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[193151_at] Zinc finger, C3HC4 type (RING finger)

Description F08B12.2 /REP_DB=WormBase Gene ID /WP=CE03163 /TR=SW:Q19189 /GB=CAA92113.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=Zinc finger, C3HC4 type (RING finger)
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 56      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 83      6grp_total > 57      4grp_total > 01      6grp_highest_sub > Cu      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 787
1 2 3 4 range
A 531.7
(-0.8)
P 		331.1
(-3)
P 		545.4
(-0.7)
P 		884.7
(3)
P 		554
B 522.6
(-2)
P 		399.5
(-3)
P 		528.6
(-1.9)
P 		1118.3
(3)
P 		719
C 528.2
(-1.9)
P 		423.7
(-3)
P 		625.9
(-1)
P 		1018.4
(3)
P 		595
avr 527.5
(-1.6) 		384.8
(-3) 		566.6
(-1.2) 		1007.1
(3) 		622

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 578
1 2 3 4 5 6 range
A 574.7
(0.4)
P 		488.7
(-0.7)
P 		524.4
(-0.2)
P 		456.3
(-1.1)
P 		316.8
(-3)
P 		766.8
(3)
P 		450
B 579.8
(0.8)
P 		458.2
(-1.5)
P 		378.5
(-3)
P 		405.4
(-2.5)
P 		395.7
(-2.7)
P 		693
(3)
P 		315
C 507
(-0.5)
P 		446.7
(-1.1)
P 		566.8
(0.2)
P 		390.5
(-1.7)
P 		261.5
(-3)
P 		839.3
(3)
P 		578
avr 553.8
(0.1) 		464.5
(-1.1) 		489.9
(-0.8) 		417.4
(-1.7) 		324.7
(-3) 		766.4
(3) 		442
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 193.6
(3)
P 		71.9
(-3)
P 		142.6
(0.5)
P 		129.5
(-0.2)
P 		122
B 166.6
(-0.1)
P 		98.7
(-3)
P 		122.5
(-2)
P 		238.3
(3)
P 		140
C 174.9
(0.3)
P 		121.8
(-3)
P 		218.2
(3)
P 		171.7
(0.1)
P 		96
avr 178.4
(2.9) 		97.5
(-3) 		161.1
(1.6) 		179.8
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 105.9
(-2.3)
P 		173.8
(2.2)
P 		114.2
(-1.8)
P 		115.7
(-1.7)
P 		95.6
(-3)
P 		186.7
(3)
P 		91
B 201.8
(1)
P 		152.5
(-0.8)
P 		91.9
(-3)
P 		107.4
(-2.4)
P 		160.8
(-0.5)
P 		258.4
(3)
P 		167
C 187.9
(0.2)
P 		136.3
(-1.3)
P 		144.3
(-1.1)
P 		78.5
(-3)
P 		84.6
(-2.8)
P 		281
(3)
P 		203
avr 165.2
(-0.3) 		154.2
(-0.7) 		116.8
(-2.3) 		100.5
(-3) 		113.7
(-2.4) 		242
(3) 		142
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193151_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17200
Sequence Type Exemplar sequence
Representative Public ID F08B12.2 NCBI
Target Description F08B12.2 /REP_DB=WormBase Gene ID /WP=CE03163 /TR=SW:Q19189 /GB=CAA92113.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=Zinc finger, C3HC4 type (RING finger)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077507(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077507 NCBI Caenorhabditis elegans PeRoXisome assembly factor family member (prx-12) (prx-12) mRNA, complete cds. 11/11 None
SNAP00000001945 ENSEMBL cdna:SNAP chromosome:CEL140:X:11393225:11394492:1 11/11 None
GENEFINDER00000001954 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11393225:11394492:1 10/11 None
F08B12.2 ENSEMBL cdna:known chromosome:CEL140:X:11393225:11394537:1 gene:F08B12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11393224-11394492(+) 100.0 100.0
Public Domain and Genome References
Gene Title Zinc finger, C3HC4 type (RING finger)
Gene Symbol prx-12
Ensembl F08B12.2
Entrez Gene 181331 Entrez gene
SwissProt Q19189 EMBL-EBI
WormBase CE03163 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258627_AT Pex2/Pex12 N-terminal domain-containing protein at
CANINE_2:CFAAFFX.27953.1.S1_AT similar to Peroxisome assembly protein 12 (Peroxin-12) (Peroxisome assembly factor-3) (PAF-3) cfa
CHICKEN:GGA.8242.1.S1_AT peroxisomal biogenesis factor 12 gga
HG-U133_PLUS_2:205094_AT peroxisomal biogenesis factor 12 hs
HG-U133A:205094_AT peroxisomal biogenesis factor 12 hs
HG-FOCUS:205094_AT peroxisomal biogenesis factor 12 hs
HG-U133A_2:205094_AT peroxisomal biogenesis factor 12 hs
HUGENEFL:U91521_AT peroxisomal biogenesis factor 12 hs
U133_X3P:G4505720_3P_AT peroxisomal biogenesis factor 12 hs
HG-U95AV2:35157_AT peroxisomal biogenesis factor 12 hs
MG-U74BV2:110333_AT peroxisomal biogenesis factor 12 mm
MOE430A:1416259_AT peroxisomal biogenesis factor 12 mm
MOUSE430A_2:1416259_AT peroxisomal biogenesis factor 12 mm
MOUSE430_2:1416259_AT peroxisomal biogenesis factor 12 mm
MOE430B:1458971_AT Peroxisomal biogenesis factor 12 (Pex12), mRNA mm
MOUSE430_2:1458971_AT Peroxisomal biogenesis factor 12 (Pex12), mRNA mm
MU19KSUBA:TC17967_AT Peroxisomal biogenesis factor 12 (Pex12), mRNA mm
MU19KSUBA:TC21033_AT Peroxisomal biogenesis factor 12 (Pex12), mRNA mm
MU19KSUBB:TC34870_AT Peroxisomal biogenesis factor 12 (Pex12), mRNA mm
MU19KSUBC:TC37508_AT Peroxisomal biogenesis factor 12 (Pex12), mRNA mm
RT-U34:AB002111_AT peroxisomal biogenesis factor 12 rn
RG-U34A:AB002111_AT peroxisomal biogenesis factor 12 rn
RAE230A:1369070_AT peroxisomal biogenesis factor 12 rn
RAT230_2:1369070_AT peroxisomal biogenesis factor 12 rn
Protein Similarities
Method ID Description E-Value
blast CAA92113 Hypothetical protein F08B12.2 [Caenorhabditis elegans] ref|NP_509908.1| PeRoXisome assembly factor family member (prx-12) [Caenorhabditis elegans] sp|Q19189|PEX12_CAEEL Putative peroxisome assembly protein 12 (Peroxin-12) 0.0
blast CAE70646 Hypothetical protein CBG17349 [Caenorhabditis briggsae] 1.0E-180
blast CAE70646 Hypothetical protein CBG17349 [Caenorhabditis briggsae] 1.0E-177
blast XP_582391 PREDICTED: similar to Peroxisome assembly protein 12 (Peroxin-12) (Peroxisome assembly factor 3) (PAF-3) [Bos taurus] 1.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR006845 EMBL-EBI Pex2 / Pex12, N-terminal 5.8E-89
Pfam IPR006845 EMBL-EBI Pex2 / Pex12, N-terminal 9.7E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509908.1 1 243-265
F08B12.2 1 243-265
SNAP00000001945 1 243-265
GENEFINDER00000001954 1 225-247
Sequence
Target Sequence 
>C. ELEGANS:193151_AT
tgcatacattttgaaccgcagctccattcactcgccatggctttacttctcaggcgtgat
tctgaaacatcttacgccagaagacttggaagctttcaatgctgttcctcttcatctcca
aactgggtatcaaatttctagaggaacattaaacgaacatattcatttaagattcttcaa
tcgcatctggagatttattttgggactacctggaatcgtttcaaggctctttgcttatgg
gctcttctttgtacagtttttggattatatgtacaacacagatttggcaaaacttacaaa
gactggccttgacggagctattccttcacctccacacaaaatgatcatatctgaatcgga
aattttgagtttggacaccaataaatgcccaatttgtttgaagaaaagagttaatgatac
tgcgctttttgtatctggatatgtattctgctacacatgtatcaaccaatacgtaaacac
ttataacaaatgtccagtgacaggatgcccggcaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCATACATTTTGAACCGCAGCTCC 397 577 546 Antisense
TCGCCATGGCTTTACTTCTCAGGCG 359 599 577 Antisense
GAAGCTTTCAATGCTGTTCCTCTTC 461 341 634 Antisense
TCCTCTTCATCTCCAAACTGGGTAT 154 591 651 Antisense
TTGGGACTACCTGGAATCGTTTCAA 542 545 745 Antisense
TCAAGGCTCTTTGCTTATGGGCTCT 289 633 766 Antisense
TATGGGCTCTTCTTTGTACAGTTTT 378 655 781 Antisense
AAGACTGGCCTTGACGGAGCTATTC 543 151 844 Antisense
TCCTTCACCTCCACACAAAATGATC 383 591 867 Antisense
ACTGCGCTTTTTGTATCTGGATATG 192 99 964 Antisense
TCCAGTGACAGGATGCCCGGCAAAC 1 589 1038 Antisense
Sequence Source Affymetrix Proprietary Database