATGACTGCCAACCAGAAGCCACAC 494 165 3010 Antisense
CCATGCCATTGCAACAATCACTCCC 639 271 3040 Antisense
GAAGGGCTACTACGGAGATGCTACC 612 335 3141 Antisense
CAAGGGATCCCCATACGATTGTACT 565 183 3165 Antisense
TCCGACTGCTACCTCGATAACGAAG 612 593 3211 Antisense
GAAGGACAAGTGGCGTGCCGCATCT 52 333 3232 Antisense
CAGGGACGCTTGTGCAACGAGTGTG 158 209 3271 Antisense
ACGAGTGTGCTCCAGGATACACCCG 642 93 3287 Antisense
GGACGCGTGTGCGAACCAATCGGAC 306 521 3328 Antisense
Sequence Source Affymetrix Proprietary Database