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[193111_s_at] acetyl-coenzyme A synthetase

Description K03A1.5 /REP_DB=WormBase Gene ID /WP=CE04713 /GEN=sur-5 /TR=Q21166 /GB=AAA83327.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=acetyl-coenzyme A synthetase
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 52      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 34      6grp_total > 52      4grp_total > 59      6grp_highest_sub > 03      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1162
1 2 3 4 range
A 1381.9
(0.6)
P 		1612.5
(1.9)
P 		1795.7
(3)
P 		757.5
(-3)
P 		1038
B 1404.2
(-0.3)
P 		1509
(0.4)
P 		1919.8
(3)
P 		976
(-3)
P 		944
C 1350
(-0.2)
P 		1500.6
(0.8)
P 		1829.9
(3)
P 		939.4
(-3)
P 		891
avr 1378.7
(0.1) 		1540.7
(1.1) 		1848.5
(3) 		891
(-3) 		958

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3835
1 2 3 4 5 6 range
A 1155.9
(-2.5)
P 		2603
(1.6)
P 		3069.8
(3)
P 		2291.7
(0.7)
P 		1187.9
(-2.4)
P 		997.2
(-3)
P 		2073
B 1142.7
(-2.5)
P 		2251.1
(-0.6)
P 		4384.5
(3)
P 		2405.4
(-0.4)
P 		1185.6
(-2.4)
P 		858.8
(-3)
P 		3526
C 1287.9
(-2.5)
P 		2319.9
(-0.8)
P 		4693.6
(3)
P 		2411.8
(-0.7)
P 		1320.4
(-2.4)
P 		966.4
(-3)
P 		3727
avr 1195.5
(-2.5) 		2391.3
(-0.2) 		4049.3
(3) 		2369.6
(-0.2) 		1231.3
(-2.4) 		940.8
(-3) 		3109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 527
1 2 3 4 range
A 503.1
(3)
P 		350
(0.7)
P 		469.4
(2.5)
P 		110.9
(-3)
P 		392
B 447.8
(3)
P 		372.7
(1.1)
P 		444.9
(2.9)
P 		208
(-3)
P 		240
C 446.9
(0.6)
P 		431.4
(0.4)
P 		637.8
(3)
P 		158.4
(-3)
P 		479
avr 465.9
(2.1) 		384.7
(0.8) 		517.4
(3) 		159.1
(-3) 		358

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 982
1 2 3 4 5 6 range
A 213.1
(-3)
P 		925.8
(3)
P 		668.5
(0.8)
P 		581.2
(0.1)
P 		358.4
(-1.8)
P 		242.8
(-2.7)
P 		713
B 397.6
(-2.4)
P 		749.3
(0.5)
P 		1064.7
(3)
P 		637.1
(-0.4)
P 		481.7
(-1.7)
P 		320.2
(-3)
P 		745
C 477.4
(-1.9)
P 		707.9
(-0.4)
P 		1194.6
(3)
P 		485
(-1.9)
P 		427.3
(-2.3)
P 		323.5
(-3)
P 		871
avr 362.7
(-2.4) 		794.3
(1.4) 		975.9
(3) 		567.8
(-0.6) 		422.5
(-1.9) 		295.5
(-3) 		680
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 193111_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16685
Sequence Type Exemplar sequence
Representative Public ID K03A1.5 NCBI
Target Description K03A1.5 /REP_DB=WormBase Gene ID /WP=CE04713 /GEN=sur-5 /TR=Q21166 /GB=AAA83327.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=acetyl-coenzyme A synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076828(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076828 NCBI Caenorhabditis elegans SUppressor of activated let-60 Ras family member (sur-5) (sur-5) mRNA, complete cds. 11/11 A 
SNAP00000013866 ENSEMBL cdna:SNAP chromosome:CEL140:X:7301706:7305515:-1 11/11 None
GENEFINDER00000013874 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7301706:7305515:-1 11/11 None
K03A1.5 ENSEMBL cdna:known chromosome:CEL140:X:7301303:7305516:-1 gene:K03A1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7301704-7305514(-) 98.57 100.0
Public Domain and Genome References
Gene Title acetyl-coenzyme A synthetase
Gene Symbol sur-5
Ensembl K03A1.5
Entrez Gene 180992 Entrez gene
SwissProt Q21166 EMBL-EBI
WormBase CE04713 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.11289.1.S1_S_AT similar to acetoacetyl-CoA synthetase cfa
CANINE_2:CFAAFFX.11305.1.S1_AT similar to acetoacetyl-CoA synthetase cfa
CHICKEN:GGAAFFX.13153.1.S1_AT acetoacetyl-CoA synthetase gga
CHICKEN:GGAAFFX.1857.1.S1_S_AT acetoacetyl-CoA synthetase gga
HU35KSUBB:RC_N23638_AT acetoacetyl-CoA synthetase hs
HG-U133A:218434_S_AT acetoacetyl-CoA synthetase hs
HG-U133_PLUS_2:218434_S_AT acetoacetyl-CoA synthetase hs
HG-U133A_2:218434_S_AT acetoacetyl-CoA synthetase hs
U133_X3P:G12965198_3P_A_AT acetoacetyl-CoA synthetase hs
HG-U95B:47923_AT acetoacetyl-CoA synthetase hs
HG-U95C:57337_S_AT acetoacetyl-CoA synthetase hs
HG-U95C:61331_AT acetoacetyl-CoA synthetase hs
HG-U95C:61332_AT acetoacetyl-CoA synthetase hs
HG-U133_PLUS_2:236722_AT Acetoacetyl-CoA synthetase hs
HG-U133B:236722_AT Acetoacetyl-CoA synthetase hs
HG-U95E:77485_AT Acetoacetyl-CoA synthetase hs
U133_X3P:HS.169054.0.A1_3P_AT Acetoacetyl-CoA synthetase hs
MG-U74BV2:113164_AT acetoacetyl-CoA synthetase mm
MOE430A:1423797_AT acetoacetyl-CoA synthetase mm
MOUSE430A_2:1423797_AT acetoacetyl-CoA synthetase mm
MOUSE430_2:1423797_AT acetoacetyl-CoA synthetase mm
MOE430A:1456081_A_AT acetoacetyl-CoA synthetase mm
MOUSE430A_2:1456081_A_AT acetoacetyl-CoA synthetase mm
MOUSE430_2:1456081_A_AT acetoacetyl-CoA synthetase mm
MOE430A:1451116_AT acetoacetyl-CoA synthetase mm
MOUSE430A_2:1451116_AT acetoacetyl-CoA synthetase mm
MOUSE430_2:1451116_AT acetoacetyl-CoA synthetase mm
MU19KSUBB:TC31664_AT Acetoacetyl-CoA synthetase (Aacs), mRNA mm
RG-U34B:RC_AI009125_AT acetoacetyl-CoA synthetase rn
RAE230A:1368126_AT acetoacetyl-CoA synthetase rn
RAT230_2:1368126_AT acetoacetyl-CoA synthetase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
8299 isoprenoid biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16405 CoA-ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83327 Suppressor of activated let-60 ras protein 5 [Caenorhabditis elegans] ref|NP_509229.1| SUppressor of activated let-60 Ras family member (sur-5) [Caenorhabditis elegans] gb|AAM44123.1| SUR-5 [Caenorhabditis elegans] 0.0
blast CAE65870 Hypothetical protein CBG11014 [Caenorhabditis briggsae] 0.0
blast XP_543365 PREDICTED: similar to acetoacetyl-CoA synthetase [Canis familiaris] 1.0E-132
Protein Families
Method ID Description E-Value
ec AC2L_HUMAN (Q9NUB1) Acetyl-coenzyme A synthetase 2-like, mitochondrial precursor (EC 6.2.1.1) (Acetate--CoA ligase 2) (Acetyl-CoA synthetase 2) 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 2.2E-48
Sequence
Target Sequence 
>C. ELEGANS:193111_S_AT
agagttagtgtgtgtcaccccattcccctcaatgccatcacatttcttgaatgacacgga
tggcaagaagtaccgtgatgcctactttgctcgcctagaaccattttgggctcatggtga
ttttgtgagagtgaatcattctacgggcggtgtagaaatgctcggaagaagtgatgctac
attgaatcgcggcggagttcgaattggaacagctgagatttattcggttgttgaaaaaat
tccacatattgctgattgcattgtagctggaagacttgtcgaagagggcatggacgagga
agttttgctgtttgtgaaaatggttccgggtcaagagctcacacacagtatccaggcagc
cattgtttctaaactccggaacgacatgtccccgcgacatgttccaaacaaaatttacgc
agttgatgatattccgtatacttcaagcggaaagaaagtggaagttgccgttaagcaaat
tgtgagtggaaaggccgtccagaaagcgtcttcgattcgcaatcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGTTAGTGTGTGTCACCCCATTC 412 67 1554 Antisense
GAAGTACCGTGATGCCTACTTTGCT 642 333 1620 Antisense
CTTTGCTCGCCTAGAACCATTTTGG 130 221 1638 Antisense
GAGTGAATCATTCTACGGGCGGTGT 494 401 1682 Antisense
GATGCTACATTGAATCGCGGCGGAG 210 413 1726 Antisense
AATGGTTCCGGGTCAAGAGCTCACA 454 161 1872 Antisense
GCTCACACACAGTATCCAGGCAGCC 703 301 1890 Antisense
CCAGGCAGCCATTGTTTCTAAACTC 598 269 1905 Antisense
TAAACTCCGGAACGACATGTCCCCG 365 629 1923 Antisense
ACATGTCCCCGCGACATGTTCCAAA 451 107 1937 Antisense
GAAAGCGTCTTCGATTCGCAATCCA 636 359 2055 Antisense
Sequence Source Affymetrix Proprietary Database