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[193195_at] Ser/Thr protein phosphatase

Description Y75B8A.30 /REP_DB=WormBase Gene ID /WP=CE23041 /GEN=pph-4.1 /TR=Q9XW79 /GB=CAA22090.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ser-Thr protein phosphatase
Cluster 6grp_highest_10_10 > 81      6grp_high1_10_10 > 29      4grp_highest_8_8 > 16      4grp_high_8_8 > 53      6grp_highest_nonorm > 71      6grp_total > 28      4grp_total > 59      6grp_highest_sub > Cu      6grp_cgc > 03      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 248
1 2 3 4 range
A 424.7
(0.3)
P 		522
(3)
P 		502.5
(2.5)
P 		307.6
(-3)
P 		214
B 432.4
(-0.1)
P 		494.6
(1.5)
P 		555.6
(3)
P 		317.1
(-3)
P 		239
C 455.9
(0.6)
P 		546.9
(3)
P 		473.1
(1)
P 		323.7
(-3)
P 		223
avr 437.7
(0.6) 		521.2
(3) 		510.4
(2.7) 		316.1
(-3) 		205

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 333.2
(1)
P 		366.4
(3)
P 		266.8
(-2.9)
P 		264.4
(-3)
P 		303.8
(-0.7)
P 		287
(-1.7)
P 		102
B 322.7
(0.7)
P 		299.6
(-1.8)
P 		337.8
(2.4)
P 		289.2
(-3)
P 		342.9
(3)
P 		312.6
(-0.4)
P 		54
C 405.9
(3)
P 		330.1
(-1.9)
P 		313.9
(-3)
P 		315
(-2.9)
P 		319.5
(-2.6)
P 		331.5
(-1.9)
P 		92
avr 353.9
(3) 		332
(1) 		306.2
(-1.4) 		289.5
(-3) 		322.1
(0) 		310.4
(-1.1) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 154.6
(3)
P 		113.3
(0.7)
P 		131.4
(1.7)
P 		45
(-3)
P 		110
B 137.9
(3)
P 		122.2
(1.7)
P 		128.7
(2.2)
P 		67.6
(-3)
P 		70
C 150.9
(2.2)
P 		157.2
(2.6)
P 		164.9
(3)
P 		54.6
(-3)
P 		110
avr 147.8
(3) 		130.9
(1.9) 		141.7
(2.6) 		55.7
(-3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 61.4
(-2.7)
P 		130.3
(3)
P 		58.1
(-3)
P 		67.1
(-2.3)
P 		91.7
(-0.2)
P 		69.9
(-2)
P 		72
B 112.3
(0.4)
P 		99.7
(-0.8)
P 		82
(-2.5)
P 		76.6
(-3)
P 		139.3
(3)
P 		116.6
(0.8)
P 		63
C 150.4
(3)
P 		100.7
(-0.4)
P 		79.9
(-1.9)
P 		63.3
(-3)
P 		103.4
(-0.2)
P 		111
(0.3)
P 		87
avr 108
(2.5) 		110.2
(2.8) 		73.3
(-2.4) 		69
(-3) 		111.5
(3) 		99.2
(1.3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193195_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7603
Sequence Type Exemplar sequence
Representative Public ID Y75B8A.30 NCBI
Target Description Y75B8A.30 /REP_DB=WormBase Gene ID /WP=CE23041 /GEN=pph-4.1 /TR=Q9XW79 /GB=CAA22090.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Ser-Thr protein phosphatase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067202(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067202 NCBI Caenorhabditis elegans Protein PHosphatase family member (pph-4.1) (pph-4.1) mRNA, complete cds. 11/11 None
SNAP00000020098 ENSEMBL cdna:SNAP chromosome:CEL140:III:12345440:12353643:-1 11/11 None
GENEFINDER00000020200 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12345440:12351205:-1 11/11 None
Y75B8A.30 ENSEMBL cdna:known chromosome:CEL140:III:12345440:12351213:-1 gene:Y75B8A.30 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12345435-12351201(-) 100.0 100.0
Public Domain and Genome References
Gene Title Ser/Thr protein phosphatase
Gene Symbol pph-4.1
Ensembl Y75B8A.30
Entrez Gene 176657 Entrez gene
SwissProt Q9XW79 EMBL-EBI
WormBase CE23041 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17822_S_AT serine/threonine protein phosphatase PP-X isozyme 2 (PPX2) at
ATH1-121501:248097_AT serine/threonine protein phosphatase PP-X isozyme 2 (PPX2) at
CANINE_2:CFAAFFX.26146.1.S1_AT similar to Serine/threonine protein phosphatase 4 catalytic subunit (PP4C) (Pp4) (Protein phosphatase X) (PP-X) cfa
DROSOPHILA_2:1623506_S_AT Protein phosphatase 19C dm
DROSGENOME1:143976_AT Protein phosphatase 19C dm
HG-U95AV2:382_AT protein phosphatase 4 (formerly X), catalytic subunit hs
HC-G110:382_AT protein phosphatase 4 (formerly X), catalytic subunit hs
HG-U133A_2:208932_AT protein phosphatase 4 (formerly X), catalytic subunit hs
HG-U133A:208932_AT protein phosphatase 4 (formerly X), catalytic subunit hs
HG-U133_PLUS_2:208932_AT protein phosphatase 4 (formerly X), catalytic subunit hs
HG-FOCUS:208932_AT protein phosphatase 4 (formerly X), catalytic subunit hs
HUGENEFL:X70218_AT protein phosphatase 4 (formerly X), catalytic subunit hs
U133_X3P:G12655126_3P_S_AT protein phosphatase 4 (formerly X), catalytic subunit hs
MOUSE430A_2:1460288_A_AT protein phosphatase 4, catalytic subunit mm
MOUSE430_2:1460288_A_AT protein phosphatase 4, catalytic subunit mm
MOE430A:1460288_A_AT protein phosphatase 4, catalytic subunit mm
MU11KSUBB:MSA.17599.0_S_AT protein phosphatase 4, catalytic subunit mm
MU11KSUBB:MSA.15565.0_F_AT protein phosphatase 4, catalytic subunit mm
MU11KSUBB:MSA.36629.0_S_AT protein phosphatase 4, catalytic subunit mm
MG-U74AV2:95631_AT protein phosphatase 4, catalytic subunit mm
MOUSE430_2:1445350_AT Protein phosphatase 4, catalytic subunit, mRNA (cDNA clone MGC:5728 IMAGE:3491568) mm
MOE430B:1445350_AT Protein phosphatase 4, catalytic subunit, mRNA (cDNA clone MGC:5728 IMAGE:3491568) mm
MU19KSUBB:TC30731_AT Protein phosphatase 4, catalytic subunit, mRNA (cDNA clone MGC:5728 IMAGE:3491568) mm
RG-U34B:RC_AA957050_AT protein phosphatase 4 (formerly X), catalytic subunit rn
RAE230A:1390333_AT protein phosphatase 4 (formerly X), catalytic subunit rn
RAT230_2:1390333_AT protein phosphatase 4 (formerly X), catalytic subunit rn
YEAST_2:1780005_AT Catalytic subunit of protein phosphatase; involved in activation of Gln3p, which is a transcription factor with a role in nitrogen utilization Sc
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA22090 Hypothetical protein Y75B8A.30 [Caenorhabditis elegans] ref|NP_499603.1| Protein PHosphatase family member (pph-4.1) [Caenorhabditis elegans] 0.0
blast CAE66496 Hypothetical protein CBG11776 [Caenorhabditis briggsae] 0.0
blast CAA22090 Hypothetical protein Y75B8A.30 [Caenorhabditis elegans] ref|NP_499603.1| Protein PHosphatase family member (pph-4.1) [Caenorhabditis elegans] 1.0E-179
blast BAB63947.1 Ser/Thr protein phosphatase [Caenorhabditis elegans] 1.0E-176
blast CAE66496 Hypothetical protein CBG11776 [Caenorhabditis briggsae] 1.0E-176
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 5.7E-44
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 5.7E-44
Sequence
Target Sequence 
>C. ELEGANS:193195_AT
gaaagctcgctatccggatcgaatgatgttgatccgtggaaatcacgaaagtcgccagat
tactcaagtctacgggttctatgatgaatgccttcggaaatatggaaatgcgtctgtttg
gaaacattgtaccgaagttttcgactacttatcacttgccgctgtaattgatgggaaggt
tttctgtgttcatggtggattatccccgtcgattagtactatggatcagattcgtgttat
tgatcggaagcaggaggttccccacgacggtccaatgtgtgatctactgtggtcggaccc
cgaagaaggaaatgttggatggggcctaagtccacgcggagccggttatctattcggagc
ggacgcgtcgaaaacgttttgcgaaacgaacggagtcgatttgatctgtagagctcatca
attggttatggaaggatataaatggcatttcaacgagaaagtgctcactgtttggtcggc
gcccaactattgttatcgatgcggcaacgtggcggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGCTCGCTATCCGGATCGAATG 573 359 387 Antisense
GATTACTCAAGTCTACGGGTTCTAT 73 397 444 Antisense
TTTTCGACTACTTATCACTTGCCGC 9 677 524 Antisense
GGATTATCCCCGTCGATTAGTACTA 540 507 583 Antisense
ATCGGAAGCAGGAGGTTCCCCACGA 700 33 629 Antisense
TCCCCACGACGGTCCAATGTGTGAT 69 597 645 Antisense
ATGTGTGATCTACTGTGGTCGGACC 621 49 661 Antisense
TAAGTCCACGCGGAGCCGGTTATCT 59 637 713 Antisense
GTTATCTATTCGGAGCGGACGCGTC 467 445 731 Antisense
TCGGCGCCCAACTATTGTTATCGAT 479 607 862 Antisense
GTTATCGATGCGGCAACGTGGCGGC 407 443 878 Antisense
Sequence Source Affymetrix Proprietary Database