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[193104_at]

Description T24D8.5 /REP_DB=WormBase Gene ID /WP=CE05009 /GEN=nlp-2 /TR=Q22739 /GB=AAA81446.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=C. tentans secretory protein
Cluster 6grp_high1_10_10 > 89      6grp_total > 96      4grp_total > 04      6grp_cgc > 69      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 525
1 2 3 4 range
A 156.1
(-1.6)
P 		93.5
(-2.3)
M 		36.4
(-3)
A 		539.6
(3)
P 		503
B 181.1
(-1.6)
P 		81.6
(-2.7)
A 		60.2
(-3)
A 		561.8
(3)
P 		502
C 163.5
(-1.3)
P 		65.9
(-2.8)
P 		50
(-3)
A 		459.7
(3)
P 		410
avr 166.9
(-1.5) 		80.3
(-2.6) 		48.9
(-3) 		520.4
(3) 		472

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 110.2
(-0.5)
P 		81.1
(-2.1)
P 		64.1
(-3)
P 		93.1
(-1.4)
P 		133.5
(0.8)
P 		174.8
(3)
P 		111
B 92.6
(-1)
P 		67.7
(-2)
M 		43
(-3)
A 		108.4
(-0.4)
P 		194.2
(3)
P 		189.4
(2.8)
P 		151
C 75.4
(-1.7)
P 		40.2
(-2.8)
A 		34.7
(-3)
A 		118.5
(-0.3)
P 		131.2
(0.1)
P 		223.6
(3)
P 		189
avr 92.7
(-1.2) 		63
(-2.4) 		47.3
(-3) 		106.7
(-0.6) 		153
(1.3) 		195.9
(3) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 56.8
(1.1)
P 		20.3
(-2.1)
M 		9.5
(-3)
A 		79
(3)
P 		70
B 57.8
(-0.5)
P 		20.1
(-2.6)
A 		13.9
(-3)
A 		119.7
(3)
P 		106
C 54.1
(0.7)
P 		18.9
(-2.9)
P 		17.4
(-3)
A 		77.5
(3)
P 		60
avr 56.2
(0.3) 		19.8
(-2.5) 		13.6
(-3) 		92.1
(3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 20.3
(-1.7)
P 		28.9
(0.1)
P 		14
(-3)
P 		23.6
(-1)
P 		40.3
(2.5)
P 		42.5
(3)
P 		29
B 32.2
(-1.1)
P 		22.5
(-1.9)
M 		10.5
(-3)
A 		28.7
(-1.4)
P 		78.9
(3)
P 		70.6
(2.3)
P 		68
C 27.9
(-1.3)
P 		12.3
(-2.7)
A 		8.8
(-3)
A 		23.8
(-1.6)
P 		42.5
(0.1)
P 		74.8
(3)
P 		66
avr 26.8
(-1.2) 		21.2
(-1.8) 		11.1
(-3) 		25.4
(-1.3) 		53.9
(2) 		62.6
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 193104_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18560
Sequence Type Exemplar sequence
Representative Public ID T24D8.5 NCBI
Target Description T24D8.5 /REP_DB=WormBase Gene ID /WP=CE05009 /GEN=nlp-2 /TR=Q22739 /GB=AAA81446.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=C. tentans secretory protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076025(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076025 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-2) (nlp-2) mRNA, complete cds. 11/11 None
SNAP00000017735 ENSEMBL cdna:SNAP chromosome:CEL140:X:2344052:2344678:-1 11/11 None
GENEFINDER00000017741 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2344052:2344678:-1 11/11 None
T24D8.5 ENSEMBL cdna:known chromosome:CEL140:X:2343994:2344721:-1 gene:T24D8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2344051-2344678(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-2
Ensembl T24D8.5
Entrez Gene 188857 Entrez gene
SwissProt Q22739 EMBL-EBI
WormBase CE05009 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81446 Neuropeptide-like protein protein 2 [Caenorhabditis elegans] ref|NP_508426.1| Neuropeptide-Like Protein family member (nlp-2) [Caenorhabditis elegans] 1.0E-82
blast AAA81446 Neuropeptide-like protein protein 2 [Caenorhabditis elegans] ref|NP_508426.1| Neuropeptide-Like Protein family member (nlp-2) [Caenorhabditis elegans] 5.0E-79
blast CAE58890 Hypothetical protein CBG02131 [Caenorhabditis briggsae] 3.0E-59
blast CAE58890 Hypothetical protein CBG02131 [Caenorhabditis briggsae] 1.0E-55
Sequence
Target Sequence 
>C. ELEGANS:193104_AT
tttgctttgctctgcgcagtttattctgaagcagttccgcttcaggtctatcgtcctgac
gaatcatcggctgtcgatgtggttgtccttgagaactctcccgaactgtacgactctgaa
gatgaagatgaatggaaacaagaggaagagttcactgaaggagcaatgggcaaacgaagc
attgctctcggacgaagcggattccgccctggaaaacggagcatggacaactttcacact
gtggatgttagtgatctcatcatgaaacgatcaatggcaatgggaagattgggactgcgt
ccgggcaagcgttcaatggcctacggcagacaaggattccggccgggaaagagatccatg
gcatatggacggcaaggattccggccaggaaagcgctcaatggcctacggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCTTTGCTCTGCGCAGTTTATT 244 669 34 Antisense
GAAGCAGTTCCGCTTCAGGTCTATC 366 341 61 Antisense
TCGTCCTGACGAATCATCGGCTGTC 394 603 84 Antisense
ATGTGGTTGTCCTTGAGAACTCTCC 134 51 110 Antisense
CTCCCGAACTGTACGACTCTGAAGA 188 235 131 Antisense
AGCATTGCTCTCGGACGAAGCGGAT 672 77 211 Antisense
CGGAGCATGGACAACTTTCACACTG 607 241 250 Antisense
GAAGATTGGGACTGCGTCCGGGCAA 515 337 317 Antisense
GTCCGGGCAAGCGTTCAATGGCCTA 634 473 332 Antisense
GGCAGACAAGGATTCCGGCCGGGAA 710 533 358 Antisense
AGGAAAGCGCTCAATGGCCTACGGT 250 55 420 Antisense
Sequence Source Affymetrix Proprietary Database