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[193124_s_at]

Description C07A12.4 /REP_DB=WormBase Gene ID /WP=CE03972 /GEN=pdi-2 /TR=Q17770 /GB=AAK39152.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=protein disulfide isomerase
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 01      4grp_highest_8_8 > 09      4grp_high_8_8 > 54      6grp_highest_nonorm > 06      6grp_total > 23      4grp_total > 57      6grp_cgc > 70      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12609
1 2 3 4 range
A 7231.4
(-1.6)
P 		16484.1
(3)
P 		16390.2
(3)
P 		4508.3
(-3)
P 		11976
B 7526
(-1.6)
P 		13782.6
(1.7)
P 		16352.3
(3)
P 		4798.8
(-3)
P 		11554
C 6828.3
(-1.5)
P 		15587.7
(3)
P 		15585.6
(3)
P 		3874.7
(-3)
P 		11713
avr 7195.2
(-1.6) 		15284.8
(2.6) 		16109.4
(3) 		4393.9
(-3) 		11716

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9416
1 2 3 4 5 6 range
A 9662.3
(3)
P 		9122.2
(2.4)
P 		7733.8
(0.7)
P 		8341.8
(1.4)
P 		6539.1
(-0.7)
P 		4595.6
(-3)
P 		5067
B 10757.7
(3)
P 		8316.3
(0.9)
P 		9980.3
(2.3)
P 		7909
(0.6)
P 		6194.5
(-0.8)
P 		3620
(-3)
P 		7138
C 12247.6
(3)
P 		7934.7
(0.3)
P 		8714.5
(0.7)
P 		6363.5
(-0.7)
P 		8164.2
(0.4)
P 		2831.8
(-3)
P 		9416
avr 10889.2
(3) 		8457.7
(1) 		8809.5
(1.3) 		7538.1
(0.2) 		6965.9
(-0.3) 		3682.5
(-3) 		7207
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4779
1 2 3 4 range
A 2632.9
(0.3)
P 		3578.3
(1.8)
P 		4284.7
(3)
P 		659.9
(-3)
P 		3625
B 2400
(-0)
P 		3404
(2.2)
P 		3789.2
(3)
P 		1022.6
(-3)
P 		2767
C 2260.3
(-1)
P 		4481.5
(1.8)
P 		5432.1
(3)
P 		653.3
(-3)
P 		4779
avr 2431.1
(-0.3) 		3821.3
(1.9) 		4502
(3) 		778.6
(-3) 		3723

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3592
1 2 3 4 5 6 range
A 1781.2
(-1.1)
P 		3244.3
(3)
P 		1684.3
(-1.4)
P 		2115.7
(-0.2)
P 		1973.2
(-0.6)
P 		1118.9
(-3)
P 		2125
B 3743.1
(3)
P 		2768.2
(0.6)
P 		2423.5
(-0.3)
P 		2094.8
(-1.1)
P 		2516.8
(-0.1)
P 		1349.9
(-3)
P 		2393
C 4539.6
(3)
P 		2421.1
(-0.5)
P 		2218
(-0.9)
P 		1279.7
(-2.4)
P 		2641.8
(-0.2)
P 		948
(-3)
P 		3592
avr 3354.6
(3) 		2811.2
(1.5) 		2108.6
(-0.4) 		1830.1
(-1.1) 		2377.3
(0.4) 		1138.9
(-3) 		2216
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193124_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16161
Sequence Type Exemplar sequence
Representative Public ID C07A12.4 NCBI
Target Description C07A12.4 /REP_DB=WormBase Gene ID /WP=CE03972 /GEN=pdi-2 /TR=Q17770 /GB=AAK39152.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=protein disulfide isomerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076377(11), NM_182439(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076377 NCBI Caenorhabditis elegans Protein Disulfide Isomerase family member (pdi-2) (pdi-2) mRNA, complete cds. 11/11 None
NM_182439 NCBI Caenorhabditis elegans Protein Disulfide Isomerase family member (pdi-2) (pdi-2) mRNA, complete cds. 11/11 None
SNAP00000032345 ENSEMBL cdna:SNAP chromosome:CEL140:X:4524209:4526174:1 11/11 None
GENEFINDER00000032353 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4524209:4526174:1 11/11 None
C07A12.4a.1 ENSEMBL cdna:known chromosome:CEL140:X:4524157:4526505:1 gene:C07A12.4 11/11 None
C07A12.4a.2 ENSEMBL cdna:known chromosome:CEL140:X:4524161:4526502:1 gene:C07A12.4 11/11 None
C07A12.4b ENSEMBL cdna:known chromosome:CEL140:X:4524209:4526174:1 gene:C07A12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4524209-4526175(+) 100.0 100.0
Public Domain and Genome References
Ensembl C07A12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39152 Protein disulfide isomerase protein 2, isoform a [Caenorhabditis elegans] ref|NP_508778.1| Protein Disulfide Isomerase family member (pdi-2) [Caenorhabditis elegans] sp|Q17770|PDI2_CAEEL Protein disulfide-isomerase 2 precursor (PDI 1) (Prolyl 4-hydroxylase beta subunit) 0.0
blast S71862 protein disulfide-isomerase (EC 5.3.4.1) precursor - Caenorhabditis elegans (fragment) 0.0
blast CAE68606 Hypothetical protein CBG14484 [Caenorhabditis briggsae] 0.0
blast AAN39682 Protein disulfide isomerase protein 2, isoform b [Caenorhabditis elegans] ref|NP_872239.1| Protein Disulfide Isomerase family member (pdi-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.9E-52
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 18.0
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.0E-39
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 18.0
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.0E-39
Sequence
Target Sequence 
>C. ELEGANS:193124_S_AT
gagaacatctccaagttcacccaaaactaccttgatggatccgtcaagccacaccttatg
tccgaagatatcccagaggattgggacaagaacccagtcaagatccttgtcggaaagaac
ttcgagcaagtcgcccgtgacaacaccaagaacgttcttgttgagttctacgccccatgg
tgcggacactgcaagcaactcgccccaacctgggacaagctcggagagaagttcgctgac
gacgagagcattgtcatcgccaagatggactcaaccctcaacgaggtcgaggatgtcaag
atccaatctttcccaaccatcaagttcttcccagctggatccaacaaggtcgtcgactac
accggagaccgtaccattgaaggattcacaaagttcctcgagaccaacggaaaggaggga
gctggagcttccgaggaggaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAACATCTCCAAGTTCACCCAAA 164 393 1009 Antisense
AAGCCACACCTTATGTCCGAAGATA 329 149 1054 Antisense
GAACCCAGTCAAGATCCTTGTCGGA 167 345 1098 Antisense
AAAGAACTTCGAGCAAGTCGCCCGT 659 119 1122 Antisense
CCAACCTGGGACAAGCTCGGAGAGA 83 277 1213 Antisense
GACGACGAGAGCATTGTCATCGCCA 528 379 1246 Antisense
GGACTCAACCCTCAACGAGGTCGAG 409 525 1275 Antisense
GCTGGATCCAACAAGGTCGTCGACT 239 295 1342 Antisense
CACCGGAGACCGTACCATTGAAGGA 63 201 1368 Antisense
GATTCACAAAGTTCCTCGAGACCAA 309 431 1391 Antisense
GGAGCTGGAGCTTCCGAGGAGGAGA 626 521 1426 Antisense
Sequence Source Affymetrix Proprietary Database