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[193205_s_at] translation initiation factor

Description C15F1.4 /REP_DB=WormBase Gene ID /WP=CE23548 /GEN=ppp-1 /TR=Q9N5Y4 /GB=AAF39757.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=translation initiation factor
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 72      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 64      6grp_total > 72      4grp_total > 60      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 592
1 2 3 4 range
A 762.4
(2.3)
P 		737.9
(2)
P 		826
(3)
P 		305.2
(-3)
P 		521
B 717.5
(1.9)
P 		649.5
(1.1)
P 		813.7
(3)
P 		285
(-3)
P 		529
C 780.3
(2.6)
P 		706.6
(1.8)
P 		821.9
(3)
P 		234
(-3)
P 		588
avr 753.4
(2.3) 		698
(1.7) 		820.5
(3) 		274.7
(-3) 		546

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 352
1 2 3 4 5 6 range
A 767.4
(-1.6)
P 		801.1
(-0.9)
P 		895.5
(1.3)
P 		878.5
(0.9)
P 		968.3
(3)
P 		708.9
(-3)
P 		259
B 805
(-2.6)
P 		787.8
(-3)
P 		949
(1.1)
P 		857.9
(-1.2)
P 		1024.5
(3)
P 		798.6
(-2.7)
P 		237
C 672.4
(-3)
P 		677.1
(-2.9)
P 		852.8
(0.1)
P 		1017.8
(3)
P 		704.2
(-2.4)
P 		752.7
(-1.6)
P 		345
avr 748.3
(-3) 		755.3
(-2.8) 		899.1
(2.3) 		918.1
(3) 		899
(2.3) 		753.4
(-2.8) 		170
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 247
1 2 3 4 range
A 277.6
(3)
P 		160.2
(-0)
P 		215.9
(1.4)
P 		44.7
(-3)
P 		233
B 228.8
(3)
P 		160.4
(0.6)
P 		188.5
(1.6)
P 		60.7
(-3)
P 		168
C 258.3
(2.3)
P 		203.2
(1)
P 		286.5
(3)
P 		39.5
(-3)
P 		247
avr 254.9
(3) 		174.6
(0.7) 		230.3
(2.3) 		48.3
(-3) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 275
1 2 3 4 5 6 range
A 141.5
(-3)
P 		284.9
(2.7)
P 		195
(-0.9)
P 		222.8
(0.2)
P 		292.2
(3)
P 		172.6
(-1.8)
P 		151
B 280.1
(-1.3)
P 		262.2
(-1.9)
P 		230.4
(-2.9)
P 		227.2
(-3)
P 		416.3
(3)
P 		297.8
(-0.8)
P 		189
C 249.2
(2.6)
P 		206.6
(-2.8)
P 		217.1
(-1.4)
P 		204.7
(-3)
P 		227.9
(-0.1)
P 		252
(3)
P 		47
avr 223.6
(-2.4) 		251.2
(-0.7) 		214.2
(-3) 		218.2
(-2.8) 		312.1
(3) 		240.8
(-1.4) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193205_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4064
Sequence Type Exemplar sequence
Representative Public ID C15F1.4 NCBI
Target Description C15F1.4 /REP_DB=WormBase Gene ID /WP=CE23548 /GEN=ppp-1 /TR=Q9N5Y4 /GB=AAF39757.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=translation initiation factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063027(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063027 NCBI Caenorhabditis elegans PyroPhosPhorylase family member (ppp-1) (ppp-1) mRNA, complete cds. 11/11 None
SNAP00000007582 ENSEMBL cdna:SNAP chromosome:CEL140:II:6961500:6967564:-1 11/11 None
GENEFINDER00000007594 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6965830:6967564:-1 11/11 None
C15F1.4 ENSEMBL cdna:known chromosome:CEL140:II:6965788:6967579:-1 gene:C15F1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6965825-6967569(-) 100.0 100.0
Public Domain and Genome References
Gene Title translation initiation factor
Gene Symbol ppp-1
Ensembl C15F1.4
Entrez Gene 174138 Entrez gene
SwissProt P80361 EMBL-EBI
WormBase CE23548 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1595996_S_AT similar to Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE:1588630_S_AT similar to Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFA.453.1.A1_AT similar to Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFAAFFX.7951.1.S1_S_AT similar to Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFAAFFX.7954.1.S1_AT similar to Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) cfa
CANINE_2:CFAAFFX.7954.1.S1_X_AT similar to Translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) cfa
DROSOPHILA_2:1638841_AT dm
DROSGENOME1:141601_AT dm
CHICKEN:GGA.5193.1.S1_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa gga
CHICKEN:GGAAFFX.13172.1.S1_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa gga
HG-U133_PLUS_2:218488_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-U133A_2:218488_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-U133A:218488_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-FOCUS:218488_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HU35KSUBA:W58049_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HU35KSUBC:RC_F01614_S_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
U133_X3P:G9966778_3P_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-U95B:54927_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-U95B:54929_G_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-U95C:64934_AT eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HG-U95E:69911_AT Eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
HU35KSUBD:RC_C21034_AT Eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa hs
MG-U74BV2:115482_AT eukaryotic translation initiation factor 2B, subunit 3 mm
MOE430B:1434524_AT eukaryotic translation initiation factor 2B, subunit 3 mm
MOUSE430_2:1434524_AT eukaryotic translation initiation factor 2B, subunit 3 mm
MG-U74CV2:139598_AT PREDICTED: eukaryotic translation initiation factor 2B, subunit 3 [Mus musculus], mRNA sequence mm
MU19KSUBA:TC14960_AT PREDICTED: eukaryotic translation initiation factor 2B, subunit 3 [Mus musculus], mRNA sequence mm
RG-U34A:RC_AI639441_AT eukaryotic translation initiation factor 2B, subunit 3 gamma rn
RG-U34A:U38253_AT eukaryotic translation initiation factor 2B, subunit 3 gamma rn
RG-U34A:U38253_G_AT eukaryotic translation initiation factor 2B, subunit 3 gamma rn
RAE230A:1368967_AT eukaryotic translation initiation factor 2B, subunit 3 gamma rn
RAT230_2:1368967_AT eukaryotic translation initiation factor 2B, subunit 3 gamma rn
Protein Similarities
Method ID Description E-Value
blast AAF39757 Pyrophosphorylase family protein 1 [Caenorhabditis elegans] ref|NP_495428.1| PyroPhosPhorylase family member (ppp-1) [Caenorhabditis elegans] sp|P80361|EI2BG_CAEEL Putative translation initiation factor eIF-2B gamma subunit (eIF-2B GDP-GTP exchange factor) (Putative pyrophosphorylase ppp-1) 0.0
blast AAF39756 Transformer : xx animals transformed into males protein 2, isoform b [Caenorhabditis elegans] ref|NP_001021954.1| TRAnsformer : XX animals transformed into males family member (tra-2) [Caenorhabditis elegans] 0.0
blast AAF39755 Transformer : xx animals transformed into males protein 2, isoform a [Caenorhabditis elegans] ref|NP_495426.1| TRAnsformer : XX animals transformed into males family member (tra-2) [Caenorhabditis elegans] gb|AAB22845.1| pTra2A [Caenorhabditis elegans] sp|P34709|TRA2_CAEEL Sex-determining transformer protein 2 precursor (Ce-Tra-2) gb|AAA28150.1| membrane protein 0.0
blast CAE66003 Hypothetical protein CBG11194 [Caenorhabditis briggsae] 0.0
blast S71475 pyrophosphorylase ppp-1 homolog - Caenorhabditis elegans gb|AAA64269.1| putative pyrophosphorylase 1.0E-180
blast S71475 pyrophosphorylase ppp-1 homolog - Caenorhabditis elegans gb|AAA64269.1| putative pyrophosphorylase 1.0E-178
blast CAE66003 Hypothetical protein CBG11194 [Caenorhabditis briggsae] 1.0E-155
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000007582 5 834-856,863-885,895-912,972-994,1122-1144
Sequence
Target Sequence 
>C. ELEGANS:193205_S_AT
gattgtcatgtttatgctatacgtcataaagttcttcttaatctgtcaaaatcaaaacac
atttcatcattcaaagccgattttgtgcctcttctgattgataaacaatttgaaccggat
tctgatatcaaatgctttgcatatcgtcttcctcatgaaaatggatttgtaactgcacat
gcgaatactcttggctcttatttcgaagtgaataaagctattcaaaaatcttttactcga
ttgatggaatatcgcggaaatgggaagaatttcaactacaaaactgataaaattgctgcc
cacgagagtcggatcgaagaaagcgcagagattgacaaggattcagttatcaaacggtct
ttcatctcggataattgtcggattggtgaaaaaacaaagctaaaagagagtatcattgcc
aaaggcgttgtcattggaaatggtgcctccataagcaactctattatttgtgatggagta
gaaatcggagagaatgctgacgtcaccaattgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGTCATGTTTATGCTATACGTC 489 433 622 Antisense
GCTATACGTCATAAAGTTCTTCTTA 694 303 637 Antisense
TCAAAGCCGATTTTGTGCCTCTTCT 543 629 692 Antisense
TTTGTGCCTCTTCTGATTGATAAAC 9 667 703 Antisense
AAATGCTTTGCATATCGTCTTCCTC 651 119 751 Antisense
GCACATGCGAATACTCTTGGCTCTT 71 317 796 Antisense
TACTCTTGGCTCTTATTTCGAAGTG 152 645 807 Antisense
TAAAATTGCTGCCCACGAGAGTCGG 490 627 909 Antisense
CAAACGGTCTTTCATCTCGGATAAT 589 179 972 Antisense
GGTGCCTCCATAAGCAACTCTATTA 463 501 1063 Antisense
GAGAATGCTGACGTCACCAATTGCA 216 389 1111 Antisense
Sequence Source Affymetrix Proprietary Database