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[193122_at] ubiquitin carboxyl-terminal hydrolase

Description K02C4.3 /REP_DB=WormBase Gene ID /WP=CE01599 /TR=SW:Q09931 /GB=CAA87786.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ubiquitin carboxyl-terminal hydrolase
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 36      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 82      6grp_total > 37      4grp_total > 55      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 242
1 2 3 4 range
A 407.6
(3)
P 		274.6
(-1.9)
P 		245.8
(-3)
P 		249.7
(-2.9)
P 		162
B 416.5
(3)
P 		214.4
(-2)
P 		255.8
(-1)
P 		174.1
(-3)
P 		242
C 352.9
(3)
P 		198.1
(-3)
P 		272.3
(-0.1)
P 		276.7
(0)
P 		155
avr 392.3
(3) 		229
(-3) 		258
(-1.9) 		233.5
(-2.8) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 258
1 2 3 4 5 6 range
A 366.8
(-0.2)
P 		351.2
(-0.8)
P 		290.5
(-2.9)
P 		456.6
(3)
P 		288.8
(-3)
P 		410.1
(1.3)
P 		168
B 432.7
(1.9)
P 		479.9
(3)
P 		272.5
(-1.8)
P 		222.2
(-3)
P 		328
(-0.5)
P 		351.1
(0)
P 		258
C 442.5
(3)
P 		353.9
(-0.4)
P 		368.4
(0.1)
P 		330.4
(-1.3)
P 		287.3
(-3)
P 		329.7
(-1.4)
P 		155
avr 414
(3) 		395
(2) 		310.5
(-2.5) 		336.4
(-1.1) 		301.4
(-3) 		363.6
(0.3) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 148.4
(3)
P 		59.6
(-1.8)
P 		64.2
(-1.5)
P 		36.5
(-3)
P 		112
B 132.8
(3)
P 		52.9
(-2)
P 		59.3
(-1.6)
P 		37.1
(-3)
P 		96
C 116.8
(3)
P 		57
(-2.1)
P 		94.9
(1.1)
P 		46.7
(-3)
P 		70
avr 132.7
(3) 		56.5
(-1.9) 		72.8
(-0.9) 		40.1
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 67.6
(-2.6)
P 		124.9
(3)
P 		63.3
(-3)
P 		115.8
(2.1)
P 		87.2
(-0.7)
P 		99.8
(0.6)
P 		62
B 150.5
(2.5)
P 		159.7
(3)
P 		66.2
(-2.6)
P 		58.8
(-3)
P 		133.3
(1.4)
P 		130.9
(1.3)
P 		101
C 164
(3)
P 		108
(-0.4)
P 		93.8
(-1.3)
P 		66.4
(-3)
P 		93
(-1.4)
P 		110.4
(-0.3)
P 		98
avr 127.4
(2.6) 		130.9
(3) 		74.4
(-3) 		80.3
(-2.4) 		104.5
(0.2) 		113.7
(1.2) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193122_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2642
Sequence Type Exemplar sequence
Representative Public ID K02C4.3 NCBI
Target Description K02C4.3 /REP_DB=WormBase Gene ID /WP=CE01599 /TR=SW:Q09931 /GB=CAA87786.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ubiquitin carboxyl-terminal hydrolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063295(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063295 NCBI Caenorhabditis elegans K02C4.3 (K02C4.3) mRNA, complete cds. 11/11 None
SNAP00000018322 ENSEMBL cdna:SNAP chromosome:CEL140:II:8080992:8086768:-1 11/11 None
GENEFINDER00000018328 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8081080:8086768:-1 11/11 None
K02C4.3 ENSEMBL cdna:known chromosome:CEL140:II:8080747:8087107:-1 gene:K02C4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8081139-8087104(-) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin carboxyl-terminal hydrolase
Ensembl K02C4.3
Entrez Gene 174300 Entrez gene
SwissProt Q09931 EMBL-EBI
WormBase CE01599 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4221 ubiquitin thiolesterase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87786 Hypothetical protein K02C4.3 [Caenorhabditis elegans] ref|NP_495696.1| K02C4.3 [Caenorhabditis elegans] sp|Q09931|UBPY_CAEEL Probable ubiquitin carboxyl-terminal hydrolase K02C4.3 (Ubiquitin thiolesterase) (Ubiquitin-specific processing protease) (Deubiquitinating enzyme) 0.0
blast CAE57727 Hypothetical protein CBG00738 [Caenorhabditis briggsae] 0.0
blast XP_221722 PREDICTED: similar to Ubiquitin specific protease 25 [Rattus norvegicus] 9.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 6.9E-116
Pfam IPR001394 EMBL-EBI Peptidase C19, ubiquitin carboxyl-terminal hydrolase family 2 3.9E-115
Sequence
Target Sequence 
>C. ELEGANS:193122_AT
gacgtattgtgcgatgattggcgccagaaatgcacgtatctgcaatggattattgagaga
aatggcatattttttggaatcgtattcaattttttttgtcagccaaaaacacattgaagc
ttgcacagttttctcaacgattacaatgattaaactaatcatgcaacatatggcatctaa
aacgcttcaactcattgacatggaattccatttatcgaaaaatgaacgtcgagttagatt
tgaagatataatcagggaagtggtatcgtcggtatgcatcattcatcactggtctaaaag
ttattcgaaagaaaatgaaatcaacttgaaagaactgatgggtctcctccatttgaaagt
cgagtttatggtttcgctcacttcgttcgaagtcgcagatccgaaatacgaatgcttaca
agcgtttaaggcaatggttgtgaacaccgtgatggacctagaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGTATTGTGCGATGATTGGCGCC 342 377 3303 Antisense
GATTGGCGCCAGAAATGCACGTATC 611 435 3318 Antisense
GAAGCTTGCACAGTTTTCTCAACGA 362 341 3418 Antisense
GGCATCTAAAACGCTTCAACTCATT 135 537 3474 Antisense
GGAAGTGGTATCGTCGGTATGCATC 500 531 3558 Antisense
GGTATGCATCATTCATCACTGGTCT 318 505 3573 Antisense
AAGAACTGATGGGTCTCCTCCATTT 233 151 3632 Antisense
GGGTCTCCTCCATTTGAAAGTCGAG 78 497 3642 Antisense
CGAGTTTATGGTTTCGCTCACTTCG 355 249 3663 Antisense
ACTTCGTTCGAAGTCGCAGATCCGA 599 103 3682 Antisense
GTGAACACCGTGATGGACCTAGAAC 33 483 3742 Antisense
Sequence Source Affymetrix Proprietary Database