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[193178_at] phytochelatin synthase

Description F54D5.1 /REP_DB=WormBase Gene ID /WP=CE11062 /TR=O17885 /GB=CAA91338.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=S.pombe hypothetical protein C3H1.10 like
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 48      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 82      6grp_total > 48      4grp_total > 43      6grp_highest_sub > Cu      6grp_cgc > 07      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 400.2
(2.1)
P 		422.1
(3)
P 		280.2
(-3)
P 		345.6
(-0.2)
P 		142
B 317.1
(-3)
P 		520.7
(3)
P 		348.2
(-2.1)
P 		374.5
(-1.3)
P 		204
C 303
(-3)
P 		443.1
(3)
P 		417.2
(1.9)
P 		365.8
(-0.3)
P 		140
avr 340.1
(-3) 		462
(3) 		348.5
(-2.6) 		362
(-1.9) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 383.1
(3)
P 		288.1
(-1.9)
P 		335.6
(0.5)
P 		267.8
(-3)
P 		321.3
(-0.2)
P 		367.4
(2.2)
P 		115
B 453.9
(3)
P 		266.7
(-3)
P 		351.5
(-0.3)
P 		325.3
(-1.1)
P 		340.9
(-0.6)
P 		377.4
(0.5)
P 		187
C 358.6
(0.6)
P 		315.7
(-0.7)
P 		238.5
(-3)
P 		276.5
(-1.9)
P 		296.1
(-1.3)
P 		438.7
(3)
P 		200
avr 398.5
(3) 		290.2
(-3) 		308.5
(-2) 		289.9
(-3) 		319.4
(-1.4) 		394.5
(2.8) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 145.7
(3)
P 		91.6
(-0.4)
P 		73.3
(-1.6)
P 		50.6
(-3)
P 		95
B 101.1
(-0.4)
P 		128.6
(3)
P 		80.7
(-2.9)
P 		79.8
(-3)
P 		49
C 100.3
(-0.2)
P 		127.4
(1.7)
P 		145.4
(3)
P 		61.7
(-3)
P 		84
avr 115.7
(3) 		115.9
(3) 		99.8
(1.1) 		64
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 70.6
(-2.5)
P 		102.5
(3)
P 		73.1
(-2.1)
P 		67.9
(-3)
P 		97
(2)
P 		89.4
(0.7)
P 		35
B 158
(3)
P 		88.8
(-2.7)
P 		85.3
(-3)
P 		86.2
(-2.9)
P 		138.5
(1.4)
P 		140.7
(1.6)
P 		73
C 132.9
(2.1)
P 		96.3
(-0.3)
P 		60.7
(-2.7)
P 		55.6
(-3)
P 		95.8
(-0.4)
P 		146.9
(3)
P 		91
avr 120.5
(2.4) 		95.9
(-0.2) 		73
(-2.7) 		69.9
(-3) 		110.4
(1.4) 		125.7
(3) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193178_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3135
Sequence Type Exemplar sequence
Representative Public ID F54D5.1 NCBI
Target Description F54D5.1 /REP_DB=WormBase Gene ID /WP=CE11062 /TR=O17885 /GB=CAA91338.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=S.pombe hypothetical protein C3H1.10 like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064074(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064074 NCBI Caenorhabditis elegans PhytoChelatin Synthase family member (pcs-1) (pcs-1) mRNA, complete cds. 11/11 None
SNAP00000004676 ENSEMBL cdna:SNAP chromosome:CEL140:II:11572470:11577091:-1 11/11 None
GENEFINDER00000004688 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11574904:11577091:-1 11/11 None
F54D5.1 ENSEMBL cdna:known chromosome:CEL140:II:11575597:11577091:-1 gene:F54D5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11575593-11577088(-) 100.0 100.0
Public Domain and Genome References
Gene Title phytochelatin synthase
Gene Symbol pcs-1
Ensembl F54D5.1
Entrez Gene 174775 Entrez gene
SwissProt O17885 EMBL-EBI
WormBase CE11062 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:265065_AT phytochelatin synthase 2 (PCS2) at
ATGENOME1:12669_AT phytochelatin synthase 2 (PCS2) at
Protein Similarities
Method ID Description E-Value
blast CAA91338 Hypothetical protein F54D5.1 [Caenorhabditis elegans] ref|NP_496475.3| PhytoChelatin Synthase family member (pcs-1) [Caenorhabditis elegans] gb|AAK62992.1| phytochelatin synthase [Caenorhabditis elegans] gb|AAK62991.1| phytochelatin synthase [Caenorhabditis elegans] 0.0
blast CAE59471 Hypothetical protein CBG02855 [Caenorhabditis briggsae] 1.0E-172
blast CAA91338 Hypothetical protein F54D5.1 [Caenorhabditis elegans] ref|NP_496475.3| PhytoChelatin Synthase family member (pcs-1) [Caenorhabditis elegans] gb|AAK62992.1| phytochelatin synthase [Caenorhabditis elegans] gb|AAK62991.1| phytochelatin synthase [Caenorhabditis elegans] 1.0E-169
blast CAE59471 Hypothetical protein CBG02855 [Caenorhabditis briggsae] 1.0E-146
blast XP_635353 hypothetical protein DDB0219651 [Dictyostelium discoideum] gb|EAL61877.1| hypothetical protein DDB0219651 [Dictyostelium discoideum] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR007719 EMBL-EBI Phytochelatin synthase 1.5E-41
Pfam IPR007719 EMBL-EBI Phytochelatin synthase 1.6E-38
Pfam IPR007719 EMBL-EBI Phytochelatin synthase 1.0E-122
Sequence
Target Sequence 
>C. ELEGANS:193178_AT
gccgcctatcacgaggattctgatcaagttctgattatggacgttgcaagattcaagtat
ccacctcattgggtgaaattggagactcttcagaaggcattgtgctccgttgatgttaca
acaaaactaccacgtgggctcgtggagctcgaactaaaaaaaggaacgaggcctctgata
atgtatggattgaaagcctatgtgaacatcaatgattccgattttgccacgtcagtgatc
tcgtggaatcaattcttattatgtgatccattagaagacgacgaagaagaattccaattg
tgctgtcgaaagttcgggcaatgttttgctccccacgcaatgtgctgcactcagaaaact
ttcgacgcagatcaaaaaaactcgtgtacagaatgctcaaccgatcagaatgaggcttgc
aagatgatttgttcggaaatacgaagaacccgatttgccgaagtgttctctagttcggcg
gttgctgctcttctcatagcgtggccattcgaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGCCTATCACGAGGATTCTGATC 556 285 520 Antisense
GATTCAAGTATCCACCTCATTGGGT 142 431 569 Antisense
AAGGCATTGTGCTCCGTTGATGTTA 413 161 613 Antisense
CACGTGGGCTCGTGGAGCTCGAACT 110 197 650 Antisense
TCAATGATTCCGATTTTGCCACGTC 598 631 728 Antisense
GATTTTGCCACGTCAGTGATCTCGT 662 429 739 Antisense
CCCACGCAATGTGCTGCACTCAGAA 418 257 851 Antisense
GTACAGAATGCTCAACCGATCAGAA 646 459 905 Antisense
GAAGAACCCGATTTGCCGAAGTGTT 202 337 962 Antisense
CGAAGTGTTCTCTAGTTCGGCGGTT 123 253 978 Antisense
CTTCTCATAGCGTGGCCATTCGAAA 432 219 1009 Antisense
Sequence Source Affymetrix Proprietary Database