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[193127_s_at] methylenetetrahydrofolate dehydrogenase

Description K07E3.3 /REP_DB=WormBase Gene ID /WP=CE25047 /TR=Q21285 /GB=AAC69100.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=methylenetetrahydrofolate dehydrogenase
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 72      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 44      6grp_total > 73      4grp_total > 09      6grp_highest_sub > 04      6grp_cgc > 75      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1976
1 2 3 4 range
A 622
(-3)
P 		1057.2
(-1.7)
P 		1346.3
(-0.8)
P 		2598.2
(3)
P 		1976
B 741.2
(-3)
P 		1047.4
(-2)
P 		987.2
(-2.2)
P 		2494.2
(3)
P 		1753
C 636.3
(-3)
P 		982.2
(-1.9)
P 		1055.6
(-1.6)
P 		2499.2
(3)
P 		1863
avr 666.5
(-3) 		1028.9
(-1.8) 		1129.7
(-1.5) 		2530.5
(3) 		1864

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1821
1 2 3 4 5 6 range
A 950
(-3)
P 		1729.1
(-0.2)
P 		2138.9
(1.3)
P 		2616.9
(3)
P 		1980.7
(0.7)
P 		1536.1
(-0.9)
P 		1667
B 795.6
(-3)
P 		1397.3
(-0.5)
P 		2264.8
(3)
P 		2164.9
(2.6)
P 		1851
(1.3)
P 		1308.5
(-0.9)
P 		1469
C 942
(-3)
P 		1445.4
(-1.1)
P 		2535.7
(3)
P 		2468.6
(2.7)
P 		1731.4
(-0)
P 		1725.4
(-0.1)
P 		1594
avr 895.9
(-3) 		1523.9
(-0.5) 		2313.1
(2.6) 		2416.8
(3) 		1854.4
(0.8) 		1523.3
(-0.5) 		1521
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 321
1 2 3 4 range
A 226.5
(-3)
P 		229.5
(-2.9)
P 		352
(1.9)
P 		380.3
(3)
P 		154
B 236.4
(-2.8)
P 		258.7
(-2.4)
P 		228.8
(-3)
P 		531.5
(3)
P 		303
C 210.6
(-3)
P 		282.4
(-1)
P 		367.9
(1.5)
P 		421.4
(3)
P 		211
avr 224.5
(-3) 		256.9
(-2.1) 		316.2
(-0.5) 		444.4
(3) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 577
1 2 3 4 5 6 range
A 175.1
(-3)
P 		615
(2.4)
P 		465.8
(0.6)
P 		663.7
(3)
P 		597.7
(2.2)
P 		374
(-0.6)
P 		489
B 276.8
(-3)
P 		465.1
(-0.6)
P 		550
(0.4)
P 		573.4
(0.7)
P 		752
(3)
P 		487.9
(-0.3)
P 		475
C 349.2
(-3)
P 		441
(-1.1)
P 		645.4
(3)
P 		496.4
(-0)
P 		560.3
(1.3)
P 		577.6
(1.6)
P 		296
avr 267
(-3) 		507
(0.9) 		553.7
(1.7) 		577.8
(2) 		636.7
(3) 		479.8
(0.5) 		370
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193127_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16169
Sequence Type Exemplar sequence
Representative Public ID K07E3.3 NCBI
Target Description K07E3.3 /REP_DB=WormBase Gene ID /WP=CE25047 /TR=Q21285 /GB=AAC69100.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=methylenetetrahydrofolate dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076961(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076961 NCBI Caenorhabditis elegans Dauer or Aging adult Overexpression family member (dao-3) (dao-3) mRNA, complete cds. 11/11 None
SNAP00000041514 ENSEMBL cdna:SNAP chromosome:CEL140:X:8080955:8086489:-1 11/11 None
GENEFINDER00000041526 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8080955:8086489:-1 11/11 None
K07E3.3 ENSEMBL cdna:known chromosome:CEL140:X:8084162:8086490:-1 gene:K07E3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8084351-8086488(-) 100.0 100.0
Public Domain and Genome References
Gene Title methylenetetrahydrofolate dehydrogenase
Gene Symbol dao-3
Ensembl K07E3.3
Entrez Gene 181065 Entrez gene
SwissProt Q21285 EMBL-EBI
WormBase CE25047 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15516_S_AT tetrahydrofolate dehydrogenase/cyclohydrolase, putative at
ATGENOME1:15515_R_AT tetrahydrofolate dehydrogenase/cyclohydrolase, putative at
ATH1-121501:255685_S_AT tetrahydrofolate dehydrogenase/cyclohydrolase, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9396 folic acid and derivative biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69101 Hypothetical protein K07E3.4b [Caenorhabditis elegans] gb|AAC69104.1| Hypothetical protein K07E3.4a [Caenorhabditis elegans] ref|NP_509360.1| K07E3.4b [Caenorhabditis elegans] ref|NP_509361.1| K07E3.4a [Caenorhabditis elegans] 0.0
blast NP_001008007 mthfd1-prov protein [Xenopus tropicalis] gb|AAH80885.1| Mthfd1-prov protein [Xenopus tropicalis] 0.0
blast P11586 C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ; Formyltetrahydrofolate synthetase ] gb|AAA59574.1| MDMCSF (EC 1.5.1.5; EC 3.5.4.9; EC 6.3.4.3) 0.0
blast AAC69100 Dauer or aging adult overexpression protein 3 [Caenorhabditis elegans] ref|NP_509362.1| Dauer or Aging adult Overexpression family member (dao-3) [Caenorhabditis elegans] 1.0E-173
blast CAE70348 Hypothetical protein CBG16896 [Caenorhabditis briggsae] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR000672 EMBL-EBI Tetrahydrofolate dehydrogenase/cyclohydrolase 3.0E-66
Pfam IPR000672 EMBL-EBI Tetrahydrofolate dehydrogenase/cyclohydrolase 8.0E-103
Pfam IPR000559 EMBL-EBI Formate-tetrahydrofolate ligase, FTHFS 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:193127_S_AT
tatcattcagcttccactggactgcaagcacgaaatcgacgcagactctgttatcgactt
gattgacccattgaaagatgtcgatggactgactcgtatcaatgctggacgtttggctcg
tggagaactccagcgtacgatctttccttgcactccgtttggatgcttgtacttggttca
acaggcaactggtgatgccaactttgtgtctggaaaggaggtggttgttttgggacgctc
gaaaattgtcggatctccagccgctgcgcttttcttgtggcatcatggaactgtgacaat
ctgccactcaaaaactccaaatctcaaggagaaatgcctgagagcagatattttgattgt
ggcaattgggagaaaacatttcgtcaaagctgattggatcaagcctggcgcattcgtcat
cgactgtggaatcaacgttggtgacgacccaaactcacgcaaaatttacggagatgttga
cacagaagccgcaaaggaagtcgccggattcctcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCATTCAGCTTCCACTGGACTGC 343 659 300 Antisense
ACGCAGACTCTGTTATCGACTTGAT 310 89 338 Antisense
GGACTGACTCGTATCAATGCTGGAC 130 525 385 Antisense
ATGCTGGACGTTTGGCTCGTGGAGA 230 41 401 Antisense
CCTTGCACTCCGTTTGGATGCTTGT 473 269 445 Antisense
GCGCTTTTCTTGTGGCATCATGGAA 688 283 565 Antisense
GGAACTGTGACAATCTGCCACTCAA 112 531 586 Antisense
GCCACTCAAAAACTCCAAATCTCAA 474 279 602 Antisense
TGGATCAAGCCTGGCGCATTCGTCA 676 549 694 Antisense
TTGGTGACGACCCAAACTCACGCAA 494 707 737 Antisense
AAAGGAAGTCGCCGGATTCCTCACA 467 121 792 Antisense
Sequence Source Affymetrix Proprietary Database