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[193168_at] NADH_ubiquinone oxidoreductase subunit

Description F22D6.4 /REP_DB=WormBase Gene ID /WP=CE05685 /TR=SW:Q19724 /GB=CAA95810.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH_ubiquinone oxidoreductase subunit
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 36      6grp_total > 52      4grp_total > 58      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2825
1 2 3 4 range
A 3628.1
(-0)
P 		5054.2
(3)
P 		4121.6
(1)
P 		2229
(-3)
P 		2825
B 3242.2
(-1)
P 		4651
(3)
P 		4398.9
(2.3)
P 		2532.1
(-3)
P 		2119
C 3839
(0.6)
P 		4182.4
(1.7)
P 		4624.1
(3)
P 		2647.5
(-3)
P 		1977
avr 3569.8
(0.1) 		4629.2
(3) 		4381.5
(2.3) 		2469.5
(-3) 		2160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2400
1 2 3 4 5 6 range
A 3324.8
(-3)
P 		4552.8
(0.3)
P 		5258.7
(2.1)
P 		5581.6
(3)
P 		3572.2
(-2.3)
P 		3703.4
(-2)
P 		2257
B 3766.3
(-2.3)
P 		4272.7
(-0.9)
P 		5724.4
(3)
P 		4474.7
(-0.4)
P 		3878.2
(-2)
P 		3505.2
(-3)
P 		2219
C 4494.6
(-0.2)
P 		4324.3
(-0.7)
P 		5595
(3)
P 		4326.7
(-0.7)
P 		4542.9
(-0.1)
P 		3553.1
(-3)
P 		2042
avr 3861.9
(-2.1) 		4383.3
(-0.5) 		5526
(3) 		4794.3
(0.7) 		3997.8
(-1.7) 		3587.2
(-3) 		1939
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1285
1 2 3 4 range
A 1321
(3)
P 		1097.1
(1.6)
P 		1077.5
(1.5)
P 		326.3
(-3)
P 		995
B 1033.9
(1.9)
P 		1148.7
(3)
P 		1019.3
(1.7)
P 		539.6
(-3)
P 		609
C 1270.8
(1.2)
P 		1202.5
(0.9)
P 		1611.6
(3)
P 		446.4
(-3)
P 		1165
avr 1208.6
(2.8) 		1149.4
(2.3) 		1236.1
(3) 		437.4
(-3) 		799

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1053
1 2 3 4 5 6 range
A 612.9
(-3)
P 		1619.2
(3)
P 		1145.2
(0.2)
P 		1415.6
(1.8)
P 		1077.9
(-0.2)
P 		901.7
(-1.3)
P 		1006
B 1310.5
(-1.1)
P 		1422.2
(0.6)
P 		1390.1
(0.1)
P 		1185.2
(-3)
P 		1575.7
(3)
P 		1307.1
(-1.1)
P 		391
C 1666
(3)
P 		1319.5
(0.4)
P 		1424
(1.2)
P 		870.1
(-3)
P 		1470
(1.5)
P 		1189.4
(-0.6)
P 		796
avr 1196.5
(-1.8) 		1453.6
(3) 		1319.8
(0.5) 		1157
(-2.5) 		1374.5
(1.5) 		1132.7
(-3) 		321
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193168_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1302
Sequence Type Exemplar sequence
Representative Public ID F22D6.4 NCBI
Target Description F22D6.4 /REP_DB=WormBase Gene ID /WP=CE05685 /TR=SW:Q19724 /GB=CAA95810.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=NADH_ubiquinone oxidoreductase subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059606(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059606 NCBI Caenorhabditis elegans F22D6.4 (F22D6.4) mRNA, complete cds. 11/11 None
SNAP00000037650 ENSEMBL cdna:SNAP chromosome:CEL140:I:7098874:7099389:1 11/11 None
GENEFINDER00000037661 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7098874:7099389:1 11/11 None
F22D6.4.2 ENSEMBL cdna:known chromosome:CEL140:I:7098866:7099480:1 gene:F22D6.4 11/11 None
F22D6.4.1 ENSEMBL cdna:known chromosome:CEL140:I:7098872:7099497:1 gene:F22D6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7098804-7099320(+) 100.0 100.0
Public Domain and Genome References
Gene Title NADH_ubiquinone oxidoreductase subunit
Ensembl F22D6.4
Entrez Gene 184827 Entrez gene
SwissProt Q19724 EMBL-EBI
WormBase CE05685 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA95810 Hypothetical protein F22D6.4 [Caenorhabditis elegans] ref|NP_492007.1| F22D6.4 [Caenorhabditis elegans] sp|Q19724|NUMM_CAEEL Probable NADH-ubiquinone oxidoreductase 13 kDa-A subunit, mitochondrial precursor (Complex I-13KD-A) (CI-13KD-A) 6.0E-81
blast CAE73098 Hypothetical protein CBG20477 [Caenorhabditis briggsae] 6.0E-76
Sequence
Target Sequence 
>C. ELEGANS:193168_AT
tgaaccggcttcttcaacagtcggtttcacgcggacctcttgccgtccgagcttcttcta
caattacaacaaagccaccaactccgaatcaaattaccaaaaacaatgcacaattcgaca
aggtgactcacaccggacaagcgtgggatcaatctgattatcgtcttcaaagatttgata
tttcgaagaaaagcgtcaatccaaatgtagcaatgcatcttattgatcaacgccctccag
aggattgcggggataagagagttgtcttctgtgatggaggacatccagctcttggacatc
cgaaagtatacatcaaccttgacaaaccaggagttcatgcttgcggatattgtggaaacc
gattctataactcgcacgccacgaaggcagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAACCGGCTTCTTCAACAGTCGGT 551 571 14 Antisense
TTCAACAGTCGGTTTCACGCGGACC 168 683 26 Antisense
GCCACCAACTCCGAATCAAATTACC 140 281 87 Antisense
GACTCACACCGGACAAGCGTGGGAT 21 375 138 Antisense
GATCAATCTGATTATCGTCTTCAAA 642 421 160 Antisense
ATGCATCTTATTGATCAACGCCCTC 472 43 226 Antisense
CAACGCCCTCCAGAGGATTGCGGGG 268 187 241 Antisense
ACATCCAGCTCTTGGACATCCGAAA 518 103 294 Antisense
AAACCAGGAGTTCATGCTTGCGGAT 444 125 337 Antisense
AAACCGATTCTATAACTCGCACGCC 530 125 369 Antisense
TAACTCGCACGCCACGAAGGCAGAG 463 637 381 Antisense
Sequence Source Affymetrix Proprietary Database