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[193184_at] phosphatidylinositol biosynthetic protein

Description D2085.6 /REP_DB=WormBase Gene ID /WP=CE03109 /TR=Q18993 /GB=CAA91062.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=phosphatidylinositol biosynthetic protein
Cluster 6grp_highest_10_10 > 49      6grp_high1_10_10 > 94      4grp_highest_8_8 > 57      4grp_high_8_8 > 33      6grp_highest_nonorm > 81      6grp_total > 74      4grp_total > 46      6grp_highest_sub > 04_05_06      6grp_high1_sub > In      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 248.6
(3)
P 		205.1
(0)
P 		177.1
(-1.9)
P 		161.1
(-3)
P 		88
B 282.6
(3)
P 		208.6
(-0.2)
P 		143.4
(-3)
P 		151.9
(-2.6)
P 		139
C 263
(3)
P 		182.3
(-3)
P 		208
(-1.1)
P 		193.5
(-2.2)
P 		81
avr 264.7
(3) 		198.7
(-1.1) 		176.2
(-2.5) 		168.8
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 160.1
(-3)
P 		234.5
(-0.5)
P 		242.5
(-0.2)
P 		335.6
(3)
P 		310.7
(2.1)
P 		282.1
(1.2)
P 		176
B 218.2
(-3)
P 		239
(-2)
P 		289.7
(0.3)
P 		329.8
(2.1)
P 		303.5
(0.9)
P 		349.4
(3)
P 		131
C 216.8
(-3)
P 		256.6
(-0.3)
P 		284.9
(1.7)
P 		257.2
(-0.2)
P 		258.8
(-0.1)
P 		303.9
(3)
P 		87
avr 198.4
(-3) 		243.4
(-0.6) 		272.4
(0.9) 		307.5
(2.8) 		291
(1.9) 		311.8
(3) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 90.5
(3)
P 		44.5
(-1.1)
P 		46.3
(-1)
P 		23.6
(-3)
P 		67
B 90.1
(3)
P 		51.5
(-1)
P 		33.2
(-2.9)
P 		32.4
(-3)
P 		58
C 87.1
(3)
P 		52.4
(-0.8)
P 		72.5
(1.4)
P 		32.6
(-3)
P 		55
avr 89.2
(3) 		49.5
(-1) 		50.7
(-0.9) 		29.5
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 29.5
(-3)
P 		83.4
(2)
P 		52.8
(-0.8)
P 		85.1
(2.2)
P 		93.8
(3)
P 		68.7
(0.7)
P 		64
B 75.9
(-2.4)
P 		79.6
(-2.1)
P 		70.4
(-3)
P 		87.4
(-1.3)
P 		123.3
(2.3)
P 		130.3
(3)
P 		60
C 80.3
(0.4)
P 		78.3
(0.2)
P 		72.5
(-0.5)
P 		51.7
(-3)
P 		83.8
(0.9)
P 		101.7
(3)
P 		50
avr 61.9
(-3) 		80.4
(-0.1) 		65.2
(-2.5) 		74.7
(-1) 		100.3
(3) 		100.2
(3) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193184_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4006
Sequence Type Exemplar sequence
Representative Public ID D2085.6 NCBI
Target Description D2085.6 /REP_DB=WormBase Gene ID /WP=CE03109 /TR=Q18993 /GB=CAA91062.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=phosphatidylinositol biosynthetic protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063439(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063439 NCBI Caenorhabditis elegans D2085.6 (D2085.6) mRNA, complete cds. 11/11 None
SNAP00000003893 ENSEMBL cdna:SNAP chromosome:CEL140:II:8659798:8672128:-1 11/11 None
GENEFINDER00000003899 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8659798:8661511:-1 11/11 None
D2085.6 ENSEMBL cdna:known chromosome:CEL140:II:8659798:8661564:-1 gene:D2085.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8659794-8661508(-) 100.0 100.0
Public Domain and Genome References
Gene Title phosphatidylinositol biosynthetic protein
Ensembl D2085.6
Entrez Gene 174386 Entrez gene
SwissProt Q18993 EMBL-EBI
WormBase CE03109 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:252617_AT N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein, putative at
CANINE_2:CFAAFFX.18538.1.S1_AT similar to phosphatidylinositol N-acetylglucosaminyltransferase subunit A isoform 1 cfa
DROSOPHILA_2:1635762_AT dm
DROSGENOME1:155058_AT dm
CHICKEN:GGAAFFX.10597.1.S1_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) gga
HG-U95AV2:221_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HC-G110:221_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
PLASMODIUM_ANOPHELES:205281_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U133A:205281_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U133_PLUS_2:205281_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-FOCUS:205281_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U133A_2:205281_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HUGENEFL:S78467_S_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HUGENEFL:D28791_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
U133_X3P:G11863129_3P_A_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U95AV2:39993_AT phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U133_PLUS_2:215969_AT Phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U133A_2:215969_AT Phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U133A:215969_AT Phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
U133_X3P:HS.137154.0.S1_3P_AT Phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
HG-U95AV2:33031_AT Phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) hs
MOUSE430_2:1453497_A_AT phosphatidylinositol glycan, class A mm
MOE430A:1453497_A_AT phosphatidylinositol glycan, class A mm
MOUSE430A_2:1453497_A_AT phosphatidylinositol glycan, class A mm
MU11KSUBA:D31863_S_AT phosphatidylinositol glycan, class A mm
MG-U74AV2:92304_AT phosphatidylinositol glycan, class A mm
MG-U74AV2:161433_F_AT phosphatidylinositol glycan, class A mm
MOE430A:1427305_AT phosphatidylinositol glycan, class A mm
MOUSE430A_2:1427305_AT phosphatidylinositol glycan, class A mm
MOUSE430_2:1427305_AT phosphatidylinositol glycan, class A mm
MU19KSUBB:TC30199_AT Phosphatidylinositol glycan, class A (Piga), mRNA mm
RAE230B:1396561_X_AT phosphatidylinositol glycan, class A rn
RAT230_2:1396561_X_AT phosphatidylinositol glycan, class A rn
YEAST_2:1773839_AT UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91062 Hypothetical protein D2085.6 [Caenorhabditis elegans] ref|NP_495840.1| D2085.6 [Caenorhabditis elegans] 0.0
blast CAE57536 Hypothetical protein CBG00513 [Caenorhabditis briggsae] 0.0
blast CAA91265 Hypothetical protein D2085.5a [Caenorhabditis elegans] emb|CAA91065.2| Hypothetical protein D2085.5a [Caenorhabditis elegans] ref|NP_001022054.1| D2085.5a [Caenorhabditis elegans] 0.0
blast CAE17686 Hypothetical protein D2085.5b [Caenorhabditis elegans] emb|CAE17775.1| Hypothetical protein D2085.5b [Caenorhabditis elegans] ref|NP_001022055.1| D2085.5b [Caenorhabditis elegans] 0.0
blast XP_397085 PREDICTED: similar to phosphatidylinositol N-acetylglucosaminyltransferase subunit A [Apis mellifera] 1.0E-117
Protein Domains
Database ID Description E-Value
Pfam IPR001296 EMBL-EBI Glycosyl transferase, group 1 3.2E-25
Pfam IPR001296 EMBL-EBI Glycosyl transferase, group 1 3.2E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495840.1 1 397-419
D2085.6 1 397-419
SNAP00000003893 2 7-29,876-898
GENEFINDER00000003899 1 397-419
Sequence
Target Sequence 
>C. ELEGANS:193184_AT
gagtcgttattctcggaatgctcccccataatcaagtgaaacgagtcttaaatcaaggac
aaattttcataaatacatcgcttactgaagcgttttgtatgagcattgttgaagccgcga
gttgtggtttacatgtagtctctactcgagttggaggtgttcctgaagttcttccaattg
gagaattcatatcgttagaagaaccagttccagatgatctcgttgatgctcttctaaaag
cggttgatcgtcgagaaaaaggtctattgatggatcctactgaaaaacatgaagctgttt
caaaaatgtacaactggccggatgtagctgcaagaactcaagttatctatcaaaaagccg
tcgagtcggagcctacaggaagattaggaagattaaaagggtattacgatcaaggaatcg
ggtttggaatcatgtatatcgttgtttcgtgtatcataattttctggttaacagtcctcg
atttgtttgattctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCGTTATTCTCGGAATGCTCCC 350 399 791 Antisense
ATGCTCCCCCATAATCAAGTGAAAC 252 41 808 Antisense
GAAGCCGCGAGTTGTGGTTTACATG 32 345 901 Antisense
GTAGTCTCTACTCGAGTTGGAGGTG 130 457 925 Antisense
GGAGGTGTTCCTGAAGTTCTTCCAA 252 515 943 Antisense
AGAACCAGTTCCAGATGATCTCGTT 95 75 990 Antisense
GATCTCGTTGATGCTCTTCTAAAAG 118 415 1006 Antisense
ACAACTGGCCGGATGTAGCTGCAAG 323 113 1100 Antisense
AAAAGCCGTCGAGTCGGAGCCTACA 238 131 1143 Antisense
GTATATCGTTGTTTCGTGTATCATA 410 453 1224 Antisense
CAGTCCTCGATTTGTTTGATTCTCC 61 209 1262 Antisense
Sequence Source Affymetrix Proprietary Database